Contributions to automated trace analysis

The aim of this study was to assess the effects of chronic combined exposure to low, environmental doses of Cd, Pb, and Mn on oxidative stress in the liver and heart of rats and on their liver function parameters. Male Wistar rats were divided randomly into eight groups. For nine months controls were receiving drinking water alone, whereas the exposed groups were receiving drinking water with Pb (0.2 mg L -1 ), Cd (1 mg L -1 ), and Mn (2 mg L -1 ) alone or in combinations. Malondialdehyde (MDA) significantly increased in both heart and liver of the animals after combined exposure to metals. Heart MDA correlated with blood Cd, Pb, and Mn and liver MDA with blood Cd. Aspartate aminotransferase (AST) activity and bilirubin concentration also increased significantly in the animal group exposed to all three metals and correlated positively with blood Cd, Pb, and Mn. Our study has confirmed the synergistic effect of the Cd, Mn, and Pb combination on the increase in heart MDA. A similar synergy was observed for Pb+Mn in the increase of serum alanine aminotransferase (ALT) activity as an indicator of liver function.

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“…The method has been developed by Stoepplers et al (22,23). Blood was deproteinised with 0.8 mol L -1 of nitric acid.…”

Section: Metals In Whole Bloodmentioning

confidence: 99%

Effects of chronic exposure to lead, cadmium, and manganese mixtures on oxidative stress in rat liver and heart / Utjecaj kronične istodobne izloženosti olovu, kadmiju i manganu na oksidativni stres u jetri i srcu štakora

Markiewicz−Górka

Januszewska

Michalak

et al. 2015

Archives of Industrial Hygiene and Toxicology

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“…Following collection, blood samples from Redberry Lake females were transferred to nitric acid-washed vials, kept cool in the field, and frozen pending further processing. Mercury (Hg), Selenium (Se), and Lead (Pb) analyses were conducted at Atlantic Environment Canada's National Wildlife Research Centre according to methods described by Neugebauer et al (2003); Cadmium (Cd) was analyzed at the Institut National de Santé Publique du Québec according to Stoeppler and Brandt (1980). Cd concentrations were expressed on a volumetric basis, whereas Hg, Se, and Pb concentrations were expressed on a wet weight basis.…”

Section: Laboratory Methodsmentioning

confidence: 99%

Full Issue

2014

The Condor

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“…The IUPAC reference method for nickel in urine calls for acidification of urine quality control samples with nitric acid and storage in polypropylene tubes with a screw-cap at -200C (22). No adsorption of cadmium onto container walls (type not specified) was seen from urine acidified to pH<2 (151).…”

Section: Variation Associated With Specimen Collection and Storagementioning

confidence: 99%

“…Hydrochloric and nitric acids have been used as preservatives for urine specimens for mercury and cadmium analysis (eg 57,151), and for analysis of metals in general in water samples. mhey -especially H1\103 -have an important role in the present day electrothermal atomic absorption analysis methods, too.…”

Section: Contaminationmentioning

confidence: 99%