Control of rRNA transcription in Escherichia coli.

Condon, C; Squires, Catherine L.

doi:10.1128/mmbr.59.4.623-645.1995

Cited by 238 publications

(164 citation statements)

References 280 publications

(432 reference statements)

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“…B. subtilis has 10 rrn operons, but only two types [15]. E. coli has seven rrn operons, but only two types [16]. The variation was comparable to that of V. cholerae.…”

Section: Resultsmentioning

confidence: 88%

“…An intergenic spacer without a tRNA gene has been reported from Bacillus subtilis [15]. E. coli has two di¡erent types of spacer : one contains the tRNA Glu gene, while the other contains the tRNA Ile and tRNA Ala genes [16]. A spacer encoding tRNA Glu , tRNA Lys and tRNA Val has previously only been found in V. cholerae and V. mimicus [10,17].…”

Section: Resultsmentioning

confidence: 99%

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Structural variation in the 16S–23S rRNA intergenic spacers of Vibrio parahaemolyticus

Maeda¹

2000

FEMS Microbiology Letters

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The 16S^23S rRNA intergenic spacers (IGS) of Vibrio parahaemolyticus were PCR-amplified and cloned with pT7Blue T vector. A total of six clones were isolated dependent on size difference. The clones were different with respect to both the number and the composition of the tRNA genes included, and were designated IGS-0, IGS-E, IGS-IA, IGS-AE, IGS-EKV and IGS-EKAV. IGS-EKAV included the cluster of tRNA Glu -tRNA Lys -tRNA Ala -tRNA Val ; IGS-EKV, tRNA Glu -tRNA Lys -tRNA Val ; IGS-AE, tRNA Ala -tRNA Glu ; IGS-IA, tRNA Ile -tRNA Ala ; and IGS-E, tRNA Glu . IGS-0 had no tRNA gene. Some similarities were found in the nucleotide sequence of the non-coding regions flanked by the tRNA genes. The structure difference found in the spacers is meaningful for elucidating the evolutionary line of each ribosomal RNA operon and the profile is applicable as a molecular marker of the bacterium. ß

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“…B. subtilis has 10 rrn operons, but only two types [15]. E. coli has seven rrn operons, but only two types [16]. The variation was comparable to that of V. cholerae.…”

Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Structural variation in the 16S–23S rRNA intergenic spacers of Vibrio parahaemolyticus

Maeda¹

2000

FEMS Microbiology Letters

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“…S2) possesses the conserved domains D1, D1′, D2, D3 and D4, that are required for correct folding of the rRNA transcript. The antiterminator box A, a motif of 12 nucleotides, that is essential for maintaining transcription of the rRNA genes in the correct stoichiometry (Berg et al, 1989;Condon et al, 1995), is immediately adjacent to domain D4 at the base of the 23S rRNA processing stem (Fig. 6).…”

Section: Internal Transcribed Spacer Its-l and Its-s: Comparison Of Cmentioning

confidence: 99%

Characterization of true‐branching cyanobacteria from geothermal sites and hot springs of Costa Rica

Finsinger

Scholz

Serrano

et al. 2007

Environmental Microbiology

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Costa Rica is at the centre of the Mesoamerican biodiversity hotspot. Little is known about cyanobacteria from this region so far. Here, four isolates of the order Stigonematales (section V) were characterized in a polyphasic approach. All strains were isolated from geothermal sites and hot springs of Costa Rica. However, one of them, identified as Westiellopsis sp. Ar73, did not grow at more than 40 degrees C. Based on its identical 16S rRNA to several previously isolated Westiellopsis sp. and Fischerella muscicola strains, a ubiquitous distribution throughout tropical and subtropical regions can be implied. In contrast, the isolates MV9, MV11 and RV14 grew well up to 50-55 degrees C. Based on morphologic, ultrastructural, molecular and physiologic data, MV9, MV11 and RV14 were identified to belong to the genus Fischerella. Two distinct intergenic transcribed spacer (ITS) types, with or without tRNA genes, were detected for all Stigonematales analysed here, indicating ITS polymorphism as a characteristic feature of heterocystous cyanobacteria. In phylogenetic trees, these Fischerella spp. formed a new and distinct clade within the wider lineage of thermophilic Fischerella (Mastigocladus cf. laminosus), which might represent a geographic lineage. Thus, geographic isolation may be an underestimated aspect of microbial evolution. The strains presented here are suitable as new models to study this group of cyanobacteria.

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“…Such an association raises a question as to what regulates Rho outside ORFs (notably during transcription of long leader regions) when there is no ribosome trailing behind RNAP to increase its processivity (Proshkin et al, 2010) nor to prevent the mRNA from associating with Rho (Roberts et al, 2008;Rabhi et al, 2010). One possibility is that, as observed during transcription of ribosomal rrn operons or bacteriophage l chromosome (reviewed in Condon et al, 1995;Roberts et al, 2008;Sen et al, 2008), accessory factors bind, in a conditional manner, to RNA and/or to the transcription machinery to form an antitermination complex resistant to Rho. Antitermination complexes that resist to Rho-dependent termination signals within rrn and l operons contain the NusA, NusB, NusE (ribosomal protein S10), and NusG factors as well as specific factors such as bacteriophage N protein for lN antitermination or ribosomal proteins (S2, S4, L1, L3, L4, and L13) and other unidentified cellular factor(s) for rrn antitermination (Condon et al, 1995;Torres et al, 2001;Roberts et al, 2008;Sen et al, 2008). Both rrn and lN antitermination complexes are stabilized by elaborate networks of interactions among their many components that include binding of a NusB-NusE heterodimer to a boxA RNA sequence found in the rrn and l transcripts (Roberts et al, 2008;Sen et al, 2008) and binding of NusG to RNAP through its N-terminal domain (NusG-NTD) and to NusE through its C-terminal domain (NusG-CTD; Mooney et al, 2009b;Burmann et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

The Sm-like RNA chaperone Hfq mediates transcription antitermination at Rho-dependent terminators

et al. 2011

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In Escherichia coli, the essential motor protein Rho promotes transcription termination in a tightly controlled manner that is not fully understood. Here, we show that the general post-transcriptional regulatory protein Hfq associates with Rho to regulate Rho function. The Hfq:Rho complex can be further stabilized by RNA bridging both factors in a configuration that inhibits the ATP hydrolysis and duplex unwinding activities of Rho and that mediates transcription antitermination at Rho-dependent terminators in vitro and in vivo. Antitermination at a prototypical terminator (ltR1) requires Hfq binding to an A/U-rich transcript region directly upstream from the terminator. Antitermination is modulated by trans-acting factors (NusG or nucleic acid competitors) that affect Hfq association with Rho or RNA. These data unveil a new Hfq function and a novel transcription regulatory mechanism with potentially important implications for bacterial RNA metabolism, gene silencing, and pathogenicity.

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Control of rRNA transcription in Escherichia coli.

Cited by 238 publications

References 280 publications

Structural variation in the 16S–23S rRNA intergenic spacers of Vibrio parahaemolyticus

Structural variation in the 16S–23S rRNA intergenic spacers of Vibrio parahaemolyticus

Characterization of true‐branching cyanobacteria from geothermal sites and hot springs of Costa Rica

The Sm-like RNA chaperone Hfq mediates transcription antitermination at Rho-dependent terminators

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