Control of postharvest green mould of citrus by haliscosamine isolated from Haliclona viscosa sponge

Abstract

The aim of this study was to evaluate the antifungal activity of haliscosamine isolated from Haliclona viscosa sea sponge from the Moroccan Atlantic coast against Penicillium digitatum, the causal agent of citrus green mould. The in vitro antifungal activity of haliscosamine, determined using the agar disc-diffusion method, showed that haliscosamine and DESOGERME SP VEGETAUX â (reference product) have fungicidal activity with inhibition diameters of 19 and 18 mm, respectively. Haliscosamine and a positive control (DESO-GERME SP VEGETAUX â ) were tested in vivo in comparison with a negative control on orange fruits in normal storage conditions. This test showed that after four weeks of storage, the rate of decay of citrus fruits treated with haliscosamine or DESOGERME does not exceed 14%, whereas in the negative control the decay rate is 77%. The results of in vitro and in vivo tests indicate that haliscosamine and DESOGERME SP VEGETAUX â have an important antifungal potential against P. digitatum and that haliscosamine (a natural product) may represent an alternative to the use of chemical fungicides.

Activity of Haliscosamine against Fusarium oxysporum f.sp. melonis: in vitro and in vivo analysis

“…Haliscosamine isolated from Haliclona viscosa sponge, has a strong antifungal activity with a wide spectrum. It is active against human pathogenic yeasts ( Candida albicans ATCC 10231, Candida tropicalis R2 CIP 1276.81 and Cryptococcus neoformans ATCC 11576) (El Amraoui et al 2013 ) and against a very resistant phytopathogenic fungus ( Penicilium digitatum ) (EL Amraoui et al 2014a ).…”

“…DESOGERME SP VEGETAUX® (LAKORALE, Morocco), used in this study as a positive control, is an algaecide, fungicide and bactericide product used in Morocco both to remove algae, fungi and bacteria in irrigation systems and also to disinfect soil. It consists of 20 g/L of polyhexamethyle bioguanidine hydrochlorique and 50 g/L of N-alkyl dimethyl benzyl ammonium chloride (EL Amraoui et al 2014a ).…”

“…Furthermore, Marine sponges are a potential source of new biological compounds with diverse biological activities (Acosta and Rodriguez 1992 ; Baslow and Turlapaty 1969 ; Akiyama et al 2009 ; Bao et al 2007a ; Bao et al 2005 ; Bao et al 2007b ). In Morocco, few studies are carried out about Moroccan sponges with an important biological material for the isolation of new molecule (El Amraoui et al 2014b ; EL Amraoui et al 2014a ; El Amraoui et al 2013 ; El Amraoui et al 2010 ; El-Wahidi et al 2011 ; El-Wahidi et al 2013 ). Haliscosamine isolated from the Moroccan marine sponge Haliclona viscosa is a new derivative of sphingosine with an original molecular structure ((Z)-2-amino-docos-9- ene-1,3,13,14-tetraol) (El Amraoui et al 2013 ).…”