Control of Drosophila wing and haltere development by the nuclear vestigial gene product.

Williams, Jim A.; Bell, John B.; Carroll, Sean B.

doi:10.1101/gad.5.12b.2481

Cited by 267 publications

(227 citation statements)

References 27 publications

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“…40,41 In addition, the fact that vg is highly expressed in the same area and is absolutely required for wing pouch development, led us to monitor cell death regulators expression in both wild-type and vg null / þ discs. 7 Using aminopterin, we showed that VG and DHFR are required for the integrity of wing margin cells, in addition to cell proliferation. A decrease in both products, induces specific rpr expression, DIAP1 downregulation and caspase-mediated cell death at the D/V boundary of the wing disc.…”

Section: Discussionmentioning

confidence: 98%

“…Therefore, the complete loss of wing tissue in vg null homozygous mutant 7,11 and the impossibility to recover vg null clones 4,36 are probably due to both proliferation impairment and apoptosis triggering. The requirement for DHFR during both processes raises the possibility of mutual cross-talk between cell survival and proliferation that would rely on VG function and DNA synthesis.…”

Section: Discussionmentioning

confidence: 99%

“…[4][5][6] The vg gene encodes a nuclear protein expressed during embryogenesis in the presumptive regions of wing and halter imaginal discs in the central nervous system and in a subset of thoracic muscles. 7 All vg mutants are characterized by a reduced wing phenotype, indicating a requirement for VG in wing development. During larval development vg expression in the wing disc is dependent on the sequential activation of two intronic enhancers.…”

Section: Introductionmentioning

confidence: 99%

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The Drosophila wing differentiation factor Vestigial–Scalloped is required for cell proliferation and cell survival at the dorso-ventral boundary of the wing imaginal disc

et al. 2003

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Links between genes involved in development, proliferation and apoptosis have been difficult to establish. In the Drosophila wing disc, the vestigial (vg) and the scalloped (sd) gene products dimerize to form a functional transcription factor. Ectopic expression of vg in other imaginal discs induces outgrowth and wing tissue specification. We investigated the role of the VG-SD dimer in proliferation and showed that vg antagonizes the effect of dacapo, the cyclin-cdk inhibitor. Moreover, ectopic vg drives cell cycle progression and in HeLa cultured cells, the VG-SD dimer induces cell proliferation per se. In Drosophila, ectopic vg induces expression of dE2F1 and its targets dRNR2 and string. In addition vg, but not dE2F1, interacts with and induces expression of dihydrofolate reductase (DHFR). Moreover, a decrease in VG or addition of aminopterin, a specific DHFR inhibitor, shift the dorso-ventral boundary cells of the disc to a cell death sensitive state that is correlated with reaper induction and DIAP1 downregulation. This indicates that vg in interaction with dE2F1 and DHFR is a critical player for both cell proliferation and cell survival in the presumptive wing margin area.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Drosophila wing differentiation factor Vestigial–Scalloped is required for cell proliferation and cell survival at the dorso-ventral boundary of the wing imaginal disc

et al. 2003

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“…The endogenous expression patterns of wg and dpp in the wing disc during the second and early third larval instars. vg (cross-hatched) is expressed where wg and dpp signals intersect and is required for proper wing and haltere development (Williams, Bell, & Carroll, 1991).…”

Section: Fig 2 Transdetermination Events In Drosophila Imaginal Discsmentioning

confidence: 99%

Transdetermination: Drosophila imaginal disc cells exhibit stem cell-like potency

McClure

Schubiger

2007

The International Journal of Biochemistry & Cell Biology

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Drosophila imaginal discs, the primordia of the adult fly appendages, are an excellent system for studying developmental plasticity. Cells in the imaginal discs are determined for their disc-specific fate (wingness, legness) during embryogenesis. Disc cells maintain their determination during larval development, a time of extensive growth and proliferation. Only when prompted to regenerate do disc cells exhibit lability in their determined identity. Regeneration in the disc is mediated by a localized region of cell division, known as the regeneration blastema. Most regenerating disc cells strictly adhere to their disc-specific identity; some cells however, switch fate in a phenomenon known as transdetermination. Similar regeneration and transdetermination events can be induced in situ by misexpression of the signaling molecule wingless. Recent studies indicate that the plasticity of disc cells during regeneration is associated with high morphogen activity and the reorganization of chromatin structure. Here we provide both a historical perspective of imaginal disc transdetermination, as well as discuss recent findings on how imaginal disc cells acquire developmental plasticity and multipotency. We also highlight how an understanding of imaginal disc transdetermination can enhance an understanding of developmental potency exhibited by stem cells.

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“…These indirectly activate decapentaplegic between the two compartments, establishing a gradient moving out from a central stripe in the wing [16]. Similarly, dorsal and ventral wing surfaces are defined by expression of apterous and vestigial, respectively, in the second instar [17,18]. This is just part of a complex network of interacting genes that establishes spatial information.…”

Section: Early Wing Development In the Flymentioning

confidence: 99%

Waiting in the wings: what can we learn about gene co-option from the diversification of butterfly wing patterns?

Jiggins¹,

Wallbank²,

Hanly³

2017

Phil. Trans. R. Soc. B

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One contribution of 17 to a theme issue 'Evo-devo in the genomics era, and the origins of morphological diversity'. A major challenge is to understand how conserved gene regulatory networks control the wonderful diversity of form that we see among animals and plants. Butterfly wing patterns are an excellent example of this diversity. Butterfly wings form as imaginal discs in the caterpillar and are constructed by a gene regulatory network, much of which is conserved across the holometabolous insects. Recent work in Heliconius butterflies takes advantage of genomic approaches and offers insights into how the diversification of wing patterns is overlaid onto this conserved network. WntA is a patterning morphogen that alters spatial information in the wing. Optix is a transcription factor that acts later in development to paint specific wing regions red. Both of these loci fit the paradigm of conserved protein-coding loci with diverse regulatory elements and developmental roles that have taken on novel derived functions in patterning wings. These discoveries offer insights into the 'Nymphalid Ground Plan', which offers a unifying hypothesis for pattern formation across nymphalid butterflies. These loci also represent 'hotspots' for morphological change that have been targeted repeatedly during evolution. Both convergent and divergent evolution of a great diversity of patterns is controlled by complex alleles at just a few genes. We suggest that evolutionary change has become focused on one or a few genetic loci for two reasons. First, pre-existing complex cis-regulatory loci that already interact with potentially relevant transcription factors are more likely to acquire novel functions in wing patterning. Second, the shape of wing regulatory networks may constrain evolutionary change to one or a few loci. Overall, genomic approaches that have identified wing patterning loci in these butterflies offer broad insight into how gene regulatory networks evolve to produce diversity.This article is part of the themed issue 'Evo-devo in the genomics era, and the origins of morphological diversity'.

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Control of Drosophila wing and haltere development by the nuclear vestigial gene product.

Cited by 267 publications

References 27 publications

The Drosophila wing differentiation factor Vestigial–Scalloped is required for cell proliferation and cell survival at the dorso-ventral boundary of the wing imaginal disc

The Drosophila wing differentiation factor Vestigial–Scalloped is required for cell proliferation and cell survival at the dorso-ventral boundary of the wing imaginal disc

Transdetermination: Drosophila imaginal disc cells exhibit stem cell-like potency

Waiting in the wings: what can we learn about gene co-option from the diversification of butterfly wing patterns?

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