1973
DOI: 10.1007/bf00269438
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Control of short leftward transcripts from the immunity and ori regions in induced coliphage lambda

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Cited by 140 publications
(50 citation statements)
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“…To identify the RexB protein, we replaced the natural ribosome-binding site of RexB with an efficient synthetic one (SD8) (Gold and Stormo 1990) and eliminated the promoter, PLIT (Hayes and Szybalski 1973;Landsmann et al 1982), which is located in the carboxyl terminus of the RexA-coding sequence. A polymerase chain reaction (PCR)-amplified DNA molecule corresponding to coding nucleotides 1-392 of the rexB gene was synthesized with upstream Eagl, Sail, and SD8 ribosomebinding sites by using an amino-terminal PCR primer 5'-AAA-AACGGCCGTCCGACTAAACTAAACTAAGGAAGGAAA-AAAAAA TGCCGGAACAGAATCATGCCTGG-3' and a carboxy-terminal primer 3'-GTAGCAAAAGTTAAAGGTTA-ATATACGTAACCTTAGGAAAAAA-5'.…”
Section: Bacteria Phages and Plasmidsmentioning
confidence: 99%
“…To identify the RexB protein, we replaced the natural ribosome-binding site of RexB with an efficient synthetic one (SD8) (Gold and Stormo 1990) and eliminated the promoter, PLIT (Hayes and Szybalski 1973;Landsmann et al 1982), which is located in the carboxyl terminus of the RexA-coding sequence. A polymerase chain reaction (PCR)-amplified DNA molecule corresponding to coding nucleotides 1-392 of the rexB gene was synthesized with upstream Eagl, Sail, and SD8 ribosomebinding sites by using an amino-terminal PCR primer 5'-AAA-AACGGCCGTCCGACTAAACTAAACTAAGGAAGGAAA-AAAAAA TGCCGGAACAGAATCATGCCTGG-3' and a carboxy-terminal primer 3'-GTAGCAAAAGTTAAAGGTTA-ATATACGTAACCTTAGGAAAAAA-5'.…”
Section: Bacteria Phages and Plasmidsmentioning
confidence: 99%
“…Therefore, we have focused our attention on in vitro synthesis from XpL8 DNA of a discrete 4S RNA designated "oop" (19). This RNA, which is transcribed from a point near the origin of X replication (20,21), provides an attractive system for several reasons. First, the nucleotide sequence of the 4S RNA as well as the sequence of Xpga18 for approximately 35 nucleotides beyond the 3' end of the transcript have been determined (22).…”
Section: Introductionmentioning
confidence: 99%
“…1). The genes rexA and rexB can be expressed coordinately with the cI repressor gene from promoters p RM and p RE (5,7,8). However, there is a third promoter, p LIT , that overlaps the region encoding the carboxyl terminus of rexA.…”
mentioning
confidence: 99%
“…However, there is a third promoter, p LIT , that overlaps the region encoding the carboxyl terminus of rexA. Transcription from p LIT results in the synthesis of the lit mRNA, which permits the expression of rexB without that of rexA (5,7,9). This shift from coordinate to discoordinate expression of rexB and rexA implies that rexB may have another function independent of that of rexA (3,5).…”
mentioning
confidence: 99%