Contributions to membrane-embedded-protein diffusion beyond hydrodynamic theories

Camley, Brian A.; Brown, Frank L. H.

doi:10.1103/physreve.85.061921

Cited by 36 publications

(47 citation statements)

References 67 publications

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“…Finally, if a protein distorts the surrounding membrane, additional drag may be caused, reducing the protein's diffusion coefficient. [66][67][68][69][70] If these effects are strong enough, they could potentially compete with or even overwhelm the hydrodynamic contributions to D discussed in this work.…”

Section: The Saffman-delbrück Model Is a Hydrodynamic Modelmentioning

confidence: 99%

Strong influence of periodic boundary conditions on lateral diffusion in lipid bilayer membranes

Camley

Lerner

Pastor

et al. 2015

The Journal of Chemical Physics

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125

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The Saffman-Delbrück hydrodynamic model for lipid-bilayer membranes is modified to account for the periodic boundary conditions commonly imposed in molecular simulations. Predicted lateral diffusion coefficients for membrane-embedded solid bodies are sensitive to box shape and converge slowly to the limit of infinite box size, raising serious doubts for the prospects of using detailed simulations to accurately predict membrane-protein diffusivities and related transport properties. Estimates for the relative error associated with periodic boundary artifacts are 50% and higher for fully atomistic models in currently feasible simulation boxes. MARTINI simulations of LacY membrane protein diffusion and LacY dimer diffusion in DPPC membranes and lipid diffusion in pure DPPC bilayers support the underlying hydrodynamic model. C 2015 AIP Publishing LLC. [http://dx

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Section: The Saffman-delbrück Model Is a Hydrodynamic Modelmentioning

confidence: 99%

Strong influence of periodic boundary conditions on lateral diffusion in lipid bilayer membranes

Camley

Lerner

Pastor

et al. 2015

The Journal of Chemical Physics

Self Cite

125

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“…7 and references therein). Such studies have systematically gone beyond the SD model by including additional effects (8)(9)(10)(11)(12). So far, a thorough verification of these ideas has not been attempted.…”

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confidence: 99%

Shape matters in protein mobility within membranes

Quemeneur

Sigurdsson

Renner

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

106

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The lateral mobility of proteins within cell membranes is usually thought to be dependent on their size and modulated by local heterogeneities of the membrane. Experiments using single-particle tracking on reconstituted membranes demonstrate that protein diffusion is significantly influenced by the interplay of membrane curvature, membrane tension, and protein shape. We find that the curvature-coupled voltage-gated potassium channel (KvAP) undergoes a significant increase in protein mobility under tension, whereas the mobility of the curvature-neutral water channel aquaporin 0 (AQP0) is insensitive to it. Such observations are well explained in terms of an effective friction coefficient of the protein induced by the local membrane deformation.Brownian motion | Saffman-Delbrück | internal membrane structure | drag force | micropipette aspiration B rownian motion plays an essential role in biological processes. Since the pioneering experiments of Perrin (1), the observation of diffusing objects has emerged as a mean to extract the rheological properties of the surrounding medium or the probe particle size. The theoretical investigation of diffusion of proteins within membranes has been studied widely going back to P. G. Saffman and M. Delbrück (SD). They investigated the hydrodynamic drag acting on a membrane inclusion when the membrane is described as a 2D fluid sheet of viscosity μ m embedded within a less viscous fluid of viscosity η (2). In this theory, the diffusion coefficient D 0 in the limit of a large viscosity contrast between the membrane and bulk fluid is given by:The length ℓ = μ m =η is the length scale over which flow is generated within the bilayer by the inclusion, k B T is the thermal energy, and γ is Euler's constant. This model predicts a logarithmic dependence of D 0 on the protein radius a p , which has been confirmed for some in vitro experiments on membranes containing transmembrane proteins (see ref.3 and references therein). In contrast, the experiments of Gambin et al. (4) showed significant deviations from the SD theory. A possible origin for the discrepancy observed by Gambin et al. (4) is the significant local membrane deformation due to the interaction between the inclusion and the lipid bilayer (5). Naji et al. suggested in ref. 6 that inclusions experience additional dissipation, either due to internal flows within the membrane or to additional fluid flows produced by the deformed membrane. This work triggered a number of theoretical studies investigating the coupling of inclusion proteins with the membrane that had been pioneered by the Seifert's group (see ref. 7 and references therein). Such studies have systematically gone beyond the SD model by including additional effects (8-12). So far, a thorough verification of these ideas has not been attempted. To investigate the effect of the protein-lipid coupling on the protein mobility, we study its dependence on membrane tension, because this parameter affects the local membrane deformation.In this work, we compare the mobility of t...

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“…The density difference between the two components of the mixture explicitly generates the conserved order parameter, unlike in previous studies. 23,24) We formulate our problem in Sect. 2, and solve the problem in Sect.…”

Section: Introductionmentioning

confidence: 99%

Drag Coefficient of a Raftlike Domain Embedded in a Fluid Membrane Being a Near-Critical Binary Mixture

Fujitani

2013

J. Phys. Soc. Jpn.

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We consider a circular liquid domain in a flat fluid membrane surrounded by three-dimensional fluids, and assume that the membrane outside the domain is a two-dimensional near-critical binary fluid mixture. The membrane viscosity outside the domain is assumed to be uniform, irrespective of the local composition, and to be the same as that of the domain. Using the Gaussian free-energy functional, we show that the drag coefficient of the domain can be decreased by the preferential attraction between the domain and a component of the mixture, unlike that of a rigid sphere in a three-dimensional near-critical binary fluid mixture studied recently.

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Contributions to membrane-embedded-protein diffusion beyond hydrodynamic theories

Cited by 36 publications

References 67 publications

Strong influence of periodic boundary conditions on lateral diffusion in lipid bilayer membranes

Strong influence of periodic boundary conditions on lateral diffusion in lipid bilayer membranes

Shape matters in protein mobility within membranes

Drag Coefficient of a Raftlike Domain Embedded in a Fluid Membrane Being a Near-Critical Binary Mixture

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