This study aims to determine the sources of indoor particulate matter at the Potala Palace Museum in Tibet, China, and evaluate the potential hazards of these pollutants for cultural relics. Long-term monitoring of indoor and outdoor suspended particulate matter concentrations was conducted, and sediment particle samples were collected. The chemical composition of the deposited particles was determined using X-ray fluorescence analysis (XRF). The outdoor suspended particulate concentration was much lower than that indoors; the indoor PM1-10 concentration was much higher than that outdoors and was less affected by outdoor sources. The sources of indoor deposited particles in the high-plateau museum can be classified into four categories: soil dust brought in by tourists from the outdoors, incense ash, pollution from human activities, and ores. Based on data analysis and discussion, proper ventilation can dilute indoor suspended particulate matter, and the installation of air conditioning systems can control temperature and humidity at 20 °C and about 45–60%, respectively, and reduce the fluctuation value, so as to promote particle deposition and better protect the museum’s cultural relics.