Control of mastitis in dairy sheep and goats

Manning, Al; Vasileiou, N.G.C.; Crilly, JP

doi:10.12968/live.2021.26.3.161

Cited by 6 publications

(4 citation statements)

References 57 publications

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“…The aforementioned workers attributed their causes to the suckling lambs that play an important role in transmitting infection because they carry the pathogen in their nasopharynx (2,15). However, the outcome of the present work is in accordance with those authors Omaleki et al (15) and Van den et al (19), who isolated and identified other species of Mannheamia (M. haemolytica, M. ruminalis and M. glucosida) from multiple cases of acute mastitis.…”

Section: Discussionsupporting

confidence: 91%

“…Mastitis is regarded as an important productive disease contracting ranged-reared sheep with particular concern of those dairy and nursing ewes. Several studies have approved and confirmed the effective role of M. haemolytica as a primary and crucial etiology of mastitis in sheep which similar to those caused by Staphylococcus aureus, however, the infection percentages of the former microorganism are relatively higher (2,4,15). Consequently, the current work supports such type of mastitis in sheep of Mosul city.…”

Section: Discussionsupporting

confidence: 63%

“…Mastitis is known as inflammation of mammary glands including teats, teat canals and milk cistern of the udder. Its characterized by physical, chemical, and biological alteration of the udder and milk (1,2). Mastitis is regarded as a source of anxious for animal owners due to its adverse effect on animal hygiene and management as well as its influence on milk production, dairy manufacturing, wool and meat production.…”

Section: Introductionmentioning

confidence: 99%

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Isolation, identification and genetic analysis of Mannheimia haemolytica in ovine clinical mastitis in Mosul city

Rasheed¹,

Jwher²

2022

IJVS

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The aim of this study was to identify and diagnose of M. haemolytica strains as one of the most important causes of ovine clinical mastitis in Mosul city. One hundred and thirtythree milk samples were directly obtained from the udders of ewes infected by clinical mastitis from November 2020 to January 2021.Standard and conventional methods were followed for isolation and identification of M. haemolytica. Milk samples were cultured on blood agar 7% and MacConkey agar, then it was purified and was stained by Methylene blue. Later, different biochemical tests were conducted. Molecular identification of M. haemolytica depending on 16srRNA gene, followed by sequencing, similarity and phylogenetic tree was generated. The results showed that 62(46.61%) of samples were positive for bacterial isolation, biochemical tests and conventional PCR technique. Sequencing results showed that the positive samples were belonged to M. haemolytica strains. The similarity within strain Ib001 and within strain 39433 were 100%, and 99.47% respectively. Poor management was associated with the high level of mastitis caused by M. haemolytica, so the application of prophylactic programs should be followed to limit the spread of the disease.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

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Isolation, identification and genetic analysis of Mannheimia haemolytica in ovine clinical mastitis in Mosul city

Rasheed¹,

Jwher²

2022

IJVS

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“…Hassan and Yousif (6) found the 12.22% of subclinical mastitis in ewes in Al Anbar Province. Many articles showed that S. aureus is one of the most important bacteria causing mastitis in dairy animals including sheep (7,8). California mastitis test used as a quick field assay to determine subclinical mastitis of ewes and other animals, its Iraqi Journal of Veterinary Sciences, Vol.…”

Section: Introductionmentioning

confidence: 99%

Molecular and phylogenetic analysis of methicillin resistant Staphylococcus aureus isolated from subclinical mastitis in lactating ewes

Ahmed¹,

Yousif²

2021

IJVS

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This study was conducted for estimation of subclinical mastitis prevalence caused by Staphylococcus aureus (S. aureus) in apparent normal lactating ewes in the areas around Baghdad (Al-Rasheed, Al-Yousifya, Al-Latifya). 400 milk samples from 200 ewes were collected and examined by California mastitis test (CMT), Bacteriological examination was done by culturing on Blood agar, Mannitol salt agar, Staph-110 agar and Chrome agar then bacteria examined by Gram stain and biochemical test. Antibiotic sensitivity test and molecular assay by PCR were done to detect methicillin-resistant strains with the phylogenetic study. The results showed that 49/400 samples gave positive to CMT in the field and positive to S. aureus isolation. The percentage of subclinical mastitis in ewes was 12.25% in this study. All isolated bacteria were resistant to methicillin 100%. The result of PCR revealed that 16.32 % (8/49) of the MRSA were possess mecA gene, while no isolate carried the mecC gene, PCR-product for 8 positive samples of mecA target gene were sequenced, analyzed, and reported in Genbank-NCBI and obtained accession number and became a reference to Iraq and the Middle East and the world. Seven samples that match the global results by 100%, and one sample contains a mutation at the site 1539, Nucleotide. Our conclusion revealed that detection of subclinical mastitis in sheep very important caused by S. aureus and all S. aureus was resistant to methicillin and some strains possess mecA gene. However, absence of mec A in other MRSA isolates requires detecting the alternative genetic possibilities related to the resistance profile and fragment of gene used.

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