Control of H-2 antigen and beta 2-microglobulin gene expression in mouse trophoblast cell clones.

Tanaka, Kenichi; Ozato, Keiko; Jay, Gilbert; Parnes, Jane R.; Ramanathan, Lata; Seidman, Jonathan G.; Chang, Kenneth S. S.; Appella, Ettore

doi:10.1073/pnas.80.18.5597

Cited by 9 publications

(1 citation statement)

References 35 publications

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“…Furthermore, the T3b transfectants were recognized by conventional TL typing sera for TL specificity (26) . The C57BL/6 trophoblast cell line (27) was obtained from Dr. Keiko Ozato (National Institute of Child Health and Human Development, Bethesda, MD) .…”

Section: Methodsmentioning

confidence: 99%

Highly restricted expression of the thymus leukemia antigens on intestinal epithelial cells.

Kaer

Itohara

et al. 1991

The Journal of Experimental Medicine

108

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SummaryThe TL region of the major histocompatibility complex ofthe mouse contains dozens of tandemly arranged class I genes, including those encoding the thymus leukemia (TL) antigens. TL antigens have been thought to be expressed only on the surface of some T lineage cells, namely immature thymocytes of some mouse strains (TL' strains), some leukemia cells, and activated T cells. While the function of TL antigens is unknown, recent studies have implicated the products of at least some TL region class I genes as molecules that present antigens to y/b T cells. Since some y/b T cells are known to be specifically associated with certain epithelial tissues, we have investigated the expression of some TL region class I genes in a variety of epithelium-containing tissues . Our results show that the TL antigen gene of C57BL/6 mice, T3b, and the TL antigen genes of BALB/c mice, T3d (previously T3°) and n8d (previously T13°), are highly expressed in the epithelium of the small intestine . In the case of T3b, we further show, using a T3 product-specific antibody, that its product is expressed on the surface of the columnar epithelial cells. In addition, we demonstrated that two other TL region class I genes of C57BL/6 origin, T9b and T21b, are also expressed nearly exclusively in intestinal epithelial cells . These results are consistent with the hypothesis that the products of these TL region class I genes are recognized by y/b T cell receptors of intestinal intraepithelial lymphocytes, a subset of -y/b T cells that is localized in the intestinal epithelium and has a restricted Vy repertoire. Finally, our study indicates that the relative levels of expression of the two homologous TL antigen genes, T3d and T18d, differ widely between the thymus and the intestine.

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Section: Methodsmentioning

confidence: 99%

Highly restricted expression of the thymus leukemia antigens on intestinal epithelial cells.

Kaer

Itohara

et al. 1991

The Journal of Experimental Medicine

108

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Differential Hm antigen expression on EC cells and early differentiated derivatives.

Simmler¹,

Avner²

1985

The EMBO Journal

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Differences in the expression of minor histocompatibility (Hm) alloantigens on two mouse embryonal carcinoma (EC) cell lines and the PYS‐2 and T.D.M.‐1 differentiated derivatives have been demonstrated by their ability to elicit a cytolytic T‐lymphocyte (CTL) response. Experiments involving the use of various responder‐target strain combinations on the one hand and Recombinant Inbred (RI) mice strains on the other have shown that: (i) there are major differences in Hm expression on the EC cells compared with the differentiated derivatives whose Hm expression appears more akin to that of adult splenocytes; (ii) although both EC cell lines show reduced Hm immunogenicity compared with adult splenocytes, major differences in the expression and possibly presentation between the F9 and PCC3 EC cell lines can be detected both by in vivo priming and by in vitro cold competition target experiments. These results are discussed in connection with the unexpected finding that some EC cell lines are capable of specific competition effects for appropriate CTL effectors despite their inability to stimulate such effectors in vitro and the absence of major histocompatibility complex (MHC) products.

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Trophoblast Cell Differentiation: Expression of the Placental Prolactin Family

et al. 1993

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Control of H-2 antigen and beta 2-microglobulin gene expression in mouse trophoblast cell clones.

Cited by 9 publications

References 35 publications

Highly restricted expression of the thymus leukemia antigens on intestinal epithelial cells.

Highly restricted expression of the thymus leukemia antigens on intestinal epithelial cells.

Differential Hm antigen expression on EC cells and early differentiated derivatives.

Trophoblast Cell Differentiation: Expression of the Placental Prolactin Family

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