Control of cell nucleus shapes via micropillar patterns

Zhang, Pan; Yan, Changhao; Peng, Rong; Zhao, Yingchun; He, Yang; Ding, Jiandong

doi:10.1016/j.biomaterials.2011.11.023

Cited by 121 publications

(111 citation statements)

References 43 publications

Supporting

Mentioning

106

Contrasting

Order By: Relevance

“…[34][35][36][37][38] The improved bioactivity of nHA coated PLGA surfaces in the present study may be attributed to the following: first, the modified surfaces are rough and contain a lot of calcium and phosphorus, which are biomimetic and helpful for cell adhesion, migration, proliferation, and bone matrix formation. In bone tissue engineering, the BMSCs defined as seed cells, can disintegrate the skeleton of the scaffolds by secreting proteases in the early stages of bone remodeling.…”

Section: Discussionmentioning

confidence: 77%

Enhancing the bioactivity of Poly(lactic-co-glycolic acid) scaffold with a nano-hydroxyapatite coating for the treatment of segmental bone defect in a rabbit model

Wang

He²,

Bi³

et al. 2013

IJN

Self Cite

View full text Add to dashboard Cite

Purpose: Poly(lactic-co-glycolic acid) (PLGA) is excellent as a scaffolding matrix due to feasibility of processing and tunable biodegradability, yet the virgin scaffolds lack osteoconduction and osteoinduction. In this study, nano-hydroxyapatite (nHA) was coated on the interior surfaces of PLGA scaffolds in order to facilitate in vivo bone defect restoration using biomimetic ceramics while keeping the polyester skeleton of the scaffolds. Methods: PLGA porous scaffolds were prepared and surface modification was carried out by incubation in modified simulated body fluids. The nHA coated PLGA scaffolds were compared to the virgin PLGA scaffolds both in vitro and in vivo. Viability and proliferation rate of bone marrow stromal cells of rabbits were examined. The constructs of scaffolds and autogenous bone marrow stromal cells were implanted into the segmental bone defect in the rabbit model, and the bone regeneration effects were observed. Results: In contrast to the relative smooth pore surface of the virgin PLGA scaffold, a biomimetic hierarchical nanostructure was found on the surface of the interior pores of the nHA coated PLGA scaffolds by scanning electron microscopy. Both the viability and proliferation rate of the cells seeded in nHA coated PLGA scaffolds were higher than those in PLGA scaffolds. For bone defect repairing, the radius defects had, after 12 weeks implantation of nHA coated PLGA scaffolds, completely recuperated with significantly better bone formation than in the group of virgin PLGA scaffolds, as shown by X-ray, Micro-computerized tomography and histological examinations. Conclusion: nHA coating on the interior pore surfaces can significantly improve the bioactivity of PLGA porous scaffolds.

show abstract

Section: Discussionmentioning

confidence: 77%

Enhancing the bioactivity of Poly(lactic-co-glycolic acid) scaffold with a nano-hydroxyapatite coating for the treatment of segmental bone defect in a rabbit model

Wang

He²,

Bi³

et al. 2013

IJN

Self Cite

View full text Add to dashboard Cite

show abstract

“…We observe that the correlation between cell morphology and nucleus morphology breaks down when the nuclei are directly affected by the surface design due to tight space constraints for the cells. This is supported by recent findings in which serious deformation of cell nuclei has been found on micropillar arrays with appropriate parameters, resulting in some very interesting cell nuclei shapes, such as squares, crosses, dumbbells and asymmetrically protruded spheres [41]. Enriching these morphological descriptions with either 3-D or dynamic (over time) measurements might be an interesting avenue.…”

Section: Biological Implicationsmentioning

confidence: 73%

Analysis of high-throughput screening reveals the effect of surface topographies on cellular morphology

et al. 2015

View full text Add to dashboard Cite

Surface topographies of materials considerably impact cellular behavior as they have been shown to affect cell growth, provide cell guidance, and even induce cell differentiation. Consequently, for successful application in tissue engineering, the contact interface of biomaterials needs to be optimized to induce the required cell behavior. However, a rational design of biomaterial surfaces is severely hampered because knowledge is lacking on the underlying biological mechanisms. Therefore, we previously developed a high-throughput screening device (TopoChip) that measures cell responses to large libraries of parameterized topographical material surfaces. Here, we introduce a computational analysis of high-throughput materiome data to capture the relationship between the surface topographies of materials and cellular morphology. We apply robust statistical techniques to find surface topographies that best promote a certain specified cellular response. By augmenting surface screening with data-driven modeling, we determine which properties of the surface topographies influence the morphological properties of the cells. With this information, we build models that predict the cellular response to surface topographies that have not yet been measured. We analyze cellular morphology on 2176 surfaces, and find that the surface topography significantly affects various cellular properties, including the roundness and size of the nucleus, as well as the perimeter and orientation of the cells. Our learned models capture and accurately predict these relationships and reveal a spectrum of topographies that induce various levels of cellular morphologies. Taken together, this novel approach of high-throughput screening of materials and subsequent analysis opens up possibilities for a rational design of biomaterial surfaces.

show abstract

“…Besides chemical modification, surface topography and even material stiffness have been realized to influence basic behaviours of cells including stem cells [85][86][87][88]. Three-dimensional cell behaviour might be also different from on two-dimensional substrates.…”

Section: Summary and Perspectivesmentioning

confidence: 99%

Poly(lactide- co -glycolide) porous scaffolds for tissue engineering and regenerative medicine

2012

Self Cite

View full text Add to dashboard Cite

Porous scaffolds fabricated from biocompatible and biodegradable polymers play vital roles in tissue engineering and regenerative medicine. Among various scaffold matrix materials, poly(lactide-co-glycolide) (PLGA) is a very popular and an important biodegradable polyester owing to its tunable degradation rates, good mechanical properties and processibility, etc. This review highlights the progress on PLGA scaffolds. In the latest decade, some facile fabrication approaches at room temperature were put forward; more appropriate pore structures were designed and achieved; the mechanical properties were investigated both for dry and wet scaffolds; a long time biodegradation of the PLGA scaffold was observed and a three-stage model was established; even the effects of pore size and porosity on in vitro biodegradation were revealed; the PLGA scaffolds have also been implanted into animals, and some tissues have been regenerated in vivo after loading cells including stem cells.

show abstract

Control of cell nucleus shapes via micropillar patterns

Cited by 121 publications

References 43 publications

Enhancing the bioactivity of Poly(lactic-co-glycolic acid) scaffold with a nano-hydroxyapatite coating for the treatment of segmental bone defect in a rabbit model

Enhancing the bioactivity of Poly(lactic-co-glycolic acid) scaffold with a nano-hydroxyapatite coating for the treatment of segmental bone defect in a rabbit model

Analysis of high-throughput screening reveals the effect of surface topographies on cellular morphology

Poly(lactide- co -glycolide) porous scaffolds for tissue engineering and regenerative medicine

Contact Info

Product

Resources

About