Control of ATP concentration in Escherichia coli using an ATP-sensing riboswitch for enhanced S-adenosylmethionine production

Chen, Yawei; Zhou, Huiyun; Wang, Meng; Tan, Tianwei

doi:10.1039/c7ra02538f

Cited by 18 publications

(24 citation statements)

References 32 publications

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“…Methylation proceess was further upregulated by regulating ATP and NADPH contents, which are important cofactors for SAM biosynthesis. Previous studies proved that ydaO from B. subtilis can dynamically regulate ATP content in E. coli [ 47 , 48 ]. The accumulation of (2 S )-sakuranetin increased by 162.38% in the ydaO -expressing strain compared with strain 7-FOMT-3.…”

Section: Discussionmentioning

confidence: 99%

Production of (2S)-sakuranetin from (2S)-naringenin in Escherichia coli by strengthening methylation process and cell resistance

Sun

Gao

et al. 2022

Synthetic and Systems Biotechnology

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Section: Discussionmentioning

confidence: 99%

Production of (2S)-sakuranetin from (2S)-naringenin in Escherichia coli by strengthening methylation process and cell resistance

Sun

Gao

et al. 2022

Synthetic and Systems Biotechnology

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“…A newly discovered ATP-sensing riboswitch ydaO motif was used to balance the requirement of ATP in SAM biosynthesis, with the result of a higher SAM titer (1.23 mg/L). The SAM titer was increased by approximately 55% when compared with the control [28].…”

Section: Atp Analysis Of Recombinant K Phaffii Strainsmentioning

confidence: 87%

Knockout of the DAS gene increases S-adenosylmethionine production in Komagataella phaffii

Liu

Zhou

et al. 2020

Biotechnology & Biotechnological Equipment

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S-adenosylmethionine (SAM) is an important compound in living organisms and has a number of different roles, such as polyamine synthesis. It can also be used for the treatment of diseases. Gene targeting with homologous recombination in Komagataella phaffii (K. phaffii) created DAS1 and DAS2 gene knockout strains, which use the methanol pathway to generate more ATP and increase SAM production. These strains showed an increase in the flux of methanol oxidation and an increase in ATP production. The gene knockout retarded the cell growth in the late phase of induction, did not influence the total methanol consumption or the activity of SAM synthetase, but did significantly increase the yield of SAM by 43.7%. The metabolic flux of methanol oxidation is increased by DAS1 and DAS2 gene knockout, which leads to an increase in ATP and higher SAM production.

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“…The SAM2 gene from S. cerevisiae BY4741 was inserted into pRS425 using the TEF1 promoter and PGI1 terminator to yield pRS425-P TEF1 -SAM2 -T PGI1 . The genes noxE (from Lactococcus lactis ), vhb (encoding Vitreoscilla hemoglobin), and ptxD ( S. cerevisiae codon-optimized from Pseudomonas stutzeri ) were assembled with HXT7 promoter and HXT7 terminator into YCPlac33 to yield the recombinant plasmids YCplac33-P HXT7 - noxE -T HXT7 , YCplac33-P HXT7 - vhb -T HXT7 , and YCplac33-P HXT7 - ptxD -T HXT7 , respectively (Figure ). The recombinant vectors were transformed into E. coli Trans 10 by a heat shock at 42 °C for 30 s. The expression vector was transformed into yeast S. cerevisiae BY4741-MH using the LiAc/SS carrier DNA/PEG method.…”

Section: Materials and Methodsmentioning

confidence: 99%

Enhanced S-Adenosylmethionine Production by Increasing ATP Levels in Baker’s Yeast (Saccharomyces cerevisiae)

Chen

Tan

2018

J. Agric. Food Chem.

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In the biosynthesis of S-adenosylmethionine (SAM) in baker's yeast ( Saccharomyces cerevisiae), ATP functions as both a precursor and a driving force. However, few published reports have dealt with the control of ATP concentration using genetic design. In this study we have adopted a new ATP regulation strategy in yeast for enhancing SAM biosynthesis, including altering NADH availability and regulating the oxygen supply. Different ATP regulation systems were designed based on the introduction of water-forming NADH oxidase, Vitreoscilla hemoglobin, and phosphite dehydrogenase in combination with overexpression of the gene SAM2. Via application of this strategy, after 28 h cultivation, the SAM titer in the yeast strain ABYSM-2 reached a maximum level close to 55 mg/L, an increase of 67% compared to the control strain. The results show that the ATP regulation strategy is a valuable tool for SAM production and might further enhance the synthesis of other ATP-driven metabolites in yeast.

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Control of ATP concentration in Escherichia coli using an ATP-sensing riboswitch for enhanced S-adenosylmethionine production

Abstract: We proposed an ATP-sensing riboswitch-based strategy which could be applied to regulate the intracellular ATP concentration dynamically inE. coli. This strategy will be most beneficial for enhancing the production of the ATP-driven metabolites.

Cited by 18 publications

References 32 publications

Production of (2S)-sakuranetin from (2S)-naringenin in Escherichia coli by strengthening methylation process and cell resistance

Production of (2S)-sakuranetin from (2S)-naringenin in Escherichia coli by strengthening methylation process and cell resistance

Knockout of the DAS gene increases S-adenosylmethionine production in Komagataella phaffii

Enhanced S-Adenosylmethionine Production by Increasing ATP Levels in Baker’s Yeast (Saccharomyces cerevisiae)

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