Control of induced thymidine kinase activity in the poxvirus-infected cell

McAuslan, B.R.

doi:10.1016/0042-6822(63)90152-1

Cited by 107 publications

(41 citation statements)

References 11 publications

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“…The main advantage of this technique, which uses fiberglass filters impregnated with poly(U), is the rapidity with which many samples can be screened for poly(A) content. In this report, we compare the properties of the rapid filter assay with those of the more time-consuming poly(U)-cellulose column assay (12 (17), and virus was purified by a modification (11) of the method of Joklik (18).…”

mentioning

confidence: 99%

Detection of Polyadenylic Acid Sequences in Viral and Eukaryotic RNA

Sheldon

Jurale

Kates

1972

Proc. Natl. Acad. Sci. U.S.A.

208

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A rapid and specific technique to detect polyriboadenylic acid sequences in RNA is described. The method depends upon the ability of RNAs that contain poly(A) sequences to associate specifically with poly(U) that has been immobilized on fiberglass filters by ultraviolet irradiation. A high proportion of the transcripts synthesized in vivo and in vitro from the vaccinia virus genome contain poly(A) sequences and bind to the poly(U) filters. Similarly, DNA-like RNA from the nucleus and from the cytoplasmic polyribosomes of HeLa cells is rich in species that bind to poly(U) filters. Poly(U) immobilized on cellulose powder is useful to make columns with a high capacity for the binding and purification of poly(A)-containing RNAs.

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mentioning

confidence: 99%

Detection of Polyadenylic Acid Sequences in Viral and Eukaryotic RNA

Sheldon

Jurale

Kates

1972

Proc. Natl. Acad. Sci. U.S.A.

208

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“…The data of Joklik and Merigan (34) indicate that virus messenger RNA does not combine with ribosomes to form polyribosomes in interferon-treated cells. This in turn means an inhibition of the initiation of virus protein synthesis.…”

Section: Discussionmentioning

confidence: 97%

“…Virus-directed protein synthesis is therefore important for the final uncoating of the genome (33); however, transcription of the parental genome can take place before complete uncoating of the virus. In fact, it has been shown that in the absence of protein synthesis, transcription of the parental genome takes place in an uncontrolled manner leading to increased production of viral messenger RNA (34).…”

Section: Dna Virusesmentioning

confidence: 99%

Studies on the Mechanism of Interferon Action

Friedman

1970

The Journal of General Physiology

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Interferon does not inactivate viruses or viral RNA. Virus growth is inhibited in interferon-treated cells, but apart from conferring resistance to virus growth, no other effect of interferon on cells has been definitely shown to take place. Interferon binds to cells even in the cold, but a period of incubation at 37 0 C is required for development of antiviral activity. Cytoplasmic uptake of interferon has not been unequivocally demonstrated. Studies with antimetabolites indicate that the antiviral action of interferon requires host RNA and protein synthesis. Experiments with 2-mercapto-l(f-4-pyridethyl) benzimidazole (MPB) suggest that an additional step is required between the binding and the synthesis of macromolecules. Interferon does not affect the adsorption, penetration, or uncoating of RNA or DNA viruses, but viral RNA synthesis is inhibited in cells infected with RNA viruses. The main action of interferon appears to be the inhibition of the translation of virus genetic information probably by inhibiting the initiation of virus protein synthesis.Interferon does not directly inactivate virus particles. It blocks an intracellular step involved in virus replication, probably virus-directed protein synthesis. Interferon-treated cells seem to develop this resistance to viruses through an active process involving cellular RNA and protein synthesis. The mechanism of action of interferon therefore involves the following two distinct problems: the changes which take place in an interferon-treated cell that lead to the development of resistance to virus growth, and how events in the virus growth cycle are modified by interferon treatment.The noteworthy properties of interferon should be accounted for by observations on its mechanism of action. These properties include inhibition of DNA and RNA virus replication, a remarkable selectivity of interferon action which results in inhibition of virus replication with as yet no detectable effect on cell metabolism, the variation in sensitivity of viruses to interferon, its species specificity, and its remarkable potency. What has been discovered so far about interferon action does provide data which help to explain some of these properties. At the end of this report I will try to show, at least provisionally, how current ideas on interferon action may explain some of its properties.

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“…Inhibition of DNA synthesis in vaccinia-infected cells has been shown to prevent switch-over from "early" to "]ate" viral protein synthesis resulting in the accumulation to abnormally high levels of early enzymes (8,10). Cells were therefore infected in the presence of 10 -3 M hydroxyurea and extracts examined for the presence of eytotoxin.…”

Section: Effect O / a N Inhibitor O/ D N A Synthesis On The Presence mentioning

confidence: 99%

Further studies on a vaccinia virus cytotoxin present in infected cell extracts: Identification as surface tubule monomer and possible mode of action

Burgoyne

Stephen

1979

Archives of Virology

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A vaccinia virus-specific cytotoxic protein (3--10 X 10(4) daltons) had previously been detected in extracts of infected HeLa cells. In this study the protein has been shown to be a late gene product and a virion component--almost certainly the monomer of the surface tubules of the vaccinia virion. The relationship of this cytotoxin to a number of early vaccinia-induced cytopathic effects was examined: it was not the mediator of vaccinia-induced early cell rounding, did not inhibit protein or RNA synthesis in cell-free systems or intact cells (after uptake-induction by hypertonic MgSO4), or cause the release of beta-glucuronidase from a crude preparation of HeLa cell lysosomes. Its possible role in vaccinia-induced cell degeneration, late in infection, is discussed.

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Control of induced thymidine kinase activity in the poxvirus-infected cell

Cited by 107 publications

References 11 publications

Detection of Polyadenylic Acid Sequences in Viral and Eukaryotic RNA

Detection of Polyadenylic Acid Sequences in Viral and Eukaryotic RNA

Studies on the Mechanism of Interferon Action

Further studies on a vaccinia virus cytotoxin present in infected cell extracts: Identification as surface tubule monomer and possible mode of action

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