Control of Oocyte Growth and Development by Intercellular Communication Within the Follicular Niche

El‐Hayek, Stephany; Clarke, Hugh J.

doi:10.1007/978-3-319-31973-5_8

Cited by 62 publications

(40 citation statements)

References 305 publications

(285 reference statements)

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“…Notably, Metagenomic analysis was used to find that the most abundant genera in Nellore Cattle (beef cattle) is Aeribacillus, Bacteroides, Clostridium, Ruminococcus, Rikenella, Alistipes, Bacillus, Eubacterium and Prevotella spp. which comprised of 30-40% of total bacteria in the vaginal microflora (El-Hayek et al 2016). In agreement with previous studies, streptococcus spp., E. coliand lactobacillus spp.were also found in the vagina of beef cattle.…”

Section: Analysis Of Vaginal Microbial Community By Real-time Pcrsupporting

confidence: 92%

Comparison of vaginal microbial community structure of beef cattle between luteal phase and follicular phase

Wang

Liu

Nesengani

et al. 2018

IJAR

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The aim of the current study was to evaluate the vaginal microbial community structure in beef cattle between follicular and luteal phase. Fifteen healthy beef cattle were subjected to an estrus synchronization protocol, and vaginal samples were collected in the follicular and luteal phases. Polymerase chain reaction (PCR) followed by denaturing gradient gel electrophoresis (PCR-DGGE) and real-time PCR were used to determine the vaginal community structure. The results showed that the vaginal microflora of beef cattle mainly comprised of Aerococcus vaginalis, Aerococcus viridans, Haemophilus somnus, Streptococcus pluranimalium, Psychrobacter marincola, Escherichia coli, and Sphingomonas roseiflava. There was no significant difference in the total number of bacteria in the vaginas of beef cattle between the follicular and luteal phase. The quantity of vaginal Streptococcus spp. in the luteal phase was significantly higher than in the follicular phase, while the number of vaginal Lactobacillus spp. in the follicular phase was significantly higher than in the luteal phase. The results indicated that there was moderate significant difference in the structure of the vaginal microbial community in beef cattle between the luteal phase and follicular phase.

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Section: Analysis Of Vaginal Microbial Community By Real-time Pcrsupporting

confidence: 92%

Comparison of vaginal microbial community structure of beef cattle between luteal phase and follicular phase

Wang

Liu

Nesengani

et al. 2018

IJAR

View full text Add to dashboard Cite

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“…On the other hand, close interactions between the oocyte and the surrounding somatic cells are required for the follicle formation; proliferation and differentiation of granulosa cells; and steroid hormone production. Thus, an impaired oocyte-granulosa cell complex would lead to defective folliculogenesis and steroidogenesis [ 47 – 50 ]. Also, the ovarian ECM has various impacts on the oocyte maturation and granulose cell secretion [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Decellularized human ovarian scaffold based on a sodium lauryl ester sulfate (SLES)-treated protocol, as a natural three-dimensional scaffold for construction of bioengineered ovaries

et al. 2018

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BackgroundThe increasing number of patients with ovarian insufficiency due to autoimmune disorders, genetic predisposition, or iatrogenic effects of treatment such as cancer therapies necessitates an urgent measure to find a safe and transplantable alternative ovary. A bioengineered ovary is one of the strategies on which the researchers have recently been working. An engineered ovary should be able to mimic the natural ovary aspects. Recent studies suggest that the decellularized organ-specific extracellular matrix-based scaffolds can serve as a native niche to bioengineering artificial organs. Therefore, we established a human decellularized ovarian scaffold based on a sodium lauryl ester sulfate (SLES)-treated process, as an optimized protocol.MethodsThe human ovary samples were decellularized with 1% SLES for 48 h followed by DNase I in PBS for 24 h, and then thoroughly rinsed in PBS to remove the cell remnants and chemical reagents. Efficient cell removal was confirmed by DNA content analysis, hematoxylin and eosin, and Hoechst staining. Preservation assessment of the extracellular matrix structures was performed by immunohistochemistry, histological staining, and scanning electron microscopy. An MTT test was done to assess the in vitro scaffold’s cytocompatibility, and finally in vivo studies were performed to evaluate the biocompatibility, bioactivity, and secretion functions of the ovarian grafts made of primary ovarian cells (POCs) on the decellularized scaffolds.ResultsEvidence provided by SEM, histochemical, and immunohistochemical analyses showed that the ovarian extracellular matrix was preserved after decellularization. Moreover, MTT test indicated the suitable cytocompatibility of the scaffolds. The in vivo assessment showed that the POCs kept their viability and bioactivity, and reconstructed the primordial or primary follicle-like structures within the scaffolds after transplantation. Immunostaining characterized somatic cells that were capable of expressing steroid hormone receptors; also, as a marker of granulosa cell, inhibin-α immunostaining demonstrated these cells within the grafts. Additionally, hormone assessment showed that serum estradiol and progesterone levels were significantly higher in ovariectomized rats with ovarian cells-seeded grafts than those with or without decellularized scaffold grafts.ConclusionsA human ovary-specific scaffold based on a SLES-decellularized protocol as a biomimicry of the natural ovarian niche can be an ideal scaffold used to reconstruct the ovary.Electronic supplementary materialThe online version of this article (10.1186/s13287-018-0971-5) contains supplementary material, which is available to authorized users.

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“…PCOS is positively correlated with insulin resistance. Testosterone levels were markedly higher in the PCOS group rats than in vehicle control group rats, presumably because insulin resistance might block the conversion of androgen substrates to estradiol 10 . Androgens are synthesized in theca cells and then transported to the granulosa cells where P450 aromatase converts the androgens to estrone and estradiol 11 .…”

Section: Fig 4: Magnification -10x Section Of Ovary From Pcos Groupmentioning

confidence: 87%

Untitled

2018

IJPSR

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Introduction: There are numerous animal models available for polycystic ovary syndrome (PCOS), a fully convincing model for PCOS reflecting all pathologic condition of PCOS seen in humans is not available due to difficulties in inducing all pathologies in rat models. If so, it will help to test drug to treat PCOS. The main goal of this study is to create a new model to reflect all pathologic conditions of PCOS seen in humans using non-steroidal aromatase inhibitor letrozole and fructose in rats. Methods: Twelve rats were divided into two groups containing 6 rats each. Vehicle control group rats received 1% aqueous solution of carboxmethlycellulose (CMC) once daily orally for 28 days and PCOS group was administered letrozole at a concentration of 1 mg/kg dissolved in 1% CMC p.o. once daily for 28 days. Along with these rats were allowed free access of 10% fructose solution daily. During experimental period, vaginal smears were collected daily for estrus cycle determination. Rats were sacrificed and blood sample were collected for hormonal and other biochemical assays. Ovaries were removed to proceed with histolopathological study. Results: When compared to vehicle control group, ovaries from PCOS group showed high incidence of ovarian cyst with incomplete luteinization and decreased number of corpus lutea. Although serum estradiol and progesterone levels were reduced, testosterone levels were elevated, as were levels of luteinizing hormone (LH). Serum follicle-stimulating hormone (FSH) levels were markedly increased in the PCOS group. The PCOS group rats showed significant increase in insulin resistance with compensatory significant hyperinsulinemia than the vehicle control group. Conclusions: Our study was completely fulfilling and convincing as in human PCOS, this rat model is fully convincing for studying PCOS and in several ways, it is similar to the human PCOS.

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Control of Oocyte Growth and Development by Intercellular Communication Within the Follicular Niche

Cited by 62 publications

References 305 publications

Comparison of vaginal microbial community structure of beef cattle between luteal phase and follicular phase

Comparison of vaginal microbial community structure of beef cattle between luteal phase and follicular phase

Decellularized human ovarian scaffold based on a sodium lauryl ester sulfate (SLES)-treated protocol, as a natural three-dimensional scaffold for construction of bioengineered ovaries

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