Control of calbindin‐D<sub>28K</sub> expression in developing mouse kidney

Davies, Jamie A.

doi:10.1002/aja.1001990105

Cited by 36 publications

(18 citation statements)

References 29 publications

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“…The Calb1 protein is well established as a marker of the developing ureteric bud and its derivatives in the embryonic mouse kidney as well as in developmental studies in vitro using mouse kidney explant cultures (Davies 1994;Cebrián et al 2004). The expression pattern shown here is in agreement with the published data, which demonstrated Calb1 protein expressed in the ureteric bud-derivatives from 12.5 dpc, and in the connecting tubule from 15.5 dpc (Shamley et al 1992;Cebrián et al 2004).…”

Section: Calb1 a Marker Of Ureteric Tip And Trunk (Collecting Duct) supporting

confidence: 94%

Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney

Georgas

Rumballe

Wilkinson

et al. 2008

Histochem Cell Biol

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The kidney is the most complex organ within the urogenital system. The adult mouse kidney contains in excess of 8,000 mature nephrons, each of which can be subdivided into a renal corpuscle and 14 distinct tubular segments. The histological complexity of this organ can make the clarification of the site of gene expression by in situ hybridisation difficult. We have defined a panel of seven antibodies capable of identifying the six stages of early nephron development, the tubular nephron segments and the components of the renal corpuscle within the embryonic and adult mouse kidney. We have analysed in detail the protein expression of Wt1, Calb1 Aqp1, Aqp2 and Umod using these antibodies. We have then coupled immunohistochemistry with RNA in situ hybridisation in order to precisely identify the expression pattern of different genes, including Wnt4, Umod and Spp1. This technique will be invaluable for examining at high resolution, the structure of both the developing and mature nephron where standard in situ hybridisation and histological techniques are insufficient. The use of this technique will enhance the expression analyses of genes which may be involved in nephron formation and the function of the mature nephron in the mouse.

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Section: Calb1 a Marker Of Ureteric Tip And Trunk (Collecting Duct) supporting

confidence: 94%

Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney

Georgas

Rumballe

Wilkinson

et al. 2008

Histochem Cell Biol

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“…Earlier immunohistochemical assays showed that P CD Amp1 (25) Tenascin (37) Wnt-11 (7) P CD Amp1 (6) Wnt-4 (7) Calbindin-D 28K (38) Laminin ␣1 (3) Hoxa11 (26) BMP-7 (9) Galectin-3 (46) Hoxd 11 (26) Pax-2 (39) Hyaluronan (47) Glypican-5 (27) LIF (10) Nephronectin (29) (6) is colocalized with osteopontin (45) in the extracellular matrix around the ampulla. Both proteins are strongly expressed at the ampullar tip while their expression decreases along the maturing CD.…”

Section: Resultsmentioning

confidence: 99%

SBA-Positive Fibers between the CD Ampulla, Mesenchyme, and Renal Capsule

Schumacher¹,

Strehl²,

Vries³

et al. 2002

Journal of the American Society of Nephrology

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Abstract. During kidney development, the CD shows two peculiarities. First, the tip of the CD ampulla is always found at a specific distance from the organ capsule. Second, the CD growth occurs as a perfectly straight elongation. It is unknown whether the CD-specific growth is dependent on hormonal action or on structural elements. Histochemical experiments on neonatal rabbit kidney yielded new insight into the interface of the CD ampulla and the surrounding nephrogenic mesenchyme. Incubation of tissue sections with soybean lectin (SBA) showed the existence of fibers extending in a radial course from the ampullar tip through the mesenchyme toward the organ capsule. SBA labeling did not colocalize with collagen type I, III, IV, V, and VI, laminin, fibronectin, and tenascin. It is assumed that while the kidney increases in volume the structural fixation of the ampullar tip by the SBA-positive fibers causes CD ampullae to maintain a constant distance from the organ capsule. The connection would explain the linear extension of the CD in relation to the organ capsule. In addition, the presented data suggest that the SBA-positive fibers between ampullar tip and organ capsule create a structural microcompartmentation of the nephrogenic zone.

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“…Previous reports on the ultrastructure of the ureteric bud tip have suggested that the basement membrane is incomplete, that cell-cell adhesion may be weakened there and that cells may even traffic between the ureteric bud and mesenchyme during normal development [53,54]. Using the relatively insensitive assay of staining for calbindin D-28K, an established marker of cells that maintain the differentiated state of the ureteric bud [7], we saw no evidence for cells leaving the bud in normal development or in cytochalasin D when development was inhibited with chlorate. Cells did stream out, however, when actively-developing ureteric bud tips were treated with the modest concentrations of cytochalasin D used in this report.…”

Section: Discussionmentioning

confidence: 99%

“…Day E10 urogenital ridges had nephric ducts but no sign of a ureteric bud, while day E11.5 kidneys had a T-shaped ureteric bud that had already branched once. Rudiments were cultured on isopore membrane filters (Millipore, Billerica, MA, USA) on Trowell screens [7]. The basic culture medium was MEM with nonessential amino acids (Sigma Chemical Co.), 10% newborn calf serum (Labtech, Ringmer, Sussex, UK), and penicillin/streptomycin (Sigma Chemical Co.).…”

Section: Organ Culturementioning

confidence: 99%

A role for microfilament-based contraction in branching morphogenesis of the ureteric bud

Michael

Sweeney

Davies

2005

Kidney International

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Control of calbindin‐D_28K expression in developing mouse kidney

Cited by 36 publications

References 29 publications

Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney

Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney

SBA-Positive Fibers between the CD Ampulla, Mesenchyme, and Renal Capsule

A role for microfilament-based contraction in branching morphogenesis of the ureteric bud

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Control of calbindin‐D28K expression in developing mouse kidney

Cited by 36 publications

References 29 publications

Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney

Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney

SBA-Positive Fibers between the CD Ampulla, Mesenchyme, and Renal Capsule

A role for microfilament-based contraction in branching morphogenesis of the ureteric bud

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Control of calbindin‐D_28K expression in developing mouse kidney