“…Five specimens belonging to the new species as well as five specimens of Ecdyonurus rothschildi were used for DNA extraction to get a 658 bp fragment of the mitochondrial cytochrome oxidase I gene (COI) (see Table 1). DNA extraction, PCR amplification, sequencing and alignment construction were performed according to Benhadji et al (2020) or Martynov et al (2022). One sequence of E. rothschildi was retrieved from GenBank, as well as two sequences of E. aurantiacus and two of E. dispar.…”