Control of <i>Plum pox virus</i> through the use of genetically modified plants

Scorza, R.; Ravelonandro, M.

doi:10.1111/j.1365-2338.2006.01011.x

Cited by 8 publications

(4 citation statements)

References 54 publications

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“…HoneySweet) to PPV infection through aphid vectors and by graft inoculation. PPV was not naturally transmissible by aphids to plum, clone C5 trees in our experiment, which is in agreement with earlier suggestion (Scorza and Ravelonandro 2006). Minimal PPV symptoms observed in graft-inoculated trees disappeared during the years, and no PPV leaf symptoms were found after the nine years duration of field trial, and in the following three years.…”

Section: Discussionsupporting

confidence: 93%

Transgenic plum Prunus domestica L., clone C5 ( cv. HoneySweet) for protection against sharka disease

Polák

Kundu

Krška

et al. 2017

Journal of Integrative Agriculture

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Sharka caused by Plum pox virus (PPV) is the most harmful disease of stone fruits in Europe and elsewhere in the world. There is no highly PPV resistant cultivar of plum. Biotech approach has led to the development of resistance through genetic engineering. In this study, we evaluated a transgenic plum Prunus domestica L., clone C5 (cv. HoneySweet), where the PPV resistance is based on RNA interference (RNAi). Resistance in C5 plums has been evaluated for PPV, Prune dwarf virus (PDV), and Apple chlorotic leaf spot virus (ACLSV) in a regulated field trial in the Czech Republic for 12 years (2002-2013). Even under high and permanent infection pressure introduced through graft inoculation of the viruses, PPV has been detected in C5 trees only in several leaves situated close to the point of inoculum grafting in the first nine years. Mild symptoms of PPV disappeared year by year. No PPV symptoms were observed in the last three years and results of ELISA detection were negative. Similar results were obtained, when RT-PCR was used for PPV detection. No natural infection of PPV by aphids was recorded in C5 plums, even if PPV infected plants were growing in the same experimental field in close vicinity. Co-infections of PPV with PDV and/or ACLSV had practically no influence on stability of resistance in C5 trees. Twelve years of field testing in the Czech Republic clearly demonstrated the high level of resistance of C5 either to PPV infection by graft inoculation or natural infection through aphid vectors.

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Section: Discussionsupporting

confidence: 93%

Transgenic plum Prunus domestica L., clone C5 ( cv. HoneySweet) for protection against sharka disease

Polák

Kundu

Krška

et al. 2017

Journal of Integrative Agriculture

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“…Efforts towards the development of PPV‐resistant transgenics have also been particularly active, culminating with the validation through field trials of the resistance of the HoneySweet transgenic plum (Capote et al ., 2008; Hily et al ., 2004; Scorza and Ravelonandro, 2006) and its deregulation in the USA, making it one of the few virus‐resistant transgenic crops developed to marketability.…”

Section: Plum Pox Virus (Ppv)mentioning

confidence: 99%

Top 10 plant viruses in molecular plant pathology

Scholthof

Adkins²,

Czosnek

et al. 2011

Molecular Plant Pathology

1,032

606

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Many scientists, if not all, feel that their particular plant virus should appear in any list of the most important plant viruses. However, to our knowledge, no such list exists. The aim of this review was to survey all plant virologists with an association with Molecular Plant Pathology and ask them to nominate which plant viruses they would place in a 'Top 10' based on scientific/economic importance. The survey generated more than 250 votes from the international community, and allowed the generation of a Top 10 plant virus list for Molecular Plant Pathology. The Top 10 list includes, in rank order, (1) Tobacco mosaic virus, (2) Tomato spotted wilt virus, (3) Tomato yellow leaf curl virus, (4) Cucumber mosaic virus, (5) Potato virus Y, (6) Cauliflower mosaic virus, (7) African cassava mosaic virus, (8) Plum pox virus, (9) Brome mosaic virus and (10) Potato virus X, with honourable mentions for viruses just missing out on the Top 10, including Citrus tristeza virus, Barley yellow dwarf virus, Potato leafroll virus and Tomato bushy stunt virus. This review article presents a short review on each virus of the Top 10 list and its importance, with the intent of initiating discussion and debate amongst the plant virology community, as well as laying down a benchmark, as it will be interesting to see in future years how perceptions change and which viruses enter and leave the Top 10.

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“…The most successful instances of creating viral resistance in fruit crops, to date, are limited to papaya ringspot virus (PRSV) in papaya ( Carica papaya L.) and plum pox virus (PPV) in plum ( Prunus domestica L.) (Scorza and Ravelonandro, ; Souza et al ., ). In the former case, transformed genotypes have been introduced directly into commercial production following deregulation by APHIS in 1996, with acceptance into the Japanese market occurring as recently as 2011.…”

Section: Introductionmentioning

confidence: 99%

Engineering cherry rootstocks with resistance to Prunus necrotic ring spot virus through RNAi‐mediated silencing

Song

Sink

Walworth

et al. 2013

Plant Biotechnology Journal

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Summary Prunus necrotic ringspot virus (PNRSV) is a major pollen‐disseminated ilarvirus that adversely affects many Prunus species. In this study, an RNA interference (RNAi) vector pART27–PNRSV containing an inverted repeat (IR) region of PNRSV was transformed into two hybrid (triploid) cherry rootstocks, ‘Gisela 6’ (GI 148‐1) and ‘Gisela 7’(GI 148‐8)’, which are tolerant and sensitive, respectively, to PNRSV infection. One year after inoculation with PNRSV plus Prune Dwarf Virus, nontransgenic ‘Gisela 6’ exhibited no symptoms but a significant PNRSV titre, while the transgenic ‘Gisela 6’ had no symptoms and minimal PNRSV titre. The nontransgenic ‘Gisela 7’ trees died, while the transgenic ‘Gisela 7’ trees survived. These results demonstrate the RNAi strategy is useful for developing viral resistance in fruit rootstocks, and such transgenic rootstocks may have potential to enhance production of standard, nongenetically modified fruit varieties while avoiding concerns about transgene flow and exogenous protein production that are inherent for transformed fruiting genotypes.

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Control of Plum pox virus through the use of genetically modified plants

Cited by 8 publications

References 54 publications

Transgenic plum Prunus domestica L., clone C5 ( cv. HoneySweet) for protection against sharka disease

Transgenic plum Prunus domestica L., clone C5 ( cv. HoneySweet) for protection against sharka disease

Top 10 plant viruses in molecular plant pathology

Engineering cherry rootstocks with resistance to Prunus necrotic ring spot virus through RNAi‐mediated silencing

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