Control of reservoir souring by incomplete nitrate reduction in Indian oil fields

Prajapat, Ganshyam; Jain, Shikha; Lal, Banwari; Lavania, Meeta; Agrawal, Akhil

doi:10.1016/j.biteb.2022.101302

Cited by 2 publications

(4 citation statements)

References 41 publications

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“…It is noteworthy that eight studies do not specify the use of RNA preservatives (Table 3). Furthermore, two studies [19,46] did not specify preprocessing and nucleic acid preservative agents (Table 3).…”

Section: Methods Of Preprocessing Preservation and Extraction Of Rnamentioning

confidence: 99%

“…The pair of primers 341F (5 -CCTAYGGGRBGCASCAG-3 ) and 806R (5 -GGACTACNNGGGTATCTAAT-3 ) were used in four of the eight studies that sequenced 16S rRNA gene (Table 4). It should be highlighted that in one study [46], isolated RNA from PW and IW samples was used solely for quantitative PCR (qPCR) based on the 16S rRNA gene (EUB341F-EUB534R) and functional genes narG (narG1575F/1748R) and nirS (nirS1189F/1376R) (Table 4).…”

Section: Methods For Amplification and Sequencing Rnamentioning

confidence: 99%

“…Different types of environmental samples were evaluated, including produced water (PW), injection water (IW), solid deposits (SD), oil (OIL), and oily sludge (OS) (Table 2). The PW, IW, and OIL samples were mostly collected from production and injection wells , and surface pipelines [1,3,4,15,[17][18][19]43,[44][45][46]. The OS samples were obtained from storage tanks and fluid passages originating from oil reservoirs [13,14], while the DS was acquired from the internal scraping of pipelines [1].…”

Section: Included Studiesmentioning

confidence: 99%

See 2 more Smart Citations

Metabolically Active Microbial Communities in Oilfields: A Systematic Review and Synthesis of RNA Preservation, Extraction, and Sequencing Methods

Gomes,

García,

Dutra

et al. 2023

Applied Microbiology

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Characterizing metabolically active microorganisms using RNA-based methods is a crucial tool for monitoring and mitigating operational issues, such as oil biodegradation and biocorrosion of pipelines in the oil and gas industry. Our review, a pioneering study, addresses the main methods used to preserve, isolate, and sequence RNA from oilfield samples and describes the most abundant metabolically active genera studied. Using the MEDLINE/PubMed, PubMed Central, Scopus, and Web of Science databases, 2.561 potentially eligible records were identified. After screening, 20 studies were included in our review, underscoring the scarcity of studies related to the subject. Data were extracted and reported according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA). These studies evaluated different samples, including produced water (PW), injection water (IW), solid deposits (SD), oil (OIL), and oily sludge (OS) collected from oilfields located in Australia, China, India, Mexico, and the United Arab Emirates. Environmental samples accounted for 55% of the studies, while enriched cultures and microbial consortia represented 35% and 15% of studies, respectively. PW was the most frequently studied sample, comprising 72% of all samples. Filtration and centrifugation were the only processes employed to concentrate the biomass present in samples. For RNA preservation, the most used method was a solution composed of 95:5 v/v ethanol/TRIzol, while for RNA isolation, the TRIzol reagent was the most cited. The Sanger sequencing method was used in all studies evaluating functional genes (alkB, dsrA, aprA, assA, and mcrA), and the Next-Generation Sequencing (NGS) method was employed in studies for sequencing transcripts of the 16S rRNA gene and metatranscriptomes. Pseudomonas (16S rRNA = PW: 2%; IW: 8%; metatranscriptome = PW: 20%) and Acinetobacter (16S rRNA = PW: 1%; IW: 4%; metatranscriptome = PW: 17%) were the most abundant genera. This study outlined the primary methods employed in researching metabolically active microorganisms. These data provide a foundation for future research. However, it is essential to note that we cannot yet determine the most effective method. We hope that this study will inspire further research related to the standardization of RNA preservation, extraction, and sequencing methods and significantly contribute to our understanding of active microbial communities in oilfields.

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Section: Methods Of Preprocessing Preservation and Extraction Of Rnamentioning

confidence: 99%

Section: Methods For Amplification and Sequencing Rnamentioning

confidence: 99%

Section: Included Studiesmentioning

confidence: 99%

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Metabolically Active Microbial Communities in Oilfields: A Systematic Review and Synthesis of RNA Preservation, Extraction, and Sequencing Methods

Gomes,

García,

Dutra

et al. 2023

Applied Microbiology

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“…Because of its low cost, easy availability, and high solubility in water phases, it had been claimed as a preferred solution over the use of biocides. A high number of studies report its field application, e.g., [14,22,56,105]. The proposed mechanisms for reducing H2S formation through nitrate or nitrite modification include the following: (1) the preferential use of nitrate as an electron acceptor instead of sulfate by some species of SRB, thus suppressing microbial sulfate reduction; (2) suppression of SRB activity as a result of competition between heterotrophic NRB and SRB for the same electron donors with SRB being out-competed; and (3) inhibition of SRB activity via intermediate compounds generated during nitrate reduction by heterotrophic NRB or NR-SOB (nitrate-reducing, sulfide-oxidizing bacteria), e.g., NO, N2O, and, especially, nitrite, NO2 -.…”

Section: Nitrate/nitritementioning

confidence: 99%

Assessment of the Biogenic Souring in Oil Reservoirs under Secondary and Tertiary Oil Recovery

Alkan,

Kögler,

Namazova

et al. 2024

Energies

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The formation of hydrogen sulfide (H2S) in petroleum reservoirs by anaerobic microbial activity (through sulfate-reducing microorganisms, SRMs) is called biogenic souring of reservoirs and poses a risk in the petroleum industry as the compound is extremely toxic, flammable, and corrosive, causing devastating damage to reservoirs and associated surface facilities. In this paper, we present a workflow and the tools to assess biogenic souring from a pragmatic engineering perspective. The retention of H2S in the reservoir due to the reactions with iron-bearing rock minerals (e.g., siderite) is shown in a theoretical approach here and supported with literature data. Cases are provided for two fields under secondary (waterflooding) and tertiary flooding with microbial enhanced oil recovery (MEOR). The use of the Monte Carlo method as a numerical modeling tool to incorporate uncertainties in the measured physical/chemical/biochemical data is demonstrated as well. A list of studies conducted with different chemicals alone or in combination with various biocides to mitigate biogenic souring provides an overview of potential inhibitors as well as possible applications. Furthermore, the results of static and dynamic inhibition tests using molybdate are presented in more detail due to its promising mitigation ability. Finally, a three-step workflow for the risk assessment of biogenic souring and its possible mitigation is presented and discussed.

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Control of reservoir souring by incomplete nitrate reduction in Indian oil fields

Cited by 2 publications

References 41 publications

Metabolically Active Microbial Communities in Oilfields: A Systematic Review and Synthesis of RNA Preservation, Extraction, and Sequencing Methods

Metabolically Active Microbial Communities in Oilfields: A Systematic Review and Synthesis of RNA Preservation, Extraction, and Sequencing Methods

Assessment of the Biogenic Souring in Oil Reservoirs under Secondary and Tertiary Oil Recovery

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