Contributions in Puerto Rico to Bean, Phaseolus spp., Research

Beaver, James S.; Jensen, Consuelo Estévez de; Miklas, Phillip N.; Porch, Timothy G.

doi:10.46429/jaupr.v104i1.18280

Cited by 3 publications

(3 citation statements)

References 146 publications

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“…Likewise, UPR-Mp-42 reached a genetic gain for resistance to M. phaseolina close to 23% compared with XAN 176 in both environments, while these values increased to 32% and 38% compared to TARS-MST1 in the greenhouse and the field, respectively. Within the white beans, the most important market class in Puerto Rico (Beaver et al, 2020), UPR-Mp-48 (BAT 477/NY6020-4//PRA154), had a genetic gain for resistance to this fungus between 42% and 50% in the greenhouse and 32% in the field than cultivars Bella and Verano. To the best of our knowledge, UPR-Mp-48 is the first white bean germplasm with intermediate resistance to ASB and determinate growth habit (Beaver et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Screening a newly developed common bean germplasm with improved resistance to ashy stem blight in multiple environments

Viteri

Linares‐Ramírez

2022

Front. Plant Sci.

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Ashy stem blight (ASB) caused by the necrotrophic fungus Macrophomina phaseolina (Tassi) Goidanich is an important disease in common bean (Phaseolus vulgaris L.) in the Americas and worldwide. Low to intermediate levels of ASB resistance exist in cultivated and landrace genotypes of the common bean and the tertiary gene pool. However, cultivars with higher levels of resistance are not yet available. Our objectives were to 1) pyramid higher levels of resistance from multiple parent populations within the primary gene pool and 2) compare the response of the newly developed breeding lines (BL) with known sources of resistance. The BL UPR-Mp-22, UPR-Mp-34, UPR-Mp-42, and UPR-Mp-48, known sources of resistance, and susceptible checks were inoculated twice per plant with the PRI21 M. phaseolina isolate in the greenhouse and field trials conducted in Isabela and Lajas, Puerto Rico. None of the genotypes tested were resistant (mean scores 1–3). However, the new black UPR-Mp-42 and white UPR-Mp-48 BL had an intermediate response (mean scores 4–6) compared to white common bean genotypes ‘Bella,’ NY6020-4, and ‘Verano’ and black bean TARS-MST1 that were susceptible (scores ≥7) in all environments. Andean genotypes A 195, PRA154, PRA155, and UPR-Mp-22 were intermediate in the greenhouse. In contrast, UPR-Mp-34 had significantly lower scores than BAT 477 that had a susceptible reaction in the greenhouse in Isabela and in the field in Lajas and SEA 5 that was susceptible in all environments. These new BL possess an enhanced ASB resistance and may be used to improve common bean cultivars or germplasms of different market classes.

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Section: Discussionmentioning

confidence: 99%

Screening a newly developed common bean germplasm with improved resistance to ashy stem blight in multiple environments

Viteri

Linares‐Ramírez

2022

Front. Plant Sci.

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“…BAT 477 was selected in this study because of the importance of identifying resistant QTL to the direct exposure of the pathogen, and to avoid a confounded effect of QTL expressed in field evaluations that could be associated with drought and heat stresses and/or disease avoidance mechanisms. NY6020-4 was selected because white beans are the most important market class in Puerto Rico (Beaver et al, 2020).…”

Section: Plant Materials and Ril Developmentmentioning

confidence: 99%

Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4

Viteri¹,

Linares²,

Miranda³

et al. 2022

Front. Plant Sci.

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Ashy stem blight (ASB), caused by the fungus Macrophomina phaseolina (Tassi) Goidanich is an important disease of the common bean (Phaseolus vulgaris L.). It is important to identify quantitative trait loci (QTL) for ASB resistance and introgress into susceptible cultivars of the common bean. The objective of this research was to identify QTL and single nucleotide polymorphism (SNP) markers associated with ASB resistance in recombinant inbred lines (RIL) derived from a cross between BAT 477 and NY6020-4 common bean. One hundred and twenty-six F6:7 RIL were phenotyped for ASB in the greenhouse. Disease severity was scored on a scale of 1–9. Genotyping was performed using whole genome resequencing with 2x common bean genome size coverage, and over six million SNPs were obtained. After being filtered, 72,017 SNPs distributed on 11 chromosomes were used to conduct the genome-wide association study (GWAS) and QTL mapping. A novel QTL region of ~4.28 Mbp from 35,546,329 bp to 39,826,434 bp on chromosome Pv03 was identified for ASB resistance. The two SNPs, Chr03_39824257 and Chr03_39824268 located at 39,824,257 bp and 39,824,268 bp on Pv03, respectively, were identified as the strongest markers associated with ASB resistance. The gene Phvul.003G175900 (drought sensitive, WD repeat-containing protein 76) located at 39,822,021 – 39,824,655 bp on Pv03 was recognized as one candidate for ASB resistance in the RIL, and the gene contained the two SNP markers. QTL and SNP markers may be used to select plants and lines for ASB resistance through marker-assisted selection (MAS) in common bean breeding.

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“…For common beans, combining host resistance with appropriate cropping system practices that reduce the prevalence of the vector is a critical strategy for controlling BGYMV ( Coyne et al, 2003 ). Genetics and breeding research on BGYMV resistance ( Beebe and Corrales, 1991 ; Miklas et al, 2006 ; Blair and Morales, 2008 ; Singh and Schwartz, 2010 ; Beaver et al, 2020a ) shows that intra- and inter-racial crosses have combined resistances from diverse sources to generate materials with more effective resistance ( Morales and Niessen, 1988 ; Beebe and Corrales, 1991 ; Morales and Singh, 1993 ; Singh et al, 2000 ). To further expand the diversity of resistance, interspecific crosses were used to transfer resistance from P. coccineus ( Osorno et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

NAC Candidate Gene Marker for bgm-1 and Interaction With QTL for Resistance to Bean Golden Yellow Mosaic Virus in Common Bean

et al. 2021

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Genetic resistance is the primary means for control of Bean golden yellow mosaic virus (BGYMV) in common bean (Phaseolus vulgaris L.). Breeding for resistance is difficult because of sporadic and uneven infection across field nurseries. We sought to facilitate breeding for BGYMV resistance by improving marker-assisted selection (MAS) for the recessive bgm-1 gene and identifying and developing MAS for quantitative trait loci (QTL) conditioning resistance. Genetic linkage mapping in two recombinant inbred line populations and genome-wide association study (GWAS) in a large breeding population and two diversity panels revealed a candidate gene for bgm-1 and three QTL BGY4.1, BGY7.1, and BGY8.1 on independent chromosomes. A mutation (5 bp deletion) in a NAC (No Apical Meristem) domain transcriptional regulator superfamily protein gene Phvul.003G027100 on chromosome Pv03 corresponded with the recessive bgm-1 resistance allele. The five bp deletion in exon 2 starting at 20 bp (Pv03: 2,601,582) is expected to cause a stop codon at codon 23 (Pv03: 2,601,625), disrupting further translation of the gene. A Tm-shift assay marker named PvNAC1 was developed to track bgm-1. PvNAC1 corresponded with bgm-1 across ∼1,000 lines which trace bgm-1 back to a single landrace “Garrapato” from Mexico. BGY8.1 has no effect on its own but exhibited a major effect when combined with bgm-1. BGY4.1 and BGY7.1 acted additively, and they enhanced the level of resistance when combined with bgm-1. Tm-shift assay markers were generated for MAS of the QTL, but their effectiveness requires further validation.

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Contributions in Puerto Rico to Bean, Phaseolus spp., Research

Cited by 3 publications

References 146 publications

Screening a newly developed common bean germplasm with improved resistance to ashy stem blight in multiple environments

Screening a newly developed common bean germplasm with improved resistance to ashy stem blight in multiple environments

Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4

NAC Candidate Gene Marker for bgm-1 and Interaction With QTL for Resistance to Bean Golden Yellow Mosaic Virus in Common Bean

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