Control of photosystem genes in Rhodobacter capsulatus

Bauer, Carl E.; Buggy, Joseph J.; Mosley, Chere S.

doi:10.1016/0168-9525(93)90188-n

Cited by 57 publications

(37 citation statements)

References 36 publications

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“…We recently described a cluster of regulatory genes in R. capsulatus that control expression of the puf, puh, and puc operons in response to changes in environmental growth conditions. Two of the regulatory loci, regB and regA, code for a sensor kinase and partner response regulator, respectively, that control puf, puh, and puc operon expression in response to anaerobiosis (4,26,34,43). A third regulatory locus, hvrA, codes for a trans-acting factor that controls light-mediated regulation of puh and puf expression (10).…”

Section: Resultsmentioning

confidence: 99%

Cloning and characterization of senC, a gene involved in both aerobic respiration and photosynthesis gene expression in Rhodobacter capsulatus

Buggy

Bauer

1995

J Bacteriol

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The purple nonsulfur photosynthetic eubacterium Rhodobacter capsulatus is a versatile organism that can obtain cellular energy by several means, including the capture of light energy for photosynthesis as well as the use of light-independent respiration, in which molecular oxygen serves as a terminal electron acceptor. In this study, we have identified and characterized a novel gene, senC, mutations in which affect respiration as well as the induction of photosynthesis gene expression. The protein coded by senC exhibits 33% sequence identity to the yeast nucleus-encoded protein SCO1, which is thought to be a mitochondrion-associated cytochrome c oxidase assembly factor. Like yeast SCO1, SenC is required for optimal cytochrome c oxidase activity in aerobically grown R. capsulatus cells. We further show that senC is required for maximal induction from the puf and puh operons, which encode the structural polypeptides of the light-harvesting and reaction center complexes.

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Section: Resultsmentioning

confidence: 99%

Cloning and characterization of senC, a gene involved in both aerobic respiration and photosynthesis gene expression in Rhodobacter capsulatus

Buggy

Bauer

1995

J Bacteriol

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“…The incident light intensity is also known to modulate PS gene expression. Recently, substantial progress has been achieved in uncovering some of the molecular species involved in the regulatory network governing PS gene expression (3,7,22).…”

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Genetic evidence that PpsR from Rhodobacter sphaeroides 2.4.1 functions as a repressor of puc and bchF expression

1995

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“…This pattern of oxygen and light regulation is, in general, repeated at the level of gene transcription (4,28,29). While progress has been made in uncovering the transcriptional regulatory mechanisms governing expression of the puf, puh, andpuc operons (4, 28, 29, 31, 43, 49), little is known about the more weakly regulated bch and crt operons.…”

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confidence: 99%

Sequencing, chromosomal inactivation, and functional expression in Escherichia coli of ppsR, a gene which represses carotenoid and bacteriochlorophyll synthesis in Rhodobacter sphaeroides

1994

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Sequencing of a DNA fragment that causes trans suppression of bacteriochlorophyll and carotenoid levels in Rhodobacter sphaeroides revealed two genes: orf-192 and ppsR. The ppsR gene alone is sufficient for photopigment suppression. Inactivation of the R. sphaeroides chromosomal copy of ppsR results in overproduction of both bacteriochlorophyll and carotenoid pigments. The deduced 464-amino-acid protein product of ppsR is homologous to the CrtJ protein of Rhodobacter capsulatus and contains a helix-turn-helix domain that is found in various DNA-binding proteins. Removal of the helix-turn-helix domain renders PpsR nonfunctional. The promoter of ppsR is located within the coding region of the upstream orf-192 gene. When this promoter is replaced by a lacZ promoter, ppsR is expressed in Escherichia coli. An R. sphaeroides DNA fragment carrying crtD', -E, and -F and bchC, -X, -Y, and -Z' exhibited putative promoter activity in E. coli. This putative promoter activity could be suppressed by PpsR in both E. coli and R. sphaeroides. These results suggest that PpsR is a transcriptional repressor. It could potentially act by binding to a putative regulatory palindrome found in the 5' flanking regions of a number of R. sphaeroides and R. capsulatus photosynthesis genes.Rhodobacter sphaeroides is a metabolically versatile gramnegative bacterium which is able to grow by aerobic and anaerobic respiration, fermentation, and anoxygenic photosynthesis. Its photosynthetic apparatus is relatively simple, consisting of two types of light-harvesting antennae (LH-I and LH-II), a reaction center (RC) complex, and various electron transport proteins. Bacteriochlorophyll (Bch) and carotenoid (Crt) photopigments are noncovalently bound to the LH-I, LH-II, and RC complexes (28

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Control of photosystem genes in Rhodobacter capsulatus

Cited by 57 publications

References 36 publications

Cloning and characterization of senC, a gene involved in both aerobic respiration and photosynthesis gene expression in Rhodobacter capsulatus

Cloning and characterization of senC, a gene involved in both aerobic respiration and photosynthesis gene expression in Rhodobacter capsulatus

Genetic evidence that PpsR from Rhodobacter sphaeroides 2.4.1 functions as a repressor of puc and bchF expression

Sequencing, chromosomal inactivation, and functional expression in Escherichia coli of ppsR, a gene which represses carotenoid and bacteriochlorophyll synthesis in Rhodobacter sphaeroides

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