Control of Accelerated Vein Graft Atheroma with the Nitric Oxide Precursor: l-Arginine

Herbal Viagra and nitric oxide: how hard is the evidence?

“…Because L-arginine exists in the plasma at concentrations of about 100uM, the concentration of L-arginine should not be rate-limiting. But despite the calculations of enzymologists, and in apparent contradiction of MichaelisMenton kinetics, our observations were confirmed by a number of other groups [40][41][42][43][44][45]. In a rather broad range of cardiovascular disorders, it appears that the endothelial vasodilator dysfunction observed in these conditions can be reversed, at least in the short-term, by oral or intravenous administration of L-arginine supplementation.…”

Reduction of Lipid Peroxidation with Intraoperative Superoxide Dismutase Treatment Decreases Intimal Hyperplasia in Experimental Vein Grafts

“…Previous studies from our laboratory have shown that 28-day vein grafts (untreated) are insensitive to acetylcholine, reflecting a lack of endothelial-dependent nitric oxide biosynthesis [27][28][29]. We had postulated that this may be due, in part, to increased free radical generation.…”

Local Inhibition of Tyrosine Kinase Activity Markedly Attenuates the Development of Intimal Hyperplasia in Experimental Vein Grafts

“…The animals were sacrificed with an overdose of barbiturates. (122 mM NaCl, 4.7 mM KCl, 1.2 mM MgCl 2 , 2.5 mM CaCl 2 , 15.4 mM NaHCO 3 , 1.2 mM KH 2 PO 4 , and 5.5 mM glucose, maintained at Animal care complied with the ''Principles of Laboratory Animal 37ЊC and oxygenated with 95% O 2 and 5% CO 2 ) as previously deCare'' as formulated by the National Society for Medical Research scribed [13]. Following equilibration, the resting tension was adand the ''Guide for the Care and Use of Laboratory Animals'' issued justed in increments from 0. cle cells from the media of the thoracic aorta of male New Zealand followed by sonication in ice-cold lysis buffer (1:4, w:v; 50 mM TrisWhite rabbits were isolated and grown in tissue culture.…”