Control of the Biofilms Formed by Curli- and Cellulose-Expressing Shiga Toxin-Producing Escherichia coli Using Treatments with Organic Acids and Commercial Sanitizers

Park, Yoen Ju; Chen, Jinru

doi:10.4315/0362-028x.jfp-14-382

Cited by 8 publications

(7 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although sanitizer treatments were effective at reducing significantly the viability and the metabolism of STEC in biofilms, the biofilm matrix either remained or was partly removed depending on the sanitizer (Table 2). These results agree with previous reports (6,7,68,69). Moreover, no significant differences in viability or metabolic activity were seen between seropathotypes or isolates (7).…”

Section: Discussionsupporting

confidence: 82%

“…CF results indicative of curli and/or cellulose production also were variable and are similar to those of other studies (7,8,48). While curli or cellulose production in some STEC isolates was shown to be associated with biofilm formation (6,8,34,(49)(50)(51), these were shown to be nonessential for biofilm formation (7). In our study, no correlation was found between Congo red or calcofluor-positive isolates and biofilm formation or seropathotype (see Fig.…”

Section: Discussionsupporting

confidence: 69%

“…STEC, an important food-borne and waterborne pathogen, and STEC isolates of different origins (cattle, water, food, and human) have been shown to be able to form biofilms (6)(7)(8). Biofilms are thought to increase STEC survival and persistence in hostile environments such as water-or food-processing plants (37).…”

Section: Discussionmentioning

confidence: 99%

“…The biofilm-forming ability of STEC has been investigated under different conditions, using different STEC isolates representing different serotypes and origins (6)(7)(8). Biofilm formation by STEC may be regarded as a survival strategy for this bacterium (9-12).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections

Vogeleer

Tremblay

Jubelin³

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

bForming biofilms may be a survival strategy of Shiga toxin-producing Escherichia coli to enable it to persist in the environment and the food industry. Here, we evaluate and characterize the biofilm-forming ability of 39 isolates of Shiga toxin-producing Escherichia coli isolates recovered from human infection and belonging to seropathotypes A, B, or C. The presence and/or production of biofilm factors such as curli, cellulose, autotransporter, and fimbriae were investigated. The polymeric matrix of these biofilms was analyzed by confocal microscopy and by enzymatic digestion. Cell viability and matrix integrity were examined after sanitizer treatments. Isolates of the seropathotype A (O157:H7 and O157:NM), which have the highest relative incidence of human infection, had a greater ability to form biofilms than isolates of seropathotype B or C. Seropathotype A isolates were unique in their ability to produce cellulose and poly-N-acetylglucosamine. The integrity of the biofilms was dependent on proteins. Two autotransporter genes, ehaB and espP, and two fimbrial genes, z1538 and lpf2, were identified as potential genetic determinants for biofilm formation. Interestingly, the ability of several isolates from seropathotype A to form biofilms was associated with their ability to agglutinate yeast in a mannose-independent manner. We consider this an unidentified biofilmassociated factor produced by those isolates. Treatment with sanitizers reduced the viability of Shiga toxin-producing Escherichia coli but did not completely remove the biofilm matrix. Overall, our data indicate that biofilm formation could contribute to the persistence of Shiga toxin-producing Escherichia coli and specifically seropathotype A isolates in the environment. Shiga toxin-producing Escherichia coli (STEC) strains, including enterohemorrhagic E. coli (EHEC), are food-borne and waterborne human enteric pathogens responsible for infections. They are associated with important public health concerns in developed countries. Symptoms associated with STEC infections range from abdominal cramps and bloody diarrhea to postinfectious complications, such as hemolytic-uremic syndrome (HUS). HUS, a life-threatening complication of STEC infections, is a consequence of Shiga toxin production. According to the Public Health Agency of Canada (PHAC), more than 30,000 cases of STEC infections occur each year in Canada (1). Environmentally, the main reservoir for STEC is cattle. Transmission of STEC to humans typically is associated with the consumption of contaminated food such as undercooked beef, fresh produce, unpasteurized milk, or contaminated drinking water (2), but these pathogens also can be transmitted from person to person or via direct contact with animals or their feces.The 2003 Karmali seropathotype model classifies STEC into seropathotypes based on their reported incidence in human disease, outbreaks, and/or association with the development of severe symptoms in humans (3). Serotypes frequently responsible for hemorrhagic colitis (HC) and HUS, ...

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections

Vogeleer

Tremblay

Jubelin³

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…In addition, Meira et al [14] found that biofilms adhered over 4 log CFU/cm 2 on all surfaces at both temperatures and the cell detachment was 3 log CFU/cm 2 . Park and Chen [15] performed a study to quantify biofilms formed by different STEC strains on polystyrene and stainless-steel surfaces to determine the effectiveness of 2% acetic or lactic acid and industry recommended concentrations of acidic or alkaline sanitizers, at 28°C. Results showed that the alkaline and acidic sanitizers were more effective than those with the organic acids for removing the biofilms.…”

Section: Introductionmentioning

confidence: 99%

Investigating the Effects of Lactic-Citric Acid Blend and Sodium Lauryl Sulfate on the Inhibition of Shiga Toxin-Producing Escherichia coli in a Broth System

Jackson-Davis¹,

Bethel²,

Staley³

et al. 2018

J Nutr Food Sci

View full text Add to dashboard Cite

Food grade organic acids are used to control undesirable microbes in foods and are commonly diluted in water to facilitate application of desired concentrations of antimicrobial agents. However, water is a poor wetting agent for hydrophobic environments. In hydrophobic environments this problem can be circumvented by use of a food grade processing aid. Sodium lauryl sulfate (SLS) is a surfactant that is generally regarded as a safe food additive. The purpose of this study was to investigate the ability of a lactic-citric acid (LCA) blend and SLS to control the growth of Shiga toxin-producing Escherichia coli (STEC) in a broth system. E. coli O157:H7 and a cocktail of non-O157:H7 (O26, O45, O103, O11, O121, O145, O104:H4) strains were evaluated separately (8.0 log CFU/g). A blend solution of LCA at a 2.4% concentration and SLS at concentrations of 0.05%, 0.25%, and 0.5% were evaluated individually and in combination. Samples were plated onto Sorbitol MacConkey agar infused with rifampicin (100 µg/ml) and incubated at 37°C for 24 h. When used alone the treatments showed no individual effects (p>0.05) of the LCA blend or SLS on the control of O157:H7 and the non-O157:H7 cocktail in the BHI broth. Applying the combined SLS (0.05% and 0.5%) and LCA blend (2.4%) in the broth significantly (p<0.01) reduced the non-O157:H7 by 5.5 log CFU/g and 2.9 and 4.6 log CFU/g in O157:H7 stains. Increasing the SLS concentration (0.25%) in LCA blend (2.4%) was more effective (p<0.01) on O157:H7, showing 5 log CFU/g reduction. This work will assist with providing new information on enhancing the wettability and the exposure of pathogens to antimicrobial treatment.

show abstract

Antimicrobial Resistance of Shiga Toxin-Producing Escherichia coli

Chen

2015

Antimicrobial Resistance and Food Safety

View full text Add to dashboard Cite

Control of the Biofilms Formed by Curli- and Cellulose-Expressing Shiga Toxin-Producing Escherichia coli Using Treatments with Organic Acids and Commercial Sanitizers

Cited by 8 publications

References 38 publications

Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections

Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections

Investigating the Effects of Lactic-Citric Acid Blend and Sodium Lauryl Sulfate on the Inhibition of Shiga Toxin-Producing Escherichia coli in a Broth System

Antimicrobial Resistance of Shiga Toxin-Producing Escherichia coli

Contact Info

Product

Resources

About