Control of adventitious root formation: insights into synergistic and antagonistic hormonal interactions

Lakehal, Abdellah; Bellini, Catherine

doi:10.1111/ppl.12823

Cited by 168 publications

(148 citation statements)

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“…Both pathways act synergistically in control of the JA response, as 335 indicated by the much lower numbers of ARs produced by ninjamyc2-322B double mutants 336 than the parental lines (ninja and myc2-322B) and wild-type controls. Therefore, the strong 337 phenotype of ninjamyc2-322B double mutants may be due to de-repression of not only MYC2, 338 and model systems (Lakehal and Bellini, 2018). In this study, we genetically demonstrated that 358 depleting CKs by either blocking their biosynthesis or enhancing their degradation restores the 359 ARI wild-type phenotype in an ERF115-overexpressing line, confirming that ERF115 represses 360 ARI through CK signaling.…”

supporting

confidence: 56%

ETHYLENE RESPONSE FACTOR 115integrates jasmonate and cytokinin signaling machineries to repress adventitious rooting inArabidopsis

Lakehal

Dob

Rahneshan

et al. 2019

Preprint

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34Jasmonate (JA), an oxylipin-derived phytohormone, plays crucial roles not only in plant 35 immunity and defense against herbivorous insects but also in plant growth and developmental 36 processes, including regeneration and organogenesis. However, the mechanistic basis of its 37 mode of action and precise role in integrating other signaling cues are poorly understood. Here 38 we provide genetic indications that JA signaling acts in both NINJA-dependent and -39 independent modulation of the transcriptional activity of MYC transcription factors involved 40 in the inhibition of adventitious root initiation (ARI). Our data show that NINJA-dependent JA 41 signaling in pericycle cells blocks early events of ARI. Moreover, transcriptomic comparison 42 of ninja-1myc2-322B double mutant (which produce extremely few ARs) and wild type 43 seedlings identified a novel molecular network governed by the APETALA2/ETHYLENE 44 RESPONSE FACTOR 115 (ERF115) transcription factor. We demonstrate that JA-induced 45 ERF115 activates the cytokinin signaling machinery and thereby represses ARI. Altogether, 46 our results reveal a molecular network involving cooperative crosstalk between JA and CK 47 machineries that inhibits ARI. 48 49 Key words: 50 Jasmonate, cytokinins, adventitious rooting, AP2/ERF transcription factors, de novo 51 organogenesis. 52 53 Jasmonate (JA), a stress-induced phytohormone, plays crucial roles in plant immunity and 54 defense against herbivorous insects (Wasternack and Hause, 2013). It also participates in 55 control of diverse developmental processes, including tissue regeneration and rhizotaxis 56 (Wasternack and Hause, 2013;Lakehal et al., 2020). The isomer (+)-7-iso-JA-Ile (JA-Ile), the 57 bioactive form of JA (Fonseca et al., 2009), is perceived by the F-box protein CORONATINE 58 INSENSITIVE1 (COI1), which is an integral component of the Skp-Cullin-F-box (SCF) 59 complex (Xie et al., 1998). The COI1 receptor fine-tunes the function of the JA transcriptional 60 machinery in a simple manner. Briefly, in the resting state, marked by low JA-Ile contents, the 61 transcriptional activity of a number of transcription factors, including the basic-Helix-loop-62 Helix MYC, is repressed by JASMONATE ZIM DOMAIN (JAZ) repressors through either 63 physical interaction or recruitment of the general co-repressor TOPLESS (TPL) or TPL-related 64 proteins (TPRs) (Chini et al., 2007; Thines et al., 2007; Yan et al., 2007). The adaptor NOVEL 65 INTERACTOR OF JAZ (NINJA) mediates interaction of JAZs with TPL or TRPs (Pauwels et 66 al., 2010). During activation, marked by accumulation of JA-Ile, JAZs form co-receptor 67 complexes with COI1. This interaction is facilitated by JA-Ile, which acts as a molecular glue 68 (Sheard et al., 2010). Formation of the co-receptor complexes triggers ubiquitylation and 69 proteasome-dependent degradation of the targeted JAZs, thereby releasing the transcription 70 factors to transcriptionally induce or repress their downstream target genes. Biochemical 71 studies suggest that JAZ-dependent ...

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supporting

confidence: 56%

ETHYLENE RESPONSE FACTOR 115integrates jasmonate and cytokinin signaling machineries to repress adventitious rooting inArabidopsis

Lakehal

Dob

Rahneshan

et al. 2019

Preprint

Self Cite

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“…It is well known that auxin can stimulate adventitious rooting in several plant species 308 (Lakehal & Bellini, 2019), therefore we tested whether different types of auxin could 309 stimulate ARI under cWL. Three-week-old de-rooted seedlings were treated with 1 or 310 5 µM of Indole 3-Acetic Acid (IAA), 1-Naphthalene 3-Acetic Acid (1-NAA) or Indole 1B-D).…”

Section: Hormone Profiling Experiments 249mentioning

confidence: 99%

Red light controls adventitious root regeneration by modulating hormone homeostasis inPicea abiesseedlings

Alallaq

Ranjan

Brunoni

et al. 2020

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Vegetative propagation relies on the capacity of plants to regenerate de novo adventitious roots (ARs), a quantitative trait controlled by the interaction of endogenous factors such as hormones and environmental cues among which light plays a central role. However, the physiological and molecular components mediating light cues during AR initiation (ARI) remain largely elusive. We explored the effect of light spectral quality on ARI in de-rooted Norway spruce seedlings as well as on hormone metabolism with sensitive mass spectrometry-based methods. We coupled this to gene expression analysis to identify potential signaling pathways and to extensive anatomical characterization to investigate ARI at the cellular level. We showed that in contrast to white light and blue light, red light promoted ARI likely by reducing jasmonate (JA) and JA-isoleucine biosynthesis and repressing the accumulation of isopentyl-adenine-type cytokinins and abscisic acid. We confirmed that exogenously applied JA and/or CK inhibit ARI, and found that they possibly act in the same pathway. The negative effect of JA was confirmed at the histological level. We showed that JA represses the early events of ARI. In conclusion, RL promotes ARI by repressing the accumulation of the wound-induced phytohormones JA and CK.HighlightBlue and red light have an opposite effect on adventitious root initiation in Norway spruce hypocotyl, red light having a promoting effect by modulating hormone homeostasis.

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“…Although this requires effective rooting of stem cuttings, this is often not achieved and many studies conducted at physiological, biochemical and molecular levels to better understand the entire process have shown that AR formation is a heritable quantitative genetic trait controlled by multiple endogenous and environmental factors. In particular, it has been shown to be controlled by complex hormone cross-talks, in which auxin plays a central role (36,37). The specificity of auxin response is thought to depend on a specific combinatorial suite of ARF-Aux/IAA protein-protein interactions from among the huge number of potential interactions that modulate the auxin response of gene promoters via different affinities and activities (reviewed in (3,6)).…”

Section: Discussionmentioning

confidence: 99%

Molecular framework for TIR1/AFB-Aux/IAA-dependent auxin sensing controlling adventitious rooting in Arabidopsis

Lakehal

Chaabouni

Cavel

et al. 2019

Preprint

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In Arabidopsis thaliana, canonical auxin-dependent gene regulation is mediated by 23 transcription factors from the AUXIN RESPONSE FACTOR (ARF) family, most of which interact with 29 auxin/indole acetic acid (Aux/IAA) repressors, themselves forming, in the presence of auxin, coreceptor complexes with one of six TRANSPORT INHIBITOR1/AUXIN-SIGNALLING F-BOX PROTEINS (TIR1/AFB). Different combinations of co-receptors drive specific sensing outputs, allowing auxin to control a myriad of processes. Considerable efforts have been made to discern the temporal and spatial specificity of auxin action. However, owing to a lack of obvious phenotype in single loss-of-function mutants in Aux/IAA genes, most genetic studies have relied on gain-of-function mutants, which are highly pleiotropic. In this article, we describe a molecular framework for the role of several members of the auxin sensing machinery.Using loss-of-function mutants, we demonstrate that TIR1 and AFB2 are positive regulators, whereas IAA6, IAA9 and IAA17 are negative regulators of adventitious root (AR) formation. The three Aux/IAA proteins interact with ARF6 and/or ARF8, which we have previously shown to be positive regulators of AR formation upstream of jasmonate, and likely repress their activity. Our data also suggest a dual role for TIR1 in the control of JA biosynthesis and conjugation, as revealed by upregulation of several JA biosynthesis genes in the tir1-1 mutant. In conclusion, we propose that in the presence of auxin, TIR1 and AFB2 form specific sensing complexes with IAA6, IAA9 and/or IAA17 that modulate JA homeostasis to control AR initiation.

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Control of adventitious root formation: insights into synergistic and antagonistic hormonal interactions

Cited by 168 publications

References 74 publications

ETHYLENE RESPONSE FACTOR 115integrates jasmonate and cytokinin signaling machineries to repress adventitious rooting inArabidopsis

ETHYLENE RESPONSE FACTOR 115integrates jasmonate and cytokinin signaling machineries to repress adventitious rooting inArabidopsis

Red light controls adventitious root regeneration by modulating hormone homeostasis inPicea abiesseedlings

Molecular framework for TIR1/AFB-Aux/IAA-dependent auxin sensing controlling adventitious rooting in Arabidopsis

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