2014
DOI: 10.1089/ten.tec.2013.0370
|View full text |Cite
|
Sign up to set email alerts
|

Control of Perfusable Microvascular Network Morphology Using a Multiculture Microfluidic System

Abstract: The mechanical and biochemical microenvironment influences the morphological characteristics of microvascular networks (MVNs) formed by endothelial cells (ECs) undergoing the process of vasculogenesis. The objective of this study was to quantify the role of individual factors in determining key network parameters in an effort to construct a set of design principles for engineering vascular networks with prescribed morphologies. To achieve this goal, we developed a multiculture microfluidic platform enabling pr… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

15
238
2

Year Published

2014
2014
2020
2020

Publication Types

Select...
7
2

Relationship

2
7

Authors

Journals

citations
Cited by 211 publications
(255 citation statements)
references
References 45 publications
15
238
2
Order By: Relevance
“…In contrast to our results, recent in vitro vasculogenesis assays using spatially separated cocultures did not describe recruitment and direct contact of supporting cells with microvessels. 10,11 This might be due to the shorter experimental duration used in these studies. It is also possible that the primary cells used here are more migratory and physiologically relevant as compared to the lung fibroblasts reported earlier.…”
Section: Discussionmentioning
confidence: 97%
See 1 more Smart Citation
“…In contrast to our results, recent in vitro vasculogenesis assays using spatially separated cocultures did not describe recruitment and direct contact of supporting cells with microvessels. 10,11 This might be due to the shorter experimental duration used in these studies. It is also possible that the primary cells used here are more migratory and physiologically relevant as compared to the lung fibroblasts reported earlier.…”
Section: Discussionmentioning
confidence: 97%
“…Yet another method exploited the natural microvessel formation of endothelial cells inside fibrin gel by providing appropriate growth factors and signals from fibroblasts. 10,11 These novel models mimic the cellular microenvironment much better than standard in vitro assays performed in static conditions. Endothelial cells attach to and modify a threedimensional (3D) meshwork to form perfusable microvessels and with the addition of perivascular cells or physiological interstitial flow further approach in vivo-like conditions.…”
Section: Introductionmentioning
confidence: 99%
“…26 These self-organized, perfusable 3D mVNs were recently used to better understand extravasation events in tumor cell metastasis. 27 The in vitro microvascular network platform is a very useful tool for systematically studying transport of microparticles in real time, with increased physiological relevance compared to microuidics-based ow assays (similar to other bio-inspired experiments 4,28 ).…”
Section: Introductionmentioning
confidence: 99%
“…Hydrogel-incorporating microfluidic devices have been developed to investigate the morphogenetic nature of blood vessels, angiogenesis (Chung et al 2009b;Shin et al 2011Shin et al , 2012 Jeong et al 2011a, b;Munn 2011), and vasculogenesis (Kim et al 2013;Whisler et al 2014;Chen et al 2013). Angiogenesis denotes the formation of new blood vessels from preexisting vessels, and vasculogenesis is the process that occurs when there are no preexisting vessels.…”
Section: D Angiogenesis Assaymentioning
confidence: 99%