Control of angiogenesis by VEGF and endostatin-encapsulated protein microcrystals and inhibition of tumor angiogenesis

Matsumoto, Goichi; Hirohata, Rie; Hayashi, Kousuke; Sugimoto, Yuki; Kotani, Eiji; Shimabukuro, Junji; Hirano, Toshio; Nakajima, Yoshikazu; Kawamata, Shin; Mori, Hajime

doi:10.1016/j.biomaterials.2013.10.051

Cited by 24 publications

(26 citation statements)

References 35 publications

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“…We previously showed that the activity of cytokines encapsulated in polyhedra, which are protein microcrystals of cypovirus, can be stably maintained over long periods in both ex vivo and in vivo environments, and can be slowly released to stimulate the growth and differentiation of many cell types continually (14,(24)(25)(26)(27)(28)(29). Therefore, to determine whether sericin hydrogels can act as scaffolds to support cell growth and differentiation, we cultivated the mouse embryonic stem (ES) cell line EB5 on sericin hydrogels that were overlaid with a medium containing canonical recombinant human leukemia inhibitory factor (rhLIF) or that incorporated polyhedron-encapsulated LIF (24) (SI Appendix, SI Materials and Methods).…”

Section: Expression and Physiological Effects Of Truncated P1a In Silmentioning

confidence: 99%

Bioengineered silkworms with butterfly cytotoxin-modified silk glands produce sericin cocoons with a utility for a new biomaterial

Otsuki

Yamamoto

Matsumoto

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Significance Specific gene functions have been successfully suppressed by gene silencing or editing in many organisms. However, genetic manipulation to suppress the function of a target tissue has not been achieved using cytotoxin genes. We established transgenic silkworms with posterior silk glands (PSGs) that express the enzymatic domain of the cytotoxin pierisin-1A (P1A). The larvae with the modified PSGs produced the sericin cocoons with potential utilities in tissue engineering. The targeted P1A expression was found to cause site-specific repression of certain protein synthesis that appeared to have no impact on the developmental stages of individuals. Thus, the new approach through targeted P1A expression could be applicable to the development of biologically useful model organisms with tissue-specific dysfunction.

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Section: Expression and Physiological Effects Of Truncated P1a In Silmentioning

confidence: 99%

Bioengineered silkworms with butterfly cytotoxin-modified silk glands produce sericin cocoons with a utility for a new biomaterial

Otsuki

Yamamoto

Matsumoto

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…This technique was applied to immobilize various cytokines, such as FGF, LIF, and EGF in PhCs. 126,127 These immobilized cytokines retain their biological activities, which include promotion of fibroblast growth and proliferation of mouse embryonic and induced pluripotent stem cells by slow release of the immobilized proteins.…”

Section: In Vivo Crystal Engineeringmentioning

confidence: 99%

Design of a confined environment using protein cages and crystals for the development of biohybrid materials

2016

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There is growing interest in the design of protein assemblies for use in materials science and bionanotechnology. Protein assemblies, such as cages and crystalline protein structures, provide confined chemical environments that allow immobilization of metal complexes, nanomaterials, and proteins by metal coordination, assembly/disassembly reactions, genetic manipulation and crystallization methods. Protein assembly composites can be used to prepare hybrid materials with catalytic, magnetic and optical properties for cellular applications due to their high stability, solubility and biocompatibility. In this feature article, we focus on the recent development of ferritin as the most promising molecular template protein cage and in vivo and in vitro engineering of protein crystals as solid protein materials with functional properties.

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“…3B). Tube formation is a key step for angiogenesis in vitro 26, 27 . To investigate the angiogenic effect of 5%CS/β-TCP scaffolds on HUVECs, cells were seeded on Matrigel in the lower chamber with 5%CS/β-TCP or β-TCP in transwell inserts.…”

Section: Resultsmentioning

confidence: 99%

3D printed scaffolds of calcium silicate-doped β-TCP synergize with co-cultured endothelial and stromal cells to promote vascularization and bone formation

Deng

Jiang

et al. 2017

Sci Rep

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Synthetic bone scaffolds have potential application in repairing large bone defects, however, inefficient vascularization after implantation remains the major issue of graft failure. Herein, porous β-tricalcium phosphate (β-TCP) scaffolds with calcium silicate (CS) were 3D printed, and pre-seeded with co-cultured human umbilical cord vein endothelial cells (HUVECs) and human bone marrow stromal cells (hBMSCs) to construct tissue engineering scaffolds with accelerated vascularization and better bone formation. Results showed that in vitro β-TCP scaffolds doped with 5% CS (5%CS/β-TCP) were biocompatible, and stimulated angiogenesis and osteogenesis. The results also showed that 5%CS/β-TCP scaffolds not only stimulated co-cultured cells angiogenesis on Matrigel, but also stimulated co-cultured cells to form microcapillary-like structures on scaffolds, and promoted migration of BMSCs by stimulating co-cultured cells to secrete PDGF-BB and CXCL12 into the surrounding environment. Moreover, 5%CS/β-TCP scaffolds enhanced vascularization and osteoinduction in comparison with β-TCP, and synergized with co-cultured cells to further increase early vessel formation, which was accompanied by earlier and better ectopic bone formation when implanted subcutaneously in nude mice. Thus, our findings suggest that porous 5%CS/β-TCP scaffolds seeded with co-cultured cells provide new strategy for accelerating tissue engineering scaffolds vascularization and osteogenesis, and show potential as treatment for large bone defects.

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Control of angiogenesis by VEGF and endostatin-encapsulated protein microcrystals and inhibition of tumor angiogenesis

Cited by 24 publications

References 35 publications

Bioengineered silkworms with butterfly cytotoxin-modified silk glands produce sericin cocoons with a utility for a new biomaterial

Bioengineered silkworms with butterfly cytotoxin-modified silk glands produce sericin cocoons with a utility for a new biomaterial

Design of a confined environment using protein cages and crystals for the development of biohybrid materials

3D printed scaffolds of calcium silicate-doped β-TCP synergize with co-cultured endothelial and stromal cells to promote vascularization and bone formation

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