Control of Hair Cell Excitability by Vestibular Primary Sensory Neurons

Brugeaud, Aurore; Travo, Cécile; Demêmes, Danielle; Lenoir, Marc; Llorens, Jordi; Puel, Jean–Luc; Chabbert, Christian

doi:10.1523/jneurosci.5185-06.2007

Cited by 48 publications

(61 citation statements)

References 45 publications

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“…without apparent damages of the neuron. The addition of TTX at 1 μM, a specific inhibitor of the voltage-gated sodium channels [26], abolished the spike-like component of the laser evoked membrane depolarization, as shown in Fig. 2(a) (n = 3).…”

Section: Ir Laser Pulses Elicit Action Potential In Drg Neuronsmentioning

confidence: 68%

Millisecond infrared laser pulses depolarize and elicit action potentials on in-vitro dorsal root ganglion neurons

Paris¹,

Marc²,

Charlot³

et al. 2017

Biomed. Opt. Express

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This work focuses on the optical stimulation of dorsal root ganglion (DRG) neurons through infrared laser light stimulation. We show that a few millisecond laser pulse at 1875 nm induces a membrane depolarization, which was observed by the patch-clamp technique. This stimulation led to action potentials firing on a minority of neurons beyond an energy threshold. A depolarization without action potential was observed for the majority of DRG neurons, even beyond the action potential energy threshold. The use of ruthenium red, a thermal channel blocker, stops the action potential generation, but has no effects on membrane depolarization. Local temperature measurements reveal that the depolarization amplitude is sensitive to the amplitude of the temperature rise as well as to the time rate of change of temperature, but in a way which may not fully follow a photothermal capacitive mechanism, suggesting that more complex mechanisms are involved.

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Section: Ir Laser Pulses Elicit Action Potential In Drg Neuronsmentioning

confidence: 68%

Millisecond infrared laser pulses depolarize and elicit action potentials on in-vitro dorsal root ganglion neurons

Paris¹,

Marc²,

Charlot³

et al. 2017

Biomed. Opt. Express

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show abstract

“…To determine the type of ionic channels involved in the generation of RGC and VGC responses, we used several pharmacological reagents. Bath application of TTX (10 M), a specific blocker of the voltagegated sodium channels (Brugeaud et al 2007), reversibly abolished the spikelike component of the LEVV in the two cell types (n ϭ 4 RGCs and n ϭ 7 VGCs; Fig. 2A).…”

Section: Mid-ir Laser Stimulations Trigger Transient Membrane Potentimentioning

confidence: 94%

TRPV4 channels mediate the infrared laser-evoked response in sensory neurons

Albert

Bec

Desmadryl

et al. 2012

Journal of Neurophysiology

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212

216

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“…Behavioral Experiments-The vestibular rating score was estimated as described previously (14,15). Tmprss3…”

Section: Assessment Of Balance Functionmentioning

confidence: 99%

Tmprss3, a Transmembrane Serine Protease Deficient in Human DFNB8/10 Deafness, Is Critical for Cochlear Hair Cell Survival at the Onset of Hearing

Fasquelle

Scott

Lenoir

et al. 2011

Journal of Biological Chemistry

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Mutations in the type II transmembrane serine protease 3 (TMPRSS3) gene cause non-syndromic autosomal recessive deafness (DFNB8/10), characterized by congenital or childhood onset bilateral profound hearing loss. In order to explore the physiopathology of TMPRSS3 related deafness, we have generated an ethyl-nitrosourea-induced mutant mouse carrying a protein-truncating nonsense mutation in Tmprss3 (Y260X) and characterized the functional and histological consequences of Tmprss3 deficiency. Auditory brainstem response revealed that wild type and heterozygous mice have normal hearing thresholds up to 5 months of age, whereas Tmprss3 Y260X homozygous mutant mice exhibit severe deafness. Histological examination showed degeneration of the organ of Corti in adult mutant mice. Cochlear hair cell degeneration starts at the onset of hearing, postnatal day 12, in the basal turn and progresses very rapidly toward the apex, reaching completion within 2 days. Given that auditory and vestibular deficits often co-exist, we evaluated the balancing abilities of Tmprss3 Y260X mice by using rotating rod and vestibular behavioral tests. Tmprss3 Y260X mice effectively displayed mild vestibular syndrome that correlated histologically with a slow degeneration of saccular hair cells. In situ hybridization in the developing inner ear showed that Tmprss3 mRNA is localized in sensory hair cells in the cochlea and the vestibule. Our results show that Tmprss3 acts as a permissive factor for cochlear hair cells survival and activation at the onset of hearing and is required for saccular hair cell survival. This mouse model will certainly help to decipher the molecular mechanisms underlying DFNB8/10 deafness and cochlear function.

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Control of Hair Cell Excitability by Vestibular Primary Sensory Neurons

Cited by 48 publications

References 45 publications

Millisecond infrared laser pulses depolarize and elicit action potentials on in-vitro dorsal root ganglion neurons

Millisecond infrared laser pulses depolarize and elicit action potentials on in-vitro dorsal root ganglion neurons

TRPV4 channels mediate the infrared laser-evoked response in sensory neurons

Tmprss3, a Transmembrane Serine Protease Deficient in Human DFNB8/10 Deafness, Is Critical for Cochlear Hair Cell Survival at the Onset of Hearing

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