Control of cell adhesion and proliferation utilizing polysaccharide composite film scaffolds

Iijima, Kazutoshi; Tsuji, Yuna; Kuriki, Izumi; Kakimoto, Atsushi; Nikaido, Yuichi; Ninomiya, Rie; Iyoda, Takuya; Fukai, Fumio; Hashizume, Makoto

doi:10.1016/j.colsurfb.2017.09.025

Cited by 26 publications

(20 citation statements)

References 55 publications

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“…This indicated low adsorption of cell adhesion molecules contained in the culture media and that produced by cells on the surface of the fibers. We have previously demonstrated that NIH3T3 cells adhered to but did not spread on the CS/CHI composite films prepared by hot-pressing PIC gels and modification of the films with FN improved cellular adhesion …”

Section: Discussionmentioning

confidence: 99%

“…If the cells prefer the bottom surfaces of the cell culture plates in which the fibers are placed, cells will adhere to and spread on the cell culture plate. The other reason for low cell adhesion is due to the lower ability of CS/CHI for spreading of NIH3T3 cells, as we evaluated in a previous study . In this study, we used well-characterized CS/CHI PIC as the first attempt.…”

Section: Discussionmentioning

confidence: 99%

“…We have previously demonstrated that NIH3T3 cells adhered to but did not spread on the CS/CHI composite films prepared by hot-pressing PIC gels and modification of the films with FN improved cellular adhesion. 30 In order to improve the spreading of cells, loading of COL in the hollow fibers was examined. Cells adhere to COL through α1β1 and α2β1 integrins.…”

Section: ■ Discussionmentioning

confidence: 99%

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Preparation of Cell-Paved and -Incorporated Polysaccharide Hollow Fibers Using a Microfluidic Device

Iijima

Ichikawa

Ishikawa

et al. 2019

ACS Biomater. Sci. Eng.

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Cellular constructs having hollow tubular structures are expected to be used as artificial blood vessels. We have recently demonstrated that water-insoluble polyion complexes (PICs) were formed from water-soluble polysaccharides with opposite charges at the interface of coaxial flows, which resulted in the formation of hollow fibers. In this study, both inside- and outside-cell-laden chondroitin sulfate C (CS)/chitosan (CHI) hollow fibers were prepared by utilizing a microfluidic device and modification with cell adhesive molecules. Loading of type I collagen (COL) and surface modification with fibronectin and gelatin using layer-by-layer assembly techniques improved the adhesion and spreading of fibroblast cells to/on the surface of CS/CHI hollow fibers. On the other hand, by suspending mesenchymal stem cells (MSCs) in the core flow solution, cells were successfully loaded in the walls of the hollow fibers. As the culture time extended, cells trapped in the PIC structures constituting the wall of the hollow fibers migrated to the interface between the hollow fibers and the medium: cells adhered to and stretched “on” the lumen surfaces in the COL-loaded fibers. In contrast, for the case of unmodified hollow fibers, it was difficult for cells to adhere to the lumen surfaces. Therefore, cell aggregates were formed “in” the lumen. Results of the live/dead assay and MTT assay clearly demonstrated that MSCs possessed certain levels of cell viability and proliferated for up to 10 days, especially for the cases of COL-loaded hollow fibers. On the basis of these results, the utility of the present hollow fibers in the formation of cellular constructs corresponding to blood vessels is also discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

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Preparation of Cell-Paved and -Incorporated Polysaccharide Hollow Fibers Using a Microfluidic Device

Iijima

Ichikawa

Ishikawa

et al. 2019

ACS Biomater. Sci. Eng.

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“…In the hot-press technique, gel-like PICs are hot-pressed and formed into films [19,20]. By this method, films can be obtained within 30 min and are applicable as drug carriers [21] and cell scaffolds [22]. However, it is difficult to prepare films containing several anionic polysaccharides because of the differences in reactivity between cationic and anionic polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Characterization of Polysaccharide Composite Films from Polyion Complex Particles

Yamazaki

Iijima

2020

Polymers

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Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures. This study demonstrates that polysaccharide composite films can be fabricated from polyion complexes (PICs) with their particles used as building components. Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick. These films were homogenous and water insoluble. It was revealed that the component anionic polysaccharides affected the film’s properties such as the swelling behavior and mechanical characteristics. Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined. Both NIH3T3 and A549 cells adhered to heparin/chitosan (HEP/CHI) film because HEP has an affinity for integrin through fibronectin. However, A549 cells adhered to chondroitin sulfate (CS)/CHI and hyaluronic acid (HYA)/CHI films, whereas NIH3T3 cells did not, because both CS and HYA have affinity for CD44. These results indicated that the biological functions of anionic polysaccharides were maintained on the surface of the composite films. It was also possible to fabricate films composed of three kinds of polysaccharides: one cationic polysaccharide and two kinds of anionic polysaccharides. These results show that the properties of films composed of three kinds of polysaccharides may be controllable depending on the anionic polysaccharide composition rates.

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“…This effect arises from a combination of factors, including the type of PEs, the interactions between the chains inside the multilayer, and the properties of the last layer exposed at the interface. [205][206][207][208] The concept of stoichiometric PEMs introduced by Schlenoff et al suggests that multifilms have stoichiometric amounts of paired charged groups in the correct spatial proximity 209 to act as bulk zwitterions or "zwittersolids." 22 Cao and coworkers designed a versatile approach for surface modification using a combination of LbL and supramolecular host-guest interactions, which retained antifouling properties.…”

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confidence: 99%

Functionalized Polyelectrolytes for Bioengineered Interfaces and Biosensing Applications

Movilli

Huskens

2020

Organic Materials

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The possibility of tuning the chemical moieties and their density plays a fundamental role in targeting surface-confined molecular structures and their functionalities at macro and nanoscale levels. Such interfacial control is crucial for engineered coating formation and biorecognition purposes, where the type and density of ligands/receptors at the surface affect the overall binding affinities and the device performance. Together with the well-established selfassembled monolayers, a surface modification approach based on polyelectrolytes (PEs) has gained importance to provide desired characteristics at the substrate interface. This review presents the innovations of functional PEs, modified in a preceding synthetic step, and their wide applicability in functional (a)biotic substrates. Examples of 2D and 3D architectures made by modified PEs are reviewed in relation with the reactive groups grafted to the PE backbones. The main focus lies on the strategy to use modified PEs to form bioengineered coatings for orthogonally anchoring biological entities, manufacturing biocidal/antifouling films, and their combinations in functional biosensing applications. Figure 4 (a) PLL grafted with PEG, PMOXA, PEOXA, and PMOZI antifouling moieties, and (b) the residual adsorbed dry thickness from human serum. Reprinted and adapted with permission from Ref. 153.Figure 11 Efficiency of ligand-specific internalization by macrophages for differently functionalized interfaces of PLGA-covered particles. Type of modified PEs used, from left to right: PLL-PEG, PLL-PEG-RGD, and PLL-PEG-RDG (nonbinding). Adapted with permission from Ref. 266.

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Control of cell adhesion and proliferation utilizing polysaccharide composite film scaffolds

Cited by 26 publications

References 55 publications

Preparation of Cell-Paved and -Incorporated Polysaccharide Hollow Fibers Using a Microfluidic Device

Preparation of Cell-Paved and -Incorporated Polysaccharide Hollow Fibers Using a Microfluidic Device

Fabrication and Characterization of Polysaccharide Composite Films from Polyion Complex Particles

Functionalized Polyelectrolytes for Bioengineered Interfaces and Biosensing Applications

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