Control Measurements of Escherichia coli Biofilm: A Review

Zhou, Feng; Wang, Dehua; Hu, Jiamiao; Zhang, Yi; Tan, Bee K.; Lin, Shaoling

doi:10.3390/foods11162469

Cited by 25 publications

(20 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bio lms are also associated to recurrent UTIs [27]. Bio lms are then a major public health problem that should be studied, monitored and eradicated especially in hospital settings [28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

High prevalence of virulence genes and in-vitro biofilm production in clinical multidrug resistant Escherichia coli in Dakar Senegal

Dossouvi

Lô

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Bacterial virulence is a key factor determining the outcome of each bacterial infection and virulent bacteria are often associated to high-risk infections. Extraintestinal pathogenic Escherichia coli (ExPEC) is the most implicated bacterium in human bacterial infections and its virulence factors are classified into five categories: adhesins, toxins, iron capture systems, protectins and invasins. Furthermore, bacterial biofilms are the main cause of hospital-acquired infections like urinary catheter-associated infections, valve endocarditis, Otitis and cystic fibrosis.Results For the sixteen virulence genes sought by standard polymerase chain reaction (PCR), all the 78 ExPECs isolates carried at least four virulence genes. Following prevalences of virulence genes were reported: adhesins genes fimH (98.7%), mrkD (98.7%), papC (46.2%), afaC (9%), sfa / focDE (1.3%); iron acquisition systems genes entB (98.7%), fepA (98.7%), ybtS (93.6%), fyuA (91%), iucA (91%), iucB (91%), iutA (34.6), iroB (6.4%), iroN (6.4%) and toxins genes hlyA (10.3%), cnf (1 & 2) (10.3%). Seventy-five out of 78 isolates (96.2%) carried at least the combination of two adhesins genes and two iron capture systems genes whereas 8 out of 78 (10.3%) harbored the combination of (adhesins genes + iron acquisition systems genes + toxins genes). Among the 78 strains studied, one hospital-acquired strain isolated from urine harbored 15 virulence genes out of 16 sought. The evaluation of biofilm-formation capacity revealed that all (29/29) hospital-acquired isolates were biofilm producers with (6/29; 20.7%) strong biofilm producers, (15/29; 51.7%) moderate biofilm producers and (8/29; 27.6%) weak biofilm producers. Isolates carrying papC had greater biofilm formation capacity than those not carrying papC (p < 0.001).Conclusions Most of our strains had moderate biofilm-formation capacity and carried an average of 9 virulence genes out of 16 sought. These eight strains carrying a combination of genes (adhesins + iron acquisition systems genes + toxins genes) may be hypervirulent isolates. Additional studies may confirm this. The deepening of this kind of study on bacterial virulence and hospital bacterial biofilms could lead to the improvement of infections investigation, prevention and therapeutic protocols.

show abstract

Section: Introductionmentioning

confidence: 99%

High prevalence of virulence genes and in-vitro biofilm production in clinical multidrug resistant Escherichia coli in Dakar Senegal

Dossouvi

Lô

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…E. coli is often associated with biofilm formation [ 23 , 24 , 25 , 26 ], although our research shows that the ability of bacteria to form a biofilm is highly variable depending on each individual strain. In the human body, bacteria can attach to tissues and to artificial surfaces, such as catheters and implants, to form a biofilm [ 11 ].…”

Section: Discussionmentioning

confidence: 96%

Anti-Biofilm Effect of Bacteriophages and Antibiotics against Uropathogenic Escherichia coli

et al. 2022

View full text Add to dashboard Cite

Escherichia coli is a common cause of biofilm-associated urinary tract infections. Bacteria inside the biofilm are more resistant to antibiotics. Six E. coli strains isolated from patients with urinary tract infections were screened for biofilm-forming capability and antimicrobial susceptibility. Two of the most significant biofilm-producing strains were selected for minimal inhibitory concentration and minimal biofilm eradication concentration in vitro testing using amoxicillin–clavulanic acid, ciprofloxacin, and three commercial bacteriophage cocktails (Pyobacteriophag, Ses, and Intesti). In case of a low phage effect, an adaptation procedure was performed. Although the biofilms formed by strain 021UR were resistant to amoxicillin–clavulanic acid and ciprofloxacin, the three phage cocktails were able to reduce biofilm formation. In contrast, phages did not affect the 01206UR strain against planktonic and biofilm-forming cells. After Pyobacteriophag adaptation, the effect improved, and, regardless of the concentration, the adapted phage cocktail could destroy both planktonic cells and the biofilm of strain 01206UR. Bacteriophages capable of killing bacteria in biofilms can be used as an alternative to antibiotics. However, each case should be considered individually due to the lack of clinical trials for phage therapy. Antimicrobial and phage susceptibility should be determined in biofilm models before treatment to achieve the desired anti-biofilm effect.

show abstract

“…Biofilm formation by AMR E. coli cells can pose a serious threat in human health and food industry because this structure is generally resistant to the human immune system and shows increased-tolerance against antibiotic treatments (Sharma et al, 2016;Zhou et al, 2022). Bacteriophages are known to efficiently remove biofilms (Pires et al, 2017), and the ability of EJP2 to eradicate biofilms formed by AMR E. coli was tested.…”

Section: Biofilm Inhibition Assaymentioning

confidence: 99%

Characterization and genomic study of EJP2, a novel jumbo phage targeting antimicrobial resistant Escherichia coli

Kim

Lee

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

The emergence of antimicrobial resistance (AMR) Escherichia coli has noticeably increased in recent years worldwide and causes serious public health concerns. As alternatives to antibiotics, bacteriophages are regarded as promising antimicrobial agents. In this study, we isolated and characterized a novel jumbo phage EJP2 that specifically targets AMR E. coli strains. EJP2 belonged to the Myoviridae family with an icosahedral head (120.9 ± 2.9 nm) and a non-contractile tail (111.1 ± 0.6 nm), and contained 349,185 bp double-stranded DNA genome with 540 putative ORFs, suggesting that EJP2 could be classified as jumbo phage. The functions of genes identified in EJP2 genome were mainly related to nucleotide metabolism, DNA replication, and recombination. Comparative genomic analysis revealed that EJP2 was categorized in the group of Rak2-related virus and presented low sequence similarity at the nucleotide and amino acid level compared to other E. coli jumbo phages. EJP2 had a broad host spectrum against AMR E. coli as well as pathogenic E. coli and recognized LPS as a receptor for infection. Moreover, EJP2 treatment could remove over 80% of AMR E. coli biofilms on 96-well polystyrene, and exhibit synergistic antimicrobial activity with cefotaxime against AMR E. coli. These results suggest that jumbo phage EJP2 could be used as a potential biocontrol agent to combat the AMR issue in food processing and clinical environments.

show abstract

Control Measurements of Escherichia coli Biofilm: A Review

Cited by 25 publications

References 63 publications

High prevalence of virulence genes and in-vitro biofilm production in clinical multidrug resistant Escherichia coli in Dakar Senegal

High prevalence of virulence genes and in-vitro biofilm production in clinical multidrug resistant Escherichia coli in Dakar Senegal

Anti-Biofilm Effect of Bacteriophages and Antibiotics against Uropathogenic Escherichia coli

Characterization and genomic study of EJP2, a novel jumbo phage targeting antimicrobial resistant Escherichia coli

Contact Info

Product

Resources

About