2024
DOI: 10.1101/2024.05.04.592262
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Control of 3ʹ splice site selection by the yeast splicing factor Fyv6

Katherine A. Senn,
Karli A. Lipinski,
Natalie J. Zeps
et al.

Abstract: Pre-mRNA splicing is catalyzed in two steps: 5′ splice site (SS) cleavage and exon ligation. A number of proteins transiently associate with spliceosomes to specifically impact these steps (1st and 2nd step factors). We recently identified Fyv6 (FAM192A in humans) as a 2nd step factor in S. cerevisiae; however, we did not determine how widespread Fyv6's impact is on the transcriptome. To answer this question, we have used RNA-Seq to analyze changes in splicing. These results show that loss of Fyv6 results in a… Show more

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