Contributions of commercial sorbents to the selectivity in immobilized metal affinity chromatography with Cu(II)

Ren, Diya; Penner, Natasha; Slentz, Benjamin E.; Inerowicz, Halina D.; Rybalko, M. A.; Regnier, Fred E.

doi:10.1016/j.chroma.2003.10.041

Cited by 43 publications

(40 citation statements)

References 19 publications

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“…Lou et al [25] prepared a GMA-co-EDMA monolith to which IDA was immobilised via the reactive epoxide moieties exposed at the monolith surface. Later in 2004, Ren et al [26] immobilised IDA on a GMA-co-EDMA monolith in a disk format (CIM Disk) for separation of histidine-containing peptides.…”

Section: Fabrication and Direct Visualisation Of Imac Stationary Phasmentioning

confidence: 99%

Fabrication and characterisation of capillary polymeric monoliths incorporating continuous stationary phase gradients

Currivan

Connolly

Gillespie

et al. 2010

J of Separation Science

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Polymeric monoliths in capillary formats have been fabricated incorporating a gradient of charged functional groups along their length. Scanning capacitively coupled contactless conductivity detection (sC(4)D) was then used to measure the conductive response of the stationary phase and characterise the relative axial distribution of functional groups along the column length. Gradients of 2-acrylamido-2-methyl-1-propanesulphonic acid were prepared using either photografting methods or by filling a capillary column with segmented plugs of monomer mixtures each containing incrementally higher concentrations of the functional monomer. The utility of sC(4)D as a rapid and non-invasive tool for assessing the slope of a variety of gradient configurations is demonstrated. Repeatability of the sC(4)D measurements was <1.7% RSD. Columns with a gradient of covalently bonded iminodiacetic acid were also produced. Changes in the gradient slope were observed after chelation of copper on the stationary phase via a reduction of the conductive response. The effect upon the observed gradient profile of changing the co-monomer composition during column fabrication was studied.

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Section: Fabrication and Direct Visualisation Of Imac Stationary Phasmentioning

confidence: 99%

Fabrication and characterisation of capillary polymeric monoliths incorporating continuous stationary phase gradients

Currivan

Connolly

Gillespie

et al. 2010

J of Separation Science

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“…This was followed by the addition of metal ions to this material to form coordination complexes for use in protein separations [88,89]. Similar supports have been placed in capillaries containing GMA/ EDMA monoliths for the separation of histidine-containing proteins by CEC [90].…”

Section: +mentioning

confidence: 99%

“…another study, four commercial IMAC columns charged with Cu 2+ and containing supports ranging from agarose to GMA/EDMA disks were compared in their isolation of histidine-tagged proteins for proteomics [89]. Cryogels containing IDA and Cu 2+ have been utilized to purify histidine-tagged lactate dehydrogenase [73] and Escherichia coli cells [74].…”

Section: Imacmentioning

confidence: 99%

Affinity monolith chromatography

Mallik

Hage²

2006

J of Separation Science

190

282

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The combined use of monolithic supports with selective affinity ligands as stationary phases has recently given rise to a new method known as affinity monolith chromatography (AMC). This review will discuss the basic principles behind AMC and examine the types of supports and ligands that have been employed in this method. Approaches for placing affinity ligands in monoliths will be considered, including methods based on covalent immobilization, biospecific adsorption, entrapment, and the formation of coordination complexes. Several reported applications will then be presented, such as the use of AMC for bioaffinity chromatography, immunoaffinity chromatography, immobilized metal-ion affinity chromatography, dye-ligand affinity chromatography, and biomimetic chromatography. Other applications that will be discussed are chiral separations and studies of biological interactions based on AMC.

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“…Regarding new approaches one should also mention Convective Interaction Media monolithic discs (CIM discs) from BIASeparations, reported to enable immobilization of IDA [41], although the more elaborated larger scale modules are available for ionic and hydrophobic chromatography [38,42]. Currently the applicability of CIM disc monolythic columns for large scale cannot be yet fully assessed, however, good chemical stability, high resolution and especially low backpressure at high flow rates make this material promising for rapid purification of proteins especially when short residential time is needed.…”

Section: Reviewmentioning

confidence: 99%

Potential for Using Histidine Tags in Purification of Proteins at Large Scale

Gaberc-Porekar

Menart²

2005

Chem Eng & Technol

115

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Attachment of oligo-histidine tag (His-tag) to the protein N-or C-terminus is a good example of early and successful protein engineering to design a unique and generalized purification scheme for virtually any protein. Thus relatively strong and specific binding of His-tagged protein is achieved on an Immobilized Metal-Ion Affinity Chromatography (IMAC) matrix. Most popular hexa-histidine tag and recently also deca-histidine tag are used in combination with three chelating molecules: iminodiacetic acid (IDA), nitrilotriacetic acid (NTA), and carboxymethyl aspartic acid (CM-Asp), covalently attached to the chromatographic matrix. The following combinations with divalent metal ions are preferentially used: (Cu, Zn, Ni, Co)-IDA, Ni-NTA, and Co-CM-Asp. At large scale, regarding cost and product purity, a decisive step is precise and efficient cleavage of His-tag by the cleavage enzyme. Two-step IMAC followed by a polishing step appears to be a minimum but still realistic as an approach to generic technology also for more demanding products. Possible drawbacks in using His-tags and IMAC, such as leaching of metal ions, inefficient cleavage, and batch-to-batch reproducibility must be carefully evaluated before transferred to large scale. Although a great majority of reports refer to small laboratory scale isolations for research purposes it appears there is much higher potential for more extensive use of His-tags and IMAC at large scale than currently documented.

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Contributions of commercial sorbents to the selectivity in immobilized metal affinity chromatography with Cu(II)

Cited by 43 publications

References 19 publications

Fabrication and characterisation of capillary polymeric monoliths incorporating continuous stationary phase gradients

Fabrication and characterisation of capillary polymeric monoliths incorporating continuous stationary phase gradients

Affinity monolith chromatography

Potential for Using Histidine Tags in Purification of Proteins at Large Scale

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