Control of methicillin-resistant Staphylococcus aureus in planktonic form and biofilms: A biocidal efficacy study of nonthermal dielectric-barrier discharge plasma

Joshi, Suresh G.; Paff, Michelle; Friedman, Gary D.; Fridman, Greg; Fridman, Alexander; Brooks, Ari D.

doi:10.1016/j.ajic.2009.11.002

Cited by 159 publications

(153 citation statements)

References 18 publications

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“…These metabolites can inhibit proinflammatory gene expression to enhance survival of some pathogens (50) and are also detrimental to certain pathogens, such as Staphylococcus aureus, that are sensitive to polycations (51). Interestingly, strains of S. aureus frequently associated with skin infections have a high association of carriage of the arginine catabolic mobile element (ACME), which, in addition to bearing genes necessary for arginine catabolism, also bears a polyamine resistance gene, speG, which has recently been shown to aid in a mouse model of staphylococcal skin infections (52).…”

Section: Figmentioning

confidence: 99%

Streptococcus pyogenes Arginine and Citrulline Catabolism Promotes Infection and Modulates Innate Immunity

2014

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A bacterium's ability to acquire nutrients from its host during infection is an essential component of pathogenesis. For the Gram-positive pathogen Streptococcus pyogenes, catabolism of the amino acid arginine via the arginine deiminase (ADI) pathway supplements energy production and provides protection against acid stress in vitro. Its expression is enhanced in murine models of infection, suggesting an important role in vivo. To gain insight into the function of the ADI pathway in pathogenesis, the virulence of mutants defective in each of its enzymes was examined. Mutants unable to use arginine (⌬ArcA) or citrulline (⌬ArcB) were attenuated for carriage in a murine model of asymptomatic mucosal colonization. However, in a murine model of inflammatory infection of cutaneous tissue, the ⌬ArcA mutant was attenuated but the ⌬ArcB mutant was hyperattenuated, revealing an unexpected tissue-specific role for citrulline metabolism in pathogenesis. When mice defective for the arginine-dependent production of nitric oxide (iNOS ؊/؊ ) were infected with the ⌬ArcA mutant, cutaneous virulence was rescued, demonstrating that the ability of S. pyogenes to utilize arginine was dispensable in the absence of nitric oxide-mediated innate immunity. This work demonstrates the importance of arginine and citrulline catabolism and suggests a novel mechanism of virulence by which S. pyogenes uses its metabolism to modulate innate immunity through depletion of an essential host nutrient.

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Section: Figmentioning

confidence: 99%

Streptococcus pyogenes Arginine and Citrulline Catabolism Promotes Infection and Modulates Innate Immunity

2014

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“…CAP is receiving increasing attention in medical applications due to its high bactericidal activity and low toxicity towards healthy human cells [5]. CAP generates reactive oxygen and nitrogen species (RONS), and the oxidative stress caused by RONS is the killing mechanism that damages proteins and nucleic acids [6,7]. The CAP sources typically used for medical treatments are atmospheric pressure plasma jets pens.…”

mentioning

confidence: 99%

Combination of Cold Atmospheric Plasma and Vitamin C Effectively Disrupts Bacterial Biofilms

Pandit¹,

Mokkapati²,

Helgadottir³

et al. 2017

Clin Microbiol

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Cold atmospheric plasma (CAP) is increasingly used in medical applications for eradication of bacterial and tumor cells. CAP treatment devices, known as plasma jet pens, produce reactive oxygen and nitrogen species at atmospheric pressure and room temperature. The produced reactive species are concentrated in a small and precisely defined area, allowing for high precision medical treatments. CAP has been demonstrated as very effective against planktonic bacterial cells. Unfortunately, bacterial cells in biofilms are typically aggregated and protected by dense exopolymeric matrix, synthesized and secreted by the bacterial community. The main limitation in using CAP against bacterial biofilms is the thick protective matrix of extracellular polymers that shields bacterial cells within this complex architecture. CAP has also been shown to effectively eradicate tumor cells, but the main current limitation is the susceptibility of the surrounding healthy tissues to higher doses. We have recently demonstrated that vitamin C, a natural food supplement, can be used to destabilize bacterial biofilms and render them more susceptible to the CAP killing treatment. Here we discuss the possible impact that a pre-treatment with vitamin C could have on CAP applications in medicine. Specifically, we argue that vitamin C could enhance the effectiveness of CAP treatments against both the bacterial biofilms and some selected tumors.

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“…Another new approach in combating organisms growing in biofilms is cold atmospheric plasma, which has demonstrated its bactericidal, virucidal, and fungicidal properties due to the generation of reactive species, charged molecules, and photons (20,29,34). Numerous studies have demonstrated the effectiveness of gas discharge or cold atmospheric plasma in killing only planktonic microorganisms (14,17,18,29). The killing efficacies were dependent on the plasma exposure time (10 s to 10 min), the material the microorganisms were located on, and the cell density.…”

mentioning

confidence: 99%

Contact-Free Inactivation of Candida albicans Biofilms by Cold Atmospheric Air Plasma

Maisch

Shimizu

Isbary

et al. 2012

Appl Environ Microbiol

100

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ABSTRACTCandida albicansis one of the main species able to form a biofilm on almost any surface, causing both skin and superficial mucosal infections. The worldwide increase in antifungal resistance has led to a decrease in the efficacy of standard therapies, prolonging treatment time and increasing health care costs. Therefore, the aim of this work was to demonstrate the applicability of atmospheric plasma at room temperature for inactivatingC. albicansgrowing in biofilms without thermally damaging heat-sensitive materials. This so-called cold atmospheric plasma is produced by applying high voltage to accelerate electrons, which ionize the surrounding air, leading to the production of charged particles, reactive species, and photons. A newly developed plasma device was used, which exhibits a large plasma-generating surface area of 9 by 13 cm (117 cm2). Different time points were selected to achieve an optimum inactivation efficacy range of ≥3 log10to 5 log10reduction in CFU per milliliter, and the results were compared with those of 70% ethanol. The results obtained show that contact-free antifungal inactivation ofCandidabiofilms by cold atmospheric plasma is a promising tool for disinfection of surfaces (and items) in both health care settings and the food industry, where ethanol disinfection should be avoided.

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Control of methicillin-resistant Staphylococcus aureus in planktonic form and biofilms: A biocidal efficacy study of nonthermal dielectric-barrier discharge plasma

Cited by 159 publications

References 18 publications

Streptococcus pyogenes Arginine and Citrulline Catabolism Promotes Infection and Modulates Innate Immunity

Streptococcus pyogenes Arginine and Citrulline Catabolism Promotes Infection and Modulates Innate Immunity

Combination of Cold Atmospheric Plasma and Vitamin C Effectively Disrupts Bacterial Biofilms

Contact-Free Inactivation of Candida albicans Biofilms by Cold Atmospheric Air Plasma

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