Control of sheep gastrointestinal nematodes using the combination of Duddingtonia flagrans and Levamisole Hydrochloride 5%

Vilela, Vinícius Longo Ribeiro; Feitosa, Thaís Ferreira; Braga, Fábio Ribeiro; Vieira, Vanessa Diniz; Lucena, Samuel Cavalcante de; Araújo, Jackson Víctor de

doi:10.1590/s1984-296120180011

Cited by 23 publications

(19 citation statements)

References 29 publications

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“…The D. flagrans group showed low EPG values throughout the experiment (p < 0.05), with the parasite load on day 120 being 92% lower than that in the control group. A reduction in small ruminant EPG, although lower than that noted in the present study, was observed by Vilela et al (2012), Vilela et al (2013), Vilela et al (2016) and Vilela et al (2018) after the use of pellets of nematophagous fungi in the Northeastern semiarid region. However, these animals were kept in an extensive regime and fed on pasture native to the Caatinga, which increased reinfection rates.…”

Section: Discussioncontrasting

confidence: 85%

Use of Duddingtonia flagrans in the control of gastrointestinal nematodes of feedlot goats

Vilela

Feitosa

Braga

et al. 2020

SCA

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This study aimed to evaluate the use of a sodium alginate matrix-pelletized formulation of Duddingtonia flagrans for biological control of gastrointestinal nematodiasis in feedlot goats in the semiarid region of Northeastern Brazil. We used 20 Saanen female goats (age, 4 months; average weight, 12 kg) that did not receive anthelmintic treatment and had counting of eggs per gram of faeces (EPGs) ? 500. The animals were divided into two groups: in group 1 (D. flagrans group), each animal received 3 g of pellets (0.6 g of D. flagrans mycelium) per 10 kg of body weight, twice a week, over 4 months; and in group 2 (control group), each animal received 3 g of pellets without fungus per 10 kg of body weight, twice a week, over 4 months. Each group was maintained in a separate 15-m2 stall. Larval cultures and measurements of weight, EPG, and packed cell volume (PCV) were performed every 15 days. We observed low EPG levels in the D. flagrans group throughout the experimental period, with a significant difference (p < 0.05) on day 30 and from day 60, having, at the end of the experiment, average OPG values of only 150, reduction of 92.3% when compared to control group. Haemonchus sp. was the most prevalent helminth in all larval cultures. The D. flagrans group showed a mean weight gain of 8.8 kg at the end of the experiment (p < 0.05), while the control group showed a mean weight gain of 4.8 kg. The best PCV results (p < 0.05) were also observed in the D. flagrans group from day 30. Thus, the use of D. flagrans pellets in a sodium alginate matrix was effective in controlling gastrointestinal nematodiasis of feedlot goats in the semiarid region of Northeastern Brazil.

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Section: Discussioncontrasting

confidence: 85%

Use of Duddingtonia flagrans in the control of gastrointestinal nematodes of feedlot goats

Vilela

Feitosa

Braga

et al. 2020

SCA

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“…Vilela et al . (2018) reported the coadministration of pellets containing D . flagrans and levamisole to control sheep parasites, and levamisole is used as a deworming agent when the EPG of sheep feces exceeds 1500.…”

Section: Resultsmentioning

confidence: 99%

In vitro assays on the susceptibility of four species of nematophagous fungi to anthelmintics and chemical fungicides/antifungal drug

Wang

Zhang

Gong

et al. 2021

Lett Appl Microbiol

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Using nematophagous fungi for the biological control of animal parasitic nematodes will become one of the most promising strategies in the search for alternative chemical drugs. The purpose of this study was to check the in vitro activity of four anthelmintics, four chemical fungicides and two antifungal drugs on the spore germination of nematophagous fungi: Duddingtonia flagrans (SF170), Arthrobotrys oligospora (447), Arthrobotrys superba (435) and Arthrobotrys sp. (PS011). A modified 24-well cell culture plate assay was conducted to evaluate the susceptibility of nematophagous fungi against drugs tested by calculating the effective middle concentrations (EC 50 ) of each tested drug to inhibit the germination of fungal spores. EC 50 ranged between 0Á7 and 47Á2 μg ml −1 for fenbendazole, thiabendazole and ivermectin, except levamisole (546Á5-4057Á8 μg ml −1 ). EC 50 of tested fungicides was 0Á6-2Á3 μg ml −1 for carbendazim, 55Á9-247Á4 μg ml −1 for metalaxyl, 24Á4-45Á2 μg ml −1 for difenoconazole, and 555Á9-1438Á3 μg ml −1 for pentachloronitrobenzene (PCNB). EC 50 of two antifungal drugs was 0Á03-3Á4 μg ml −1 for amphotericin B and 0Á3-10Á9 μg ml −1 for ketoconazole. The results showed that 10 tested drugs, except for levamisole and PCNB, had in vitro inhibitory effects on nematophagous fungi. The chlamydospores of D. flagrans had the highest sensitivity to nine tested drugs, except for ketoconazole.

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“…The use of biological control by helminthophagous fungi provides an option to reduce the rates of contamination by larvae and, consequently, less reinfection for the animals, enabling them to develop natural immunity against the helminths. In addition, animals that receive helminthophagous fungi have higher weight gain rates, higher globular volume percentages, and lower EPG values when compared to those that do not receive the fungi [5,[67][68][69][70]. In grazing horses that received D. flagrans chlamydospores daily, strongylide counts below 200 EPG were obtained for 16 months, also verifying that they recovered normal blood values of erythrocytes, hemoglobin, and hematocrit [71].…”

Section: Advances In the Control Of Gastrointestinal Nematodes In Pasture Animalsmentioning

confidence: 77%

Recent Advances in the Control of Helminths of Domestic Animals by Helminthophagous Fungi

et al. 2021

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This review describes the advances acquired and proven in the use of helminthophagous fungi in the control of gastrointestinal helminth parasites in domestic animals. Old and well-known premises about parasitic epidemiology and the factors that can interfere with the best performance of biological control are mentioned. Some of the most promising fungi are Duddingtonia flagrans from the predatory fungi group and Pochonia chamydosporia and Mucor circinelloides from the ovicidal fungi group. These fungi produce resistance spores called chlamidospores. Bioverm® and BioWorma®, based on the fungus D. flagrans, are available as commercial. Biotechnological products such as nanoparticles and obtaining primary and secondary metabolites have already been obtained from these fungi. Because they have different mechanisms of action, ovicidal and predatory fungi, when used together, can present a complementary and synergistic action in the biological control of helminths. Therefore, future research in the search for new formulations, the association of fungi from different groups, extraction of new molecules, and nanoparticles of these fungi in the control of helminths in various domestic animals are desired.

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Control of sheep gastrointestinal nematodes using the combination of Duddingtonia flagrans and Levamisole Hydrochloride 5%

Cited by 23 publications

References 29 publications

Use of Duddingtonia flagrans in the control of gastrointestinal nematodes of feedlot goats

Use of Duddingtonia flagrans in the control of gastrointestinal nematodes of feedlot goats

In vitro assays on the susceptibility of four species of nematophagous fungi to anthelmintics and chemical fungicides/antifungal drug

Recent Advances in the Control of Helminths of Domestic Animals by Helminthophagous Fungi

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