Control of mycobacteriosis in zebrafish ( Danio rerio ) mucosally vaccinated with heat-inactivated Mycobacterium bovis

Risalde, María Á.; López, Vladimir; Contreras, Marinela; Mateos-Hernández, Lourdes; Gortázar, Christian; Fuente, José de la

doi:10.1016/j.vaccine.2018.06.042

Cited by 29 publications

(49 citation statements)

References 48 publications

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“…using the zebrafish model. The zebrafish model of tuberculosis has been used in our laboratory for the characterization of the protective response elicited after vaccination with heat inactivated M. bovis (IV) [27,28]. In these experiments, we showed a reduction of mycobacteriosis in vaccinated fish and suggested that the innate immune response mediated by the C3 pathway activated through TLR-AKR2-IL-1β and other proinflammatory cytokines acted as the protective mechanism against infection.…”

Section: Discussionmentioning

confidence: 85%

“…Zebrafish were sectioned sagittally, and half of them were immediately fixed in 10% neutral buffered formalin for 24 h at 21 • C, dehydrated in a graded series of ethanol, immersed in xylol, and embedded in paraffin wax using an automatic processor. Sections were cut at 4 µm and stained with haematoxylin and eosin (HE) and Ziehl-Neelsen (ZN) staining following standard procedures [27,28]. The small size of the zebrafish and the sagittal sections to divide them in two portions for molecular and histopathological studies permitted a histological assessment only in central nervous system, branchial arches, muscle, skin, liver, intestine, and gonads.…”

Section: Histopathologymentioning

confidence: 99%

“…To address the possibility of using vaccination with α-Gal for the control of tuberculosis, in this study, we used the zebrafish Danio rerio (Hamilton, 1822) animal model. Zebrafish is a model organism for the study of immune mechanisms and new effective vaccines and control strategies against tuberculosis [24][25][26][27][28]. Additionally, zebrafish do not produce α-Gal and were recently shown to reproduce some features of the human immune response to this molecule as a model for the study of the AGS [29].…”

Section: Introductionmentioning

confidence: 99%

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Vaccination with Alpha-Gal Protects Against Mycobacterial Infection in the Zebrafish Model of Tuberculosis

et al. 2020

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The alpha-Gal syndrome (AGS) is associated with tick bites that can induce in humans high levels of IgE antibodies against the carbohydrate Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) present in glycoproteins and glycolipids from tick saliva that mediate primarily delayed anaphylaxis to mammalian meat consumption. It has been proposed that humans evolved by losing the capacity to synthesize α-Gal to increase the protective immune response against pathogens with this modification on their surface. This evolutionary adaptation suggested the possibility of developing vaccines and other interventions to induce the anti-α-Gal IgM/IgG protective response against pathogen infection and multiplication. However, the protective effect of the anti-α-Gal immune response for the control of tuberculosis caused by Mycobacterium spp. has not been explored. To address the possibility of using vaccination with α-Gal for the control of tuberculosis, in this study, we used the zebrafish-Mycobacterium marinum model. The results showed that vaccination with α-Gal protected against mycobacteriosis in the zebrafish model of tuberculosis and provided evidence on the protective mechanisms in response to vaccination with α-Gal. These mechanisms included B-cell maturation, antibody-mediated opsonization of mycobacteria, Fc-receptor (FcR)-mediated phagocytosis, macrophage response, interference with the α-Gal antagonistic effect of the toll-like receptor 2 (TLR2)/nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB)-mediated immune response, and upregulation of pro-inflammatory cytokines. These results provided additional evidence supporting the role of the α-Gal-induced immune response in the control of infections caused by pathogens with this modification on their surface and the possibility of using this approach for the control of multiple infectious diseases.

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Section: Discussionmentioning

confidence: 85%

Section: Histopathologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Vaccination with Alpha-Gal Protects Against Mycobacterial Infection in the Zebrafish Model of Tuberculosis

et al. 2020

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“…It was also a promising new model for tuberculosis vaccine research, including the pre-clinical identification of vaccine antigens [16,17,36,41,61,67];). Other species of Mycobacterium have also been studied, such as M. bovis [52,73] and M. abscessos [7]. M. bovis is most common in cattle, but also affects humans.…”

Section: Animals and Human Vaccinesmentioning

confidence: 99%

Zebrafish as an alternative animal model in human and animal vaccination research

et al. 2020

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Much of medical research relies on animal models to deepen knowledge of the causes of animal and human diseases, as well as to enable the development of innovative therapies. Despite rodents being the most widely used research model worldwide, in recent decades, the use of the zebrafish (Danio rerio) model has exponentially been adopted among the scientific community. This is because such a small tropical freshwater teleost fish has crucial genetic, anatomical and physiological homology with mammals. Therefore, zebrafish constitutes an excellent experimental model for behavioral, genetic and toxicological studies which unravels the mechanism of various human diseases. Furthermore, it serves well to test new therapeutic agents, such as the safety of new vaccines. The aim of this review was to provide a systematic literature review on the most recent studies carried out on the topic. It presents numerous advantages of this type of animal model in tests of efficacy and safety of both animal and human vaccines, thus highlighting gains in time and cost reduction of research and analyzes.

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“…Histological examination of the adult zebrafish- M . marinum infection system has been instrumental in advancing our understanding of mycobacterial virulence, granuloma macrophage epithelioid differentiation, vascularization, and the adaptive immune response to mycobacterial infection (Cronan et al, 2016; Oehlers et al, 2015; Parikka et al, 2012; Prouty et al, 2003; Risalde et al, 2018; Swaim et al, 2006; van der Sar et al, 2004; Volkman et al, 2004; Weerdenburg et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

High content analysis of granuloma histology and neutrophilic inflammation in adult zebrafish infected with Mycobacterium marinum

Cheng

Kam

Johansen

et al. 2019

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Infection of zebrafish with natural pathogen Mycobacterium marinum is a useful surrogate for studying the human granulomatous inflammatory response to infection by Mycobacterium tuberculosis. The adaptive immune system of the adult stage zebrafish offers an advance on the commonly used embryo infection model as adult zebrafish form granulomas with striking similarities to human-M. tuberculosis granulomas. Here, we present workflows to perform high content analyses of granulomas in adult zebrafish infected with M. marinum by cryosectioning to take advantage of strong endogenous transgenic fluorescence adapted from common zebrafish embryo infection tools. Specific guides to classifying granuloma necrosis and organisation, quantifying bacterial burden and leukocyte infiltration of granulomas, and visualizing extracellular matrix remodelling and foam cell formation are also provided. We use these methods to characterize neutrophil recruitment to M. marinum granulomas across time and find an inverse relation to granuloma necrosis suggesting granuloma necrosis is not a marker of immunopathology in the natural infection system of the adult zebrafish-M. marinum pairing. The methods can be easily translated to studying the zebrafish adaptive immune response to other chronic and granuloma-forming pathogens.

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Control of mycobacteriosis in zebrafish ( Danio rerio ) mucosally vaccinated with heat-inactivated Mycobacterium bovis

Cited by 29 publications

References 48 publications

Vaccination with Alpha-Gal Protects Against Mycobacterial Infection in the Zebrafish Model of Tuberculosis

Vaccination with Alpha-Gal Protects Against Mycobacterial Infection in the Zebrafish Model of Tuberculosis

Zebrafish as an alternative animal model in human and animal vaccination research

High content analysis of granuloma histology and neutrophilic inflammation in adult zebrafish infected with Mycobacterium marinum

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