Control of hyperglycemia significantly improves oxidative stress profile of pancreatic islets

Modak, Manisha; Parab, Pradip B.; Ghaskadbi, Surendra

doi:10.4161/isl.3.5.15941

Cited by 9 publications

(6 citation statements)

References 52 publications

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“…However, the aqueous extracts of Phragmites australis treated rats showed a significant reduction in the levels of these enzymes when compared to the diabetic group, thus an indication of the protective effects of the aqueous extracts over alloxan-induced liver damage which is in agreement with the other investigation [36]. Pancreatic Islets are especially vulnerable to oxidative stress mediated injuries because the antioxidant defence system of the pancreas is considerably weaker than those of other tissues [37]. There is a complex interaction between antioxidants and oxidants such as reactive oxygen species, which modulates the generation of oxidative stress [38].…”

Section: Discussionsupporting

confidence: 79%

Effect of Extracts Aqueous of Phragmites Australis on Carbohydrate Metabolism, Some Enzyme Activities and Pancreatic Islet Tissue in Alloxan-Induced Diabetic Rats

Derouiche

Manel

Hamida

2017

Int J Pharm Pharm Sci

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Objective: The present study was aimed to investigate the effects of the aqueous extract of rhizome of phragmites australis on carbohydrate metabolism, some enzyme activities and pancreatic tissue in alloxan induced diabetic rats. Methods:Wistar rats were divided into three groups (n=6) as control, diabetic group and test groups (Diabetic+AEPA). Diabetes in rats was induced by alloxane using a single peritoneal injection of 150 mg/kg dose. Aqueous extract of Phragmites australis was supplemented (200 mg/kg b. w) orally for three weeks. The aqueous extract of Phragmites australis was prepared and phytochemical were analyzed by using standard methods. Blood glucose level, pancreas histology and various biochemical parameters were assessed. Results:The results of the phytochemical analysis of aqueous extract of Phragmites australis (AEPA) revealed the presence of tannin, terpenoids, glycosides and flavonoids. Compared with the control, a significant decrease in the body weight gain (p<0.01) and increase in food intake (p<0.001) were noticed in the diabetic group. The biochemical evaluation showed significantly higher values for glucose (p<0.001), lipid profile (p<0.05), transaminases (p<0.05), amylase and alkaline phosphatase (p<0.001) activities in diabetic group compared with the control. Histology of the pancreas showed congestion of vessels and focal area of necrosis in diabetic untreated rats. However, treatment with aqueous extract of Phragmites australis significantly normalized blood glucose, serum biochemical profile and pancreas histomorphology. Conclusion:It is suggested that aqueous extract of Phragmites australis exhibits a benefic effect in rat to a great extent in attenuating and restoring the damage sustained by diabetes.

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Section: Discussionsupporting

confidence: 79%

Effect of Extracts Aqueous of Phragmites Australis on Carbohydrate Metabolism, Some Enzyme Activities and Pancreatic Islet Tissue in Alloxan-Induced Diabetic Rats

Derouiche

Manel

Hamida

2017

Int J Pharm Pharm Sci

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“…Expression of inflammatory cytokines and adhesion molecules serves as the signal to recruit leukocytes to the site of the damage, which are the source of reactive oxygen species and nitric oxide. Pancreatic islets express low levels of antioxidant enzymes in comparison to other tissues and are thus more vulnerable to oxidative stress that diminishes islet functions [36]. Even though endocrine insufficiency in CP is well documented [37], and cytokines were generally shown to contribute to b-cell dysfunction [38], there is no prior information on pancreatic cytokine profiles in CP patients with and without diabetes.…”

Section: Cytokines and B-cell Dysfunction In Cpmentioning

confidence: 99%

β-Cell Dysfunction in Chronic Pancreatitis

et al. 2012

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Chronic pancreatitis (CP) is a progressive inflammatory disease characterized by irreversible destruction of pancreatic secretory parenchyma, fibrosis, exocrine atrophy, and endocrine insufficiency leading to diabetes. Secondary diabetes occurring in CP subsequent to destruction of pancreatic β-cells is distinct, since it involves β-cell dysfunction amidst an inflammatory milieu. Even though considerable knowledge is available on the pathophysiology and clinical management of CP, relatively much less is known about the molecular events leading to β-cell dysfunction. Investigators have demonstrated that altered morphology, reduced β-cell mass, and β-cell numbers result in endocrine insufficiency. However, recent reports and our observations suggest that β-cell dysfunction develops in the early stages of CP while clinical diabetes manifests later, when there is profound fibrosis. In the early stages, altered internal milieu and physiology arising due to inflammation and release of cytokines might lead to deranged signaling pathways and islet dysfunction. Subsequently, development of fibrosis causes islet destruction. This suggests that endocrine deficiency in CP is multifactorial. Although the role of transcription factors (Pdx-1, MafA, NeuroD) on β-cell functions is understood, alterations in internal milieu of pancreatic tissue that affects β-cell functions in CP has not been elucidated. In this review, we summarize the factors that have an effect on islet functions. Understanding molecular events of β-cell dysfunction in CP can lead to the development of targeted preventive and therapeutic modalities.

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“…Finally, we assessed the effect of high glucose on isolated islets cultured in the presence of the saponin. It is well-known that high glucose induces oxidative stress on islets leading to widespread effects like protein carbonylation, 39 ROS formation, 40 lipid peroxidation, 36 DNA damage 41 and apoptosis. 42 In agreement to this, we found that islets exposed to high glucose showed increased formation of MDA and nitric oxide which, on the contrary, were reduced in islets treated with the saponin.…”

Section: Discussionmentioning

confidence: 99%

Triterpenoid Saponin from Momordica tuberosa (Cucurbitaceae) Stimulates Insulin Secretion from Isolated Mouse Pancreatic Islets and Provides Protection from Streptozotocin and High-Glucose Induced Injury

Samaddar¹,

Koneri²,

Varre³

et al. 2019

IJPER

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Background: Momordica tuberosa, belonging to the family Cucurbitaceae, is a tropical plant that is widely cultivated in the southern states of India. Objectives: Momordica tuberosa possesses a saponin, triterpenoid in nature that has been reported to exhibit anti-hyperglycaemic activity in vivo and in vitro. In this study we intend to isolate the saponin in pure purified state, assess its anti-hyperglycaemic activity on isolated mice pancreatic islets and evaluate its protective role against streptozotocin and highglucose induced islet injury, a central event in the pathogenesis of uncontrolled diabetes. Methods: Saponin purification and identification was carried out using chromatography followed by its structure elucidation IR, 1 H-NMR, 13 C-NMR and mass spectrometry. Islets were obtained from mice employing the pancreas perfusion method with collagenase type XI. Islet viability and specificity were determined by trypan blue-DTZ double staining followed by insulin secretion and intra-islet content measurement by ELISA. STZ-induced oxidative damage was assessed by lipid peroxidation and nitric oxide assay. High glucose-induced islet cell death/viability was assessed by MTT assay. Key findings: Approximately 650 islets per pancreas were recovered after isolation. The islets retained normal membrane integrity, viability and glucose-stimulated insulin secretion (GSIS). The saponin stimulated secretion of insulin from the isolated islets, protected from STZand high glucose-induced cytotoxicity and also from oxidative stress. Conclusion: The saponin acted as an insulin secretagogue and protected the islets from cytotoxic and oxidative stress induced by STZ and high glucose. We thus conclude that the saponin may be considered for effective use in IDDM.

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Control of hyperglycemia significantly improves oxidative stress profile of pancreatic islets

Cited by 9 publications

References 52 publications

Effect of Extracts Aqueous of Phragmites Australis on Carbohydrate Metabolism, Some Enzyme Activities and Pancreatic Islet Tissue in Alloxan-Induced Diabetic Rats

Effect of Extracts Aqueous of Phragmites Australis on Carbohydrate Metabolism, Some Enzyme Activities and Pancreatic Islet Tissue in Alloxan-Induced Diabetic Rats

β-Cell Dysfunction in Chronic Pancreatitis

Triterpenoid Saponin from Momordica tuberosa (Cucurbitaceae) Stimulates Insulin Secretion from Isolated Mouse Pancreatic Islets and Provides Protection from Streptozotocin and High-Glucose Induced Injury

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