Control of osteogenesis by the canonical Wnt and BMP pathways in vivo

Marcellini, Sylvain; Henríquez, Juan Pablo; Bertin, Ariana

doi:10.1002/bies.201200061

Cited by 68 publications

(45 citation statements)

References 109 publications

(129 reference statements)

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“…Osteogenesis depends on an ordered sequence of gene activation, starting with bmp2 pathway which usually triggers mRNA runx2 transcription, one of the first factors directing the differentiation of precursor stem cells towards an osteoblastic lineage [51]. We found a sequential activation of both genes in comparison with the 28 years old subjects, whilst basically no significant changes were detected in comparison with 16 years cells.…”

Section: Mathematical Analysis and Modelingmentioning

confidence: 85%

Human Periosteal Derived Stem Cell Potential: The Impact of age

Ferretti

Lucarini

Andreoni

et al. 2014

Stem Cell Rev and Rep

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There is a great deal of interest in the understanding of possible age-related changes in Mesenchymal Stem Cells in view of their use for regenerative medicine applications. Given to the outmost standing of periosteum in bone biology and to probe data for a cell-based therapy promoting graft osseointegration in the elderly, we tried to identify specific aging markers or pattern of expression in human periosteal precursor cells. Immunohistochemical detection of Ki67 and p53, Nitric Oxide production and qRT- PCR of a selected gene panel for osteoblastic differentiation, bone remodeling and stemness were evaluated. We confirmed that both Ki67 and p53 are noteworthy indicators of senescence in human periosteal precursor cells and their expression significantly correlate with cell NO production. Moreover, cell age affects genes involved in bone remodeling, with a significant increase in interleukin-6 mRNA expression and receptor activator of nuclear factor kappa-B ligand/osteoprotegerin ratio. The analysis of mRNAs of genes involved in pluripotency regulation and self-renewal of stem cells, evidenced changes at least in part related to bone remodeling. We believe that this is the first study taking on age-related changes in human periosteal precursor cells, and paving the way toward new regenerative medicine strategies in bone aging and/or bone metabolic diseases.

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Section: Mathematical Analysis and Modelingmentioning

confidence: 85%

Human Periosteal Derived Stem Cell Potential: The Impact of age

Ferretti

Lucarini

Andreoni

et al. 2014

Stem Cell Rev and Rep

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“…The key signaling pathways, via which GFs guide osteogenic cell differentiation, are the TGF-β/BMP- and Wnt-mediated pathways [19,47]. While the BMP pathway ensures differentiation of MSCs into osteo-chondro-progenitors (OCPs), the Wnt pathway is essential for subsequent osteoblastic commitment, i.e., Wnt acts ‘downstream’ of BMP to ensure that OCPs differentiate into osteoblasts and not chondroblasts [47].…”

Section: Resultsmentioning

confidence: 99%

“…While the BMP pathway ensures differentiation of MSCs into osteo-chondro-progenitors (OCPs), the Wnt pathway is essential for subsequent osteoblastic commitment, i.e., Wnt acts ‘downstream’ of BMP to ensure that OCPs differentiate into osteoblasts and not chondroblasts [47]. Genes associated with both TGF-β/BMP and Wnt pathway (Wnt receptors) were upregulated at day 7 [34,35] and day 14 [34] on SLA and SLActive surfaces, suggesting the occurrence of osteogenic differentiation at these time points.…”

Section: Resultsmentioning

confidence: 99%

Genomic analyses of early peri-implant bone healing in humans: a systematic review

Shanbhag

Shanbhag²,

Stavropoulos

2015

Int J Implant Dent

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ObjectiveThe objective of the study was to systematically review the literature for studies reporting gene expression analyses (GEA) of the biological processes involved in early human peri-implant bone healing.MethodsElectronic databases (MEDLINE, EMBASE) were searched in duplicate. Controlled and uncontrolled studies reporting GEA of human peri-implant tissues - including ≥5 patients and ≥2 time points - during the first 4 weeks of healing were eligible for inclusion. Methodological quality and risk of bias were also assessed.ResultsFour exploratory studies were included in reporting GEA of either tissues attached to SLA or SLActive implants after 4 to 14 days or cells attached to TiOBlast or Osseospeed implants after 3 to 7 days. A total of 111 implants from 43 patients were analyzed using validated array methods; however, considerable heterogeneity and risk of bias were detected. A consistent overall pattern of gene expression was observed; genes representing an immuno-inflammatory response were overexpressed at days 3 to 4, followed by genes representing osteogenic processes at day 7. Genes representing bone remodeling, angiogenesis, and neurogenesis were expressed concomitantly with osteogenesis. Several regulators of these processes, such as cytokines, growth factors, transcription factors, and signaling pathways, were identified. Implant surface properties seemed to influence the healing processes at various stages via differential gene expression.ConclusionLimited evidence from gene expression studies in humans indicates that osteogenic processes commence within the first post-operative week and they appear influenced at various stages by implant surface properties.

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“…The canonical Wnt and Smad-dependent BMP pathways interact together and play important roles in osteogenesis [20, 22–26]. However, the crosstalk between those signaling pathways during MSC-mediated regeneration remains elusive.…”

Section: Discussionmentioning

confidence: 99%

Polycaprolactone nanofiber scaffold enhances the osteogenic differentiation potency of various human tissue-derived mesenchymal stem cells

Xue

Qian

et al. 2017

Stem Cell Res Ther

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BackgroundPolycaprolactone (PCL) has been regarded as a promising synthetic material for bone tissue engineering application. Owing to its unique biochemical properties and great compatibility, PCL fibers have come to be explored as a potential delivering scaffold for stem cells to support bone regeneration during clinical application.MethodsThe human derived mesenchymal stem cells (MSCs) were obtained from umbilical cord (UC), bone marrow (BM), and adipose tissue (AD), respectively. The osteogenic differentiation potency of various human MSCs on this novel synthetic biomaterial was also investigated in vitro.ResultsHere, we illustrated that those human UC-, BM-, and AD-derived MSCs exhibited fibroblast-like morphology and expressed characteristic markers. Impressively, PCL nanofiber scaffold could support those MSC adhesion and proliferation. Long-term culture on PCL nanofiber scaffold maintained the viability as well as accelerated the proliferation of those three different kinds of human MSCs. More importantly, the osteogenic differentiation potency of those human MSCs was increased significantly by culturing on PCL nanofiber scaffold. Of note, BM-derived MSCs demonstrated greater differentiation potency among the three kinds of MSCs. The Wnt/β-catenin and Smad3 signaling pathways contributed to the enhanced osteogenesis of human MSCs, which was activated consistently by PCL nanofiber scaffold.ConclusionsThe utilization of PCL nanofiber scaffold would provide a great application potential for MSC-based bone tissue repair by enhancing the osteogenic differentiation of human MSCs.

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Control of osteogenesis by the canonical Wnt and BMP pathways in vivo

Cited by 68 publications

References 109 publications

Human Periosteal Derived Stem Cell Potential: The Impact of age

Human Periosteal Derived Stem Cell Potential: The Impact of age

Genomic analyses of early peri-implant bone healing in humans: a systematic review

Polycaprolactone nanofiber scaffold enhances the osteogenic differentiation potency of various human tissue-derived mesenchymal stem cells

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