Control of cyst nematodes by Lysobacter enzymogenes strain C3 and the role of the antibiotic HSAF in the biological control activity

Yuen, Gary Y.; Broderick, Kyle C.; Jochum, C. C.; Chen, Carl J.; Caswell-Chen, E. P.

doi:10.1016/j.biocontrol.2017.11.007

Cited by 15 publications

(11 citation statements)

References 23 publications

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“…There has been considerable progress in the use and development of rhizosphere microorganisms as a means to manage PPN . Martinuz et al .…”

Section: Discussionmentioning

confidence: 99%

“…There has been considerable progress in the use and development of rhizosphere microorganisms as a means to manage PPN. 43,44 Martinuz et al found that treatment of tomatoes with Rhizobium etli G12 reduced the penetration, development and reproduction of Meloidogyne incognita. 45 In this study, we selected a novel biocontrol agent, Sneb159, out of 804 bacterial strains screened in a field heavily infested with H. glycines.…”

Section: Discussionmentioning

confidence: 99%

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Biocontrol potential ofMicrobacterium maritypicumSneb159 againstHeterodera glycines

Zhao

Dan

Wang

et al. 2019

Pest Management Science

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BACKGROUND The soybean cyst nematode Heterodera glycines (Ichinohe) is the most devastating pathogen affecting soybean production worldwide. Biocontrol agents have become eco‐friendly candidates to control pathogens. The aim of this study was to discover novel biocontrol agents against H. glycines. RESULTS Microbacterium maritypicum Sneb159, screened from 804 strains, effectively reduced the number of females in field experiments conducted in 2014 and 2015. The stability and efficiency of H. glycines control by Sneb159 was further assessed in growth chamber and field experiments. Sneb159 decreased H. glycines population densities, especially the number of females by 43.9%–67.7%. To confirm Sneb159 induced plant resistance, a split‐root assay was conducted. Sneb159 induced local and systemic resistance to suppress the penetration and development of H. glycines, and enhanced the gene expression of PR2, PR3b, and JAZ1, involved in the salicylic acid and jasmonic acid pathways. CONCLUSION This is the first report of M. maritypicum Sneb159 suppressing H. glycines infection. This effect may be the result of Sneb159‐induced resistance. Our study indicates that M. maritypicum Sneb159 is a promising biocontrol agent against H. glycines. © 2019 Society of Chemical Industry

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“…There has been considerable progress in the use and development of rhizosphere microorganisms as a means to manage PPN . Martinuz et al .…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Biocontrol potential ofMicrobacterium maritypicumSneb159 againstHeterodera glycines

Zhao

Dan

Wang

et al. 2019

Pest Management Science

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“…The destruction of the PKS-NRPS gene in strain C3 lost the capacity to produce HSAF and reduce the ability to restrain fungal growth ( Yu et al, 2007 ; Li et al, 2012 ). HSAF production was also proved to be a crucial mechanism to control cyst nematodes in L. enzymogenes C3 ( Yuen et al, 2018 ). The comparative genomic analysis showed that the genes and gene clusters involved in secondary metabolite synthesis were significantly different among the six Lysobacter strains.…”

Section: Discussionmentioning

confidence: 99%

“…The cyclic lipodepsipeptide HSAF derived from L. enzymogenes C3 inhibited the activity of fungal neuro phthalamide synthase and altered the composition of sphingolipids in the cell membrane, resulting in depolarization of the target fungal hyphae (hyphae expansion, numerous branches, etc.) and thus exhibited potent antifungal activity ( Kobayashi and Yuen, 2005 ; Yuen et al, 2018 ). L. enzymogenes OH11 could also produce HSAF, which showed significant antagonistic activities toward diverse pathogenic fungi, and was reported to be a highly effective and broad-spectrum antibiotic of microbial origin.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomics provides insights into the potential biocontrol mechanism of two Lysobacter enzymogenes strains with distinct antagonistic activities

Zhang

Xie

et al. 2022

Front. Microbiol.

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Lysobacter enzymogenes has been applied as an abundant beneficial microorganism to control plant disease; however, most L. enzymogenes strains have been mainly reported to control fungal diseases, not bacterial diseases. In this study, two L. enzymogenes strains were characterized, of which CX03 displayed a broad spectrum of antagonistic activities toward multiple bacteria, while CX06 exhibited a broad spectrum of antagonistic activities toward diverse fungi and oomycete, and the whole genomes of the two strains were sequenced and compared. The genome annotation showed that the CX03 genome comprised a 5,947,018 bp circular chromosome, while strain CX06 comprised a circular 6,206,196 bp chromosome. Phylogenetic analysis revealed that CX03 had a closer genetic relationship with L. enzymogenes ATCC29487T and M497-1, while CX06 was highly similar to L. enzymogenes C3. Functional gene annotation analyses of the two L. enzymogenes strains showed that many genes or gene clusters associated with the biosynthesis of different secondary metabolites were found in strains CX03 and CX06, which may be responsible for the different antagonistic activities against diverse plant pathogens. Moreover, comparative genomic analysis revealed the difference in bacterial secretory systems between L. enzymogenes strains CX03 and CX06. In addition, numerous conserved genes related to siderophore biosynthesis, quorum sensing, two-component systems, flagellar biosynthesis and chemotaxis were also identified in the genomes of strains CX03 and CX06. Most reported L. enzymogenes strains were proven mainly to suppress fungi, while CX03 exhibited direct inhibitory activities toward plant bacterial pathogens and showed an obvious role in managing bacterial disease. This study provides a novel understanding of the biocontrol mechanisms of L. enzymogenes, and reveals great potential for its application in plant disease control.

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“…belonging to the Xanthomonadaceae family, are commonly found in the plant rhizosphere where they control phytopathogenic microorganisms [15]. This ability mainly relies on the release of lytic enzymes and antibiotics like the Heat Stable Antifungal Factor (HSAF), which are toxic to phytopathogenic (micro)organisms [16, 17]. In L. enzymogenes DSFs, DFs and IND regulate HSAF biosynthesis [9, 18, 19] and twitching motility [20].…”

Section: Introductionmentioning

confidence: 99%

Listening to bacterial Esperanto: transcriptome reprogramming in a plant beneficial rhizobacterium

Bejarano

Perazzolli

Pertot

et al. 2021

Preprint

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Intraspecies, interspecies and interkingdom signalling occurring via diffusible communication signals (DCSs) continuously shapes the gene expression patterns of individual bacterial species in the rhizosphere, affecting bacterial functions within the rhizosphere microbial community. To unravel how DCSs influence rhizosphere competence of plant beneficial rhizobacteria, we carried out a functional and transcriptome analysis on the plant beneficial bacterium Lysobacter capsici AZ78 (AZ78). Results reveal that 13-methyltetradecanoic acid and indole, glyoxylic acid and 2,3-butanedione play a relevant role in the interaction between L. capsici members and other soil-living (micro)organisms. DCSs regulated mechanisms of multistress and multidrug tolerance and persistence, including detoxification, motility, antibiotic production, and expression of secretion systems. In particular, 13-methyltetradecanoic acid, glyoxylic acid and 2,3-butanedione might enable AZ78 to rapidly colonize the rhizosphere. Moreover, glyoxylic acid and 2,3-butanedione elicit biological responses to outcompete other (micro)organisms. In contrast, indole inactivates twitching motility and antibiotic production. These results demonstrate that DCSs influence the functioning of plant beneficial rhizobacteria and suggest that plant beneficial L. capsici strains could use them to foster rhizosphere colonization and enhance in vivo activities to increase soil health and plant fitness.

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Control of cyst nematodes by Lysobacter enzymogenes strain C3 and the role of the antibiotic HSAF in the biological control activity

Cited by 15 publications

References 23 publications

Biocontrol potential ofMicrobacterium maritypicumSneb159 againstHeterodera glycines

Biocontrol potential ofMicrobacterium maritypicumSneb159 againstHeterodera glycines

Comparative genomics provides insights into the potential biocontrol mechanism of two Lysobacter enzymogenes strains with distinct antagonistic activities

Listening to bacterial Esperanto: transcriptome reprogramming in a plant beneficial rhizobacterium

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