Control Efficacy of Jingdusha against Corynespora cassiicola (Hyphomycetales: Dematiaceae) on Cucumber

Abstract

Aims: The paper aimed to clarify the effect of cucumber target leaf spot (TLS) under the Jingdusha (JDS) treatment.
 Study Design: We applied the method of artificial inoculation in the pot, and analyzed the changes in growth indexes and physiological characteristics.
 Place and Duration of Study: In 2018, these experiments were conducted in College of Bioscience and Biotechnology of Shenyang Agricultural University (Lab 240).
 Methodology: The seedlings in the two-leaf period were induced by the best application scheme of JDS, then inoculated Corynespora cassiicola for 24 h. Cucumber seedlings of each treatment group were randomly selected for photographing and growth index determination after inoculation for 5 d. The leaves of cucumber seedlings in each treatment group were randomly collected at 1 d, 3 d, 5 d, 7 d, and 9 d after inoculation for the determination of physiological and biochemical indicators.
 Results: When C. cassiicola infects cucumber, JDS can effectively improve the growth and photosynthetic pigment content of cucumber, reduce the degradation of chlorophyll (Chl) under the stress of C. cassiicola, strengthen the variety of metabolic responses in the plant, repair the enzyme protection system of cucumber leaves, reduce the accumulation of reactive oxygen species, shorten the process of membrane lipid peroxidation in blades.
 Conclusion: Taken together, these results suggest that JDS can improve the resistance of cucumber seedlings to C. cassiicola by regulating growth indexes and physiological characteristics. This work will provide a theoretical basis for further elucidating the molecular mechanism of JDS in cucumber defense against C. cassiicola.

The Effect of Jingdusha on Cucumber Chlorophyll Fluorescence Parameters under Corynespora cassiicola Stress

“…A previous study [6] found that JDS did not kill C. cassiicola by itself but had a significant resistance-inducing effect on cucumber seedlings. JDS at 2.0 mL•L -1 induced cucumber three times every 7 d interval for 1 d showed the highest efficacy of 68.93% against C. cassiicola [7]. To clarify the mechanism of resistance induction of cucumber TLS, we analyzed the changes of physiological and biochemical substances in cucumber plants after the optimal application program and then elucidated the mechanism of resistance induction of JDS to C. cassiicola.…”