Control of osteoblast arrangement by osteocyte mechanoresponse through prostaglandin E2 signaling under oscillatory fluid flow stimuli

Matsuzaka, Tadaaki; Matsugaki, Aira; Nakano, Takayoshi

doi:10.1016/j.biomaterials.2021.121203

Cited by 26 publications

(14 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in actual life, both static and dynamic loads are applied to the bone, and the adaptive responses to dynamic loads play important roles in biological function [43,44]. In particular, our recent findings suggest that the dynamic loading condition realizes the anisotropic bone matrix microstructure by controlling the osteoblast arrangement [45]. An expanded ex vivo bone culture model that also enables the application and regulation of dynamic loads is required to further clarify the mechanism of collagen/apatite organization associated with mechanical loading.…”

Section: Discussionmentioning

confidence: 96%

A Novel Ex Vivo Bone Culture Model for Regulation of Collagen/Apatite Preferential Orientation by Mechanical Loading

Watanabe

Matsugaki

Ishimoto

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

The anisotropic microstructure of bone, composed of collagen fibers and biological apatite crystallites, is an important determinant of its mechanical properties. Recent studies have revealed that the preferential orientation of collagen/apatite composites is closely related to the direction and magnitude of in vivo principal stress. However, the mechanism of alteration in the collagen/apatite microstructure to adapt to the mechanical environment remains unclear. In this study, we established a novel ex vivo bone culture system using embryonic mouse femurs, which enabled artificial control of the mechanical environment. The mineralized femur length significantly increased following cultivation; uniaxial mechanical loading promoted chondrocyte hypertrophy in the growth plates of embryonic mouse femurs. Compressive mechanical loading using the ex vivo bone culture system induced a higher anisotropic microstructure than that observed in the unloaded femur. Osteocytes in the anisotropic bone microstructure were elongated and aligned along the long axis of the femur, which corresponded to the principal loading direction. The ex vivo uniaxial mechanical loading successfully induced the formation of an oriented collagen/apatite microstructure via osteocyte mechano-sensation in a manner quite similar to the in vivo environment.

show abstract

Section: Discussionmentioning

confidence: 96%

A Novel Ex Vivo Bone Culture Model for Regulation of Collagen/Apatite Preferential Orientation by Mechanical Loading

Watanabe

Matsugaki

Ishimoto

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, prostaglandin E2 signaling by osteocytes was identified as a potential inducer of osteoblast alignment (Matsuzaka et al, 2021). In this study, osteocytes might have been differentiated from hBMSCs but most likely only under influence of mechanical stimulation and towards the end of the culture period (Akiva et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Characterization of three-dimensional bone-like tissue growth and organization under influence of curvature and directional fluid flow

Wildt

Zhao

Lauwers

et al. 2022

Preprint

View full text Add to dashboard Cite

The transition in the field of bone tissue engineering from bone regeneration to three-dimensional in vitro models has come with the challenge of recreating a dense and anisotropic bone-like extracellular matrix with cell culture. The creation of such an organized bone-like extracellular matrix has received little attention thus far. Although the mechanism by which bone extracellular matrix gains its structure is not fully understood, curvature (especially concavities), mechanical loading due to deformations or directional fluid flow, and osteocyte signaling have been identified as potential contributors. Here, guided by computational simulations, we evaluated three-dimensional cell and bone-like tissue growth and organization in a concave channel with and without directional fluid flow stimulation. Human bone-marrow derived mesenchymal stromal cells were seeded on donut-shaped silk fibroin scaffolds and stimulated to undergo osteogenic differentiation for 42 days statically or in a flow perfusion bioreactor. Constructs were investigated for cell distribution, and tissue growth and organization on day 14, 28, and 42. As a result, directional fluid flow was able to improve bone-like tissue growth but not organization. After 28 days of culture, when osteogenic differentiation was likely accomplished, cells tended to have a small preference for orientation in the tangential (i.e., circumferential) direction of the channel. Based on our results, we suggest that three-dimensional bone-like tissue anisotropy might be guided by curvature, while extracellular matrix production can be increased through the application of fluid shear stress. With this study, an initial attempt in three-dimensions was made to improve the resemblance of in vitro produced bone-like extracellular matrix to the physiological bone extracellular matrix.

show abstract

“…It is also possible to control bone matrix orientation genetically. 46) The presence of genes, cell adhesion mechanisms, and cell-cell communication substances that cause such orientation are among the most important factors in developing next-generation medical devices with hard tissue compatibility (Fig. 4).…”

Section: Induction Of Bone Orientation By Metal Additive Manufacturingmentioning

confidence: 99%

Review—Metal Additive Manufacturing of Titanium Alloys for Control of Hard Tissue Compatibility

2023

Self Cite

View full text Add to dashboard Cite

Metal additive manufacturing is a powerful tool for providing the desired functional performance of hard tissue biomaterials through a three-dimensional structural design. It is essential to use the interactions between living organisms and materials for functional hard tissue reconstruction. In particular, anisotropic high-performance materials that imitate bone tissue properties are required for regaining bone functionality based on collagen/apatite microstructure. This review article describes the current development of controlling hard tissue compatibility by additive manufacturing of titanium alloys, including our recent findings on the bone medical devices for guiding the anisotropic bone microstructure.

show abstract

Control of osteoblast arrangement by osteocyte mechanoresponse through prostaglandin E2 signaling under oscillatory fluid flow stimuli

Cited by 26 publications

References 63 publications

A Novel Ex Vivo Bone Culture Model for Regulation of Collagen/Apatite Preferential Orientation by Mechanical Loading

A Novel Ex Vivo Bone Culture Model for Regulation of Collagen/Apatite Preferential Orientation by Mechanical Loading

Characterization of three-dimensional bone-like tissue growth and organization under influence of curvature and directional fluid flow

Review—Metal Additive Manufacturing of Titanium Alloys for Control of Hard Tissue Compatibility

Contact Info

Product

Resources

About