Control of the neuronal cell attachment by functionality manipulation of diazo-naphthoquinone/novolak photoresist surface

Nicolau, Dan V.; Taguchi, Takahisa; Tanigawa, Hideo

doi:10.1016/0956-5663(96)88089-4

Cited by 17 publications

(13 citation statements)

References 54 publications

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“…Similar formation of adhesive and non-adhesive domains via photolithography process on the classical DNQ/novolak resist was previously reported for neuronal cell culture [27]. In the study diazo-naphthoquinone/novolak photoresist was used.…”

Section: Proliferationmentioning

confidence: 98%

Lithography application of a novel photoresist for patterning of cells

Halberstadt

Gonsalves

2004

Biomaterials

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Section: Proliferationmentioning

confidence: 98%

Lithography application of a novel photoresist for patterning of cells

Halberstadt

Gonsalves

2004

Biomaterials

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“…Patterning proteins on surfaces have multiple applications in the area of biomedical microdevices, such as microarrays (Allison et al 2006 ; Müller and Nicolau 2005 ), lab-on-a-chip (Chin et al 2012 ), biosensors and bioMEMS (Mujahid et al 2013 ), as well in the area of functional studies for cell and tissue development (Kane et al 1999 ; Nicolau et al 1996 ; Nicolau et al 1999a ). The patterning of proteins can be achieved by their immobilisation from solution in contact with surfaces presenting pre-fabricated patterns, using either selective covalent binding (Ivanova et al 2002 ; Lenci et al 2011 ; Nicolau et al 1998 , 1999b ), or more rarely selective adsorption (Lan et al 2005 ; Nicolau et al 1999b ).…”

Section: Introductionmentioning

confidence: 99%

“…μCP has a special place in the panoply of direct deposition methods, because it is capable of very high resolution of printing, at a low cost of ownership, thus making it widely used in exploratory research (Delamarche et al 2003 ; Delamarche et al 1997 ; Kane et al 1999 ; Renault et al 2003 ). These advantages have led to attempts of applying μCP in many various applications, e.g., patterning neuronal cells (Nicolau et al 1996 ; Thiebaud et al 2002 ), fundamental studies of cell viability and growth as a function of available space (Amirpour et al 2001 ; Chen et al 1997 ; Ghosh et al 2008 ; Nicolau et al 1999a ), and various microarray and lab-on-a-chip applications (Didar et al 2012 ; Hoshino et al 2014 ). Protein μCP can be applied to a large variety of substrates such as glass (plain or treated) (Bou Chakra et al 2008 ), SAM-coated gold slides (Lee et al 2008 ), and polymers, such as polystyrene (Bernard et al 1998 ; Bernard et al 2000 ).…”

Section: Introductionmentioning

confidence: 99%

Protein patterning by microcontact printing using pyramidal PDMS stamps

Filipponi

Livingston

Kašpar

et al. 2016

Biomed Microdevices

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Micro-contact printing, μCP, is a well-established soft-lithography technique for printing biomolecules. μCP uses stamps made of Poly(dimethylsiloxane), PDMS, made by replicating a microstructured silicon master fabricated by semiconductor manufacturing processes. One of the problems of the μCP is the difficult control of the printing process, which, because of the high compressibility of PDMS, is very sensitive to minute changes in the applied pressure. This over-sensitive response leads to frequent and/or uncontrollable collapse of the stamps with high aspect ratios, thus decreasing the printing accuracy and reproducibility. Here we present a straightforward methodology of designing and fabricating PDMS structures with an architecture which uses the collapse of the stamp to reduce, rather than enlarge the variability of the printing. The PDMS stamp, organized as an array of pyramidal micro-posts, whose ceiling collapses when pressed on a flat surface, replicates the structure of the silicon master fabricated by anisotropic wet etching. Upon application of pressure, depending on the size of, and the pitch between, the PDMS pyramids, an air gap is formed surrounding either the entire array, or individual posts. The printing technology, which also exhibits a remarkably low background noise for fluorescence detection, may find applications when the clear demarcation of the shapes of protein patterns and the distance between them are critical, such as microarrays and studies of cell patterning.Electronic supplementary materialThe online version of this article (doi:10.1007/s10544-016-0036-4) contains supplementary material, which is available to authorized users.

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“…MicroCP is a form of soft lithography that uses the relief patterns on a master PDMS stamp to form patterns of SAMs of ink on the surface of a substrate through conformal contact [1][2][3][4][5][6]. Because it is capable of very high resolution of printing at a low cost of ownership, it has been widely used in various applications, such as patterning neuronal cells [7,8], fundamental studies of cell viability and growth as a function of available space [9,10], and various microarray and lab-on-a-chip applications [11,12]. However, after this technique became popular various limitations and problems arose, all of which affected patterning and reproducibility.…”

Section: Introductionmentioning

confidence: 99%

Deformation of Pyramidal PDMS Stamps During Microcontact Printing

Jin

Qiao

2016

Journal of Applied Mechanics

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Microcontact printing (MicroCP) is a form of soft lithography that uses the relief patterns on a master polydimethylsiloxane (PDMS) stamp to form patterns of self-assembled monolayers (SAMs) of ink on the surface of a substrate through conformal contact. Pyramidal PDMS stamps have received a lot of attention in the research community in recent years, due to the fact that the use of the pyramidal architecture has multiple advantages over traditional rectangular and cylindrical PDMS stamps. To better understand the dynamic MicroCP process involving pyramidal PDMS stamps, in this paper, numerical studies on frictionless adhesive contact between pyramidal PDMS stamps and transversely isotropic materials are presented. We use a numerical simulation method in which the adhesive interactions are represented by an interaction potential and the surface deformations are coupled by using half-space Green's functions discretized on the surface. It shows that for pyramidal PDMS stamps, the contact area increases significantly with increasing applied load, and thus, this technique is expected to provide a simple, efficient, and low-cost method to create variable two-dimensional arrays of dot chemical patterns for nanotechnology and biotechnology applications. The DMT-type and Johnson–Kendall–Roberts (JKR)-type-to-DMT-type transition regimes have been explored by conducting the simulations using smaller values of Tabor parameters.

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Control of the neuronal cell attachment by functionality manipulation of diazo-naphthoquinone/novolak photoresist surface

Cited by 17 publications

References 54 publications

Lithography application of a novel photoresist for patterning of cells

Lithography application of a novel photoresist for patterning of cells

Protein patterning by microcontact printing using pyramidal PDMS stamps

Deformation of Pyramidal PDMS Stamps During Microcontact Printing

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