Control of auxin-induced callus formation by bZIP59–LBD complex in Arabidopsis regeneration

Xu, Chongyi; Cao, Huifen; Zhang, Qianqian; Wang, Hongzhe; Xin, Wei; Xu, Enjun; Zhang, Shiqi; Yu, Ruixue; Yu, Dongxue; Hu, Yuxin

doi:10.1038/s41477-017-0095-4

Cited by 114 publications

(80 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The homo- and heterodimerization of LBD proteins are thought to be an important determinant of LR formation [29, 30]. The AtbZIP59 transcription factor has been reported to form complexes with LBDs to direct auxin-induced callus formation and LR development [31]. All these results suggest that homodimerization and heterodimerization of signaling components are important in LR formation.…”

Section: Discussionmentioning

confidence: 99%

PRH1 mediates ARF7-LBD dependent auxin signaling to regulate lateral root development inArabidopsis thaliana

Zhang

Tao

Sun

et al. 2019

Preprint

View full text Add to dashboard Cite

18The development of lateral roots in Arabidopsis thaliana is strongly dependent on 19 signaling directed by the AUXIN RESPONSE FACTOR7 (ARF7), which in turn 20 activates LATERAL ORGAN BOUNDARIES DOMAIN (LBD) transcription factors 21 (LBD16, 18, 29 and 33). Here, the product of PRH1, a PR-1 homolog annotated 22 previously as encoding a pathogen-responsive protein, was identified as a target of 23 ARF7-mediated auxin signaling and also as participating in the development of lateral 24 roots. PRH1 was shown to be strongly induced by auxin treatment, and plants lacking 25 a functional copy of PRH1 formed fewer lateral roots. The transcription of PRH1 was 26 controlled by the binding of both ARF7 and LBDs to its promoter region. An 27 interaction was detected between PRH1 and GATA23, a protein which regulates cell 28 identity in lateral root founder cells. 29 2 30 31 Author Summary 32 In Arabidopsis thaliana AUXIN RESPONSE FACTOR7 (ARF7)-mediated auxin 33 signaling plays a key role in lateral roots (LRs) development. The LATERAL ORGAN 34 BOUNDARIES DOMAIN (LBD) transcription factors (LBD16, 18, 29 and 33) act 35 downstream of ARF7-mediated auxin signaling to control LRs formation. Here, the 36 PR-1 homolog PRH1 was identified as a novel target of both ARF7 and LBDs 37 (especially the LBD29) during auxin induced LRs formation, as both ARF7 and 38LBDs were able to bind to the PRH1 promoter. More interestingly, PRH1 has a 39 physical interaction with GATA23, which has been also reported to be up-regulated 40 by auxin and influences LR formation through its regulation of LR founder cell 41 identity. Whether the interaction between GATA23 and PRH1 affects the stability 42 and/or the activity of either (or both) of these proteins remains an issue to be explored. 43 This study provides improves new insights about how auxin regulates lateral root 44 development. 3 45 Introduction 46 The architecture of the root system depends on the density of lateral roots (LRs) 47 formed along with the extent of root branching. LRs are initiated from mature 48 pericycle cells lying adjacent to the xylem pole, referred to in Arabidopsis thaliana as 49 the xylem pole pericycle [1, 2]. A subset of these cells, namely the founder cells, 50 undergo a series of highly organized divisions to form an LR primordium, which 51 eventually develops into an LR. The entire process of LR development, from its 52 initiation to its emergence, is regulated by auxin [1, 3, 4]. The accumulation of auxin 53 in protoxylem cells results in the priming of neighboring pericycle cells to gain 54 founder cell identity, forming an LR pre-branch site [5, 6]. This auxin signaling is 55 mediated by the proteins ARF7 and ARF19, which act in the IAA28-dependent 56 pathway [7, 8]. LR initiation depends on the transcriptional activation, through the 57 involvement of the transcription factor LBD16 and LBD18, of either E2Fa or CDK 58 A1;1 and CYC B1;1 [9, 10]. Other downstream targets such as EXP14 and EXP17 and 59 the products of certain cell wall loosening-related genes ...

show abstract

Section: Discussionmentioning

confidence: 99%

PRH1 mediates ARF7-LBD dependent auxin signaling to regulate lateral root development inArabidopsis thaliana

Zhang

Tao

Sun

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…MdLBD3, a gene homologous to AtLBD3/AtLBD9, is induced by exogenous cytokinin [38]. In rice (Oryza sativa L.), OsLBD3-1 is expressed only in roots, and OsLBD3-2 is expressed in roots, shoots, and petioles [39]. We found that a deletion on MdLBD3 promoter produced an additional MdWRKY2 binding motif and created a rise in MdLBD3 expression.…”

Section: Discussionmentioning

confidence: 81%

Development and validation of functional markers from Dw1 and Dw2 loci to accurately predict apple rootstock dwarfing ability

Yang

Shen²,

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: ‘M9’ is a widely used apple dwarfing rootstock due to the outstanding effects on both precocity and vigorous control. Two quantitative trait loci (QTLs), Dw1 and Dw2 , for the dwarfing effect were previously mapped on ‘M9’, but the genetic variations that underpin the dwarfing ability have not been elucidated to date. Result: By using ‘Red Fuji’ trees grafted on 1123 hybrids from ‘ M alus baccata ’ × ‘M9’, the intervals of Dw1 and Dw2 were narrowed down. MdLBD3 and MdARF6 were predicted as potential candidate genes from Dw1 , while MdG3OX3 was a possible candidate gene from Dw2 . An 11 bp deletion at -339 bp upstream of the transcription start site (TSS) of MdLBD3 generated a new cis-element binding site with MdWRKY2 and caused increased expression of MdLBD3 . Coincidently, a ten bp deletion at -278 bp upstream of the TSS of MdG3OX3 created an additional binding site of MdABI5 , leading to higher expression of MdG3OX3 . At -954 bp of the MdARF6 promoter, a 14 bp insertion destroyed the binding ability by MdABI5 and reduced MdARF6 expression. The genotype effects of these insertion and deletions as diagnostic markers on dwarfing traits (tree height, trunk diameter, and canopy width) were estimated in 108 F1 hybrids. The genomic predicted genetic values (GEGV) were calculated by adding up the genotype effects of the three markers and the population mean phenotype. The GEGV of the dwarfing traits exhibited high correlation coefficients of 0.93, 0.94, and 0.93 in terms of tree height, trunk diameter, and canopy width for the observed phenotype values, respectively. The predictability of GEGV was validated in 64 Malus accessions. Conclusion : The development of the three functional markers, Ld/Li, Ad/Ai, and Gd/Gi, ensures the accurate genomic assisted prediction of dwarfing ability in apple rootstock breeding. The data also suggested that ABA, auxin, GA, and zeatin signals may be involved in the regulation of apple rootstock dwarfing mechanism.

show abstract

“…In Triticum aestivum, regeneration e ciency was associated with auxin exposure time and catalase metabolism during somatic embryogenesis. Induction of pluripotent cells, termed, "callus," by auxin represents a typical cell fate change required for plant in vitro regeneration [84]. Studies have shown that relative signaling through MONOPTEROS (MP)/ARF 5 was required for shoot formation in Arabidopsis callus.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative Upland Cotton line (Gossypium hirsutum L.)

Parris

et al. 2020

Preprint

View full text Add to dashboard Cite

• Background • Genotype independent transformation and whole plant regeneration through somatic embryogenesis relies heavily on the intrinsic ability of a genotype to regenerate. • Results • In this study, gene expression profiles of a highly regenerable Gossypium hirsutum L. cultivar, Jin668, were analyzed at two critical developmental stages during somatic embryogenesis, non-embryogenic callus (NEC) cells and embryogenic callus (EC) cells. The rate of EC formation in Jin668 is 96%. Differential gene expression analysis revealed a total of 5,333 differentially expressed genes (DEG) with 2,534 upregulated and 2,799 downregulated in EC. A total of 144 genes were unique to NEC cells and 174 genes unique to EC. Clustering and enrichment analysis identified genes upregulated in EC that function as transcription factors/DNA binding, phytohormone response, oxidative reduction, and regulators of transcription; while genes categorized in methylation pathways were downregulated. Four key transcription factors were identified based on their sharp upregulation in EC tissue; LEAFY COTYLEDON 1 (LEC1), BABY BOOM (BBM), FUSCA (FUS3) and AGAMOUS-LIKE15 with distinguishable subgenome expression bias. • Conclusions • This comparative analysis of NEC and EC transcriptomes gives new insights into the genetic underpinnings of somatic embryogenesis in cotton.

show abstract

Control of auxin-induced callus formation by bZIP59–LBD complex in Arabidopsis regeneration

Cited by 114 publications

References 47 publications

PRH1 mediates ARF7-LBD dependent auxin signaling to regulate lateral root development inArabidopsis thaliana

PRH1 mediates ARF7-LBD dependent auxin signaling to regulate lateral root development inArabidopsis thaliana

Development and validation of functional markers from Dw1 and Dw2 loci to accurately predict apple rootstock dwarfing ability

Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative Upland Cotton line (Gossypium hirsutum L.)

Contact Info

Product

Resources

About