Control of self-assembling oligopeptide matrix formation through systematic variation of amino acid sequence

Caplan, Michael R.; Schwartzfarb, Elissa M.; Zhang, Shuguang; Kamm, Roger D.; Lauffenburger, Douglas A.

doi:10.1016/s0142-9612(01)00099-0

Cited by 256 publications

(262 citation statements)

References 13 publications

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“…This approach would obviate the need for biotin-streptavidin complexes because the protein could be covalently linked to the peptide. However, it is possible that entropy or steric effects could limit fibril growth (24) and by implication, incorporation of fusion proteins into selfassembling peptide fibrils. In addition, the biotin sandwich approach can be considered a modular building-block strategy that could be used to multifunctionalize an injectable microenvironment.…”

Section: Discussionmentioning

confidence: 99%

Local myocardial insulin-like growth factor 1 (IGF-1) delivery with biotinylated peptide nanofibers improves cell therapy for myocardial infarction

Davis

Hsieh²,

Takahashi³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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563

411

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Strategies for cardiac repair include injection of cells, but these approaches have been hampered by poor cell engraftment, survival, and differentiation. To address these shortcomings for the purpose of improving cardiac function after injury, we designed self-assembling peptide nanofibers for prolonged delivery of insulin-like growth factor 1 (IGF-1), a cardiomyocyte growth and differentiation factor, to the myocardium, using a ''biotin sandwich'' approach. Biotinylated IGF-1 was complexed with tetravalent streptavidin and then bound to biotinylated self-assembling peptides. This biotin sandwich strategy allowed binding of IGF-1 but did not prevent self-assembly of the peptides into nanofibers within the myocardium. IGF-1 that was bound to peptide nanofibers activated Akt, decreased activation of caspase-3, and increased expression of cardiac troponin I in cardiomyocytes. After injection into rat myocardium, biotinylated nanofibers provided sustained IGF-1 delivery for 28 days, and targeted delivery of IGF-1 in vivo increased activation of Akt in the myocardium. When combined with transplanted cardiomyocytes, IGF-1 delivery by biotinylated nanofibers decreased caspase-3 cleavage by 28% and increased the myocyte cross-sectional area by 25% compared with cells embedded within nanofibers alone or with untethered IGF-1. Finally, cell therapy with IGF-1 delivery by biotinylated nanofibers improved systolic function after experimental myocardial infarction, demonstrating how engineering the local cellular microenvironment can improve cell therapy.engineering ͉ maturation ͉ scaffold

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Section: Discussionmentioning

confidence: 99%

Local myocardial insulin-like growth factor 1 (IGF-1) delivery with biotinylated peptide nanofibers improves cell therapy for myocardial infarction

Davis

Hsieh²,

Takahashi³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

563

411

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“…Zhang et al have synthesized short oligopeptides having 12 to 16 amino acids that spontaneously form stable β-sheet structures under physiological solution conditions [220][221][222][223][224][225][226][227][228][229][230][231][232][233][234][235]. As shown in Figure 19a, these ionic selfcomplementary oligopeptides have amphiphilic character; one face of the molecule consists of nonpolar, hydrophobic amino acids (such as Ala, Phe, or Leu), and the other face consists of alternating oppositely charged amino acids (such as positively charged Lys or Arg and negatively charged Asp or Glu).…”

Section: β-Sheet Forming Ionic Oligopeptidesmentioning

confidence: 99%

Peptide-based biopolymers in biomedicine and biotechnology

Chow

Nunalee

Lim

et al. 2008

Materials Science and Engineering: R: Reports

286

231

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Peptides are emerging as a new class of biomaterials due to their unique chemical, physical, and biological properties. The development of peptide-based biomaterials is driven by the convergence of protein engineering and macromolecular self-assembly. This review covers the basic principles, applications, and prospects of peptide-based biomaterials. We focus on both chemically synthesized and genetically encoded peptides, including poly-amino acids, elastin-like polypeptides, silk-like polymers and other biopolymers based on repetitive peptide motifs. Applications of these engineered biomolecules in protein purification, controlled drug delivery, tissue engineering, and biosurface engineering are discussed.

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“…4, 23, 24, 25, 26, 27, 28, 29 β ‐sheet peptides are of particular interest as they allow the fabrication of very stable hydrogels with properties that can be tailored through peptide design, media properties and processing. We have recently investigated the self‐assembly and gelation properties of a family of β ‐sheet peptides 30, 31, 32, 33, 34 based on the design developed by Zhang and co‐workers.…”

Section: Introductionmentioning

confidence: 99%

Peptide hydrogel in vitro non‐inflammatory potential

Markey

Workman

Bruce

et al. 2016

Journal of Peptide Science

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Peptide‐based hydrogels have attracted significant interest in recent years as these soft, highly hydrated materials can be engineered to mimic the cell niche with significant potential applications in the biomedical field. Their potential use in vivo in particular is dependent on their biocompatibility, including their potential to cause an inflammatory response. In this work, we investigated in vitro the inflammatory potential of a β‐sheet forming peptide (FEFEFKFK; F: phenylalanine, E: glutamic acid; K: lysine) hydrogel by encapsulating murine monocytes within it (3D culture) and using the production of cytokines, IL‐β, IL‐6 and TNFα, as markers of inflammatory response. No statistically significant release of cytokines in our test sample (media + gel + cells) was observed after 48 or 72 h of culture showing that our hydrogels do not incite a pro‐inflammatory response in vitro. These results show the potential biocompatibility of these hydrogels and therefore their potential for in vivo use. The work also highlighted the difference in monocyte behaviour, proliferation and morphology changes when cultured in 2D vs. 3D. © 2016 The Authors Journal of Peptide Science published by European Peptide Society and John Wiley & Sons Ltd.

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Control of self-assembling oligopeptide matrix formation through systematic variation of amino acid sequence

Cited by 256 publications

References 13 publications

Local myocardial insulin-like growth factor 1 (IGF-1) delivery with biotinylated peptide nanofibers improves cell therapy for myocardial infarction

Local myocardial insulin-like growth factor 1 (IGF-1) delivery with biotinylated peptide nanofibers improves cell therapy for myocardial infarction

Peptide-based biopolymers in biomedicine and biotechnology

Peptide hydrogel in vitro non‐inflammatory potential

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