Control of Orange Rust and Brown Rust of Sugarcane with Systemic Fungicides

Koch, Gabriel; Ruaro, Lucimeris; Calegario, Renata Faier; Filho, João Carlos Bespalhok; Daros, Edelclaiton; Oliveira, Ricardo Augusto de; Duarte, Henrique da Silva Silveira

doi:10.1007/s12355-020-00939-3

Cited by 2 publications

(2 citation statements)

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“…In addition, the process of replacing susceptible cultivars is slow, owing to the following reasons: firstly, sugarcane is a perennial crop, replanting in China approximately every two to three years; secondly, only a few new varieties were generally released for commercial production each year; thirdly, sugarcane, being a vegetatively propagated crop, requires a longer period to produce adequate planting material for extensive cultivated areas [12]. Therefore, chemical control of SCBR emerges as the obvious choice without a rapid replacement of susceptible cultivars with resistant ones [13].…”

Section: Introductionmentioning

confidence: 99%

“…Early monitoring is crucial for P. melanocephala epidemic forecasting and making informed decisions on fungicide application to effectively control SCBR, particularly when the disease symptoms are masked, or the suspected symptoms appear in the sugarcane leaves [12,13]. However, the accurate identification of SCBR at the early stage of the disease development is hampered by several difficulties.…”

Section: Introductionmentioning

confidence: 99%

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Development and Evaluation of a Loop-Mediated Isothermal Amplifcation (LAMP) Assay for Specific and Sensitive Detection of Puccinia melanocephala Causing Brown Rust in Sugarcane

Wu,

Wang,

Wang

et al. 2024

Agronomy

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Sugarcane brown rust (SCBR), caused by Puccinia melanocephala, is a destructive fungal disease that has extensively spread in the sugarcane-cultivating regions across the world. Early monitoring plays an important role in predicting the P. melanocephala epidemic and managing SCBR. However, accurately identifying SCBR based on symptoms and urediniospore morphology at the initial stage is a challenge. Further, it is tedious, time-consuming, labor-intensive, and requires expensive equipment to detect P. melanocephala using PCR-based methods. Loop-mediated isothermal amplification (LAMP) technology is renowned for its speed, simplicity, and low equipment requirements for specifically and sensitively identifying many pathogens. Therefore, in this study, a novel and highly sensitive LAMP assay was developed for the specific detection of P. melanocephala in sugarcane. Here, the internal transcribed spacer (ITS) sequence of P. melanocephala was selected as the target gene for LAMP primer design. Based on the color change of SYBR Green I and gel electrophoresis, specific LAMP primers were screened. Further, the optimal reaction conditions for the LAMP assay were determined at 63 °C for 60 min. The LAMP assay showed a high degree of specificity for the detection of P. melanocephala in sugarcane, with no cross-reactivity with other fungal pathogens. The established LAMP protocol was highly sensitive and can be used to detect as low as 1 pg/μL of P. melanocephala plasmid DNA, which is comparable to that of nested PCR and ~100 times more sensitive than conventional PCR. Finally, the detection rate of the LAMP method was higher than that of conventional and nested PCR in field samples.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%