Control of the sheep blowfly in Australia and New Zealand – are we there yet?

Sandeman, R.M.; Levot, G. W.; Heath, A. C. G.; James, Paul; Greeff, JC; Scott, M.; Batterham, Philip; Bowles, Vernon M

doi:10.1016/j.ijpara.2014.08.009

Cited by 50 publications

(77 citation statements)

References 123 publications

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“…aegypti [30, 31]. From an environmental perspective, a potential concern would be that the locus or loci responsible for “rescuing” females could sweep to fixation under a sustained fertile release program, similar to the rapid evolution of resistance in the over application of insecticides [33–35]. Consequently, before any fertile field release, it will be necessary to evaluate screwworm from the target population by crossing with a transgenic strain to determine the degree of heterozygous female lethality.…”

Section: Discussionmentioning

confidence: 99%

A transgenic male-only strain of the New World screwworm for an improved control program using the sterile insect technique

et al. 2016

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BackgroundThe New World screwworm, Cochliomyia hominivorax, is a devastating pest of livestock endemic to subtropical and tropical regions of the Western hemisphere. The larvae of this species feed on the tissue of living animals, including man, and can cause death if untreated. Over 60 years ago, the sterile insect technique (SIT) was developed with the aim of eradicating this pest, initially from Florida but subsequently from all of North and Central America. From the outset it was appreciated that SIT would be more efficient if only sterile males were released in the field, but this was not possible until now.ResultsHere, we report on the development and evaluation of the first sexing strains of C. hominivorax that produce only males when raised on diet without tetracycline. Transgenic lines have been developed that possess a tetracycline repressible female-lethal genetic system. Ten of these lines show high female lethality at the late larval/pupal stages and three of them present dominant female lethality. Most of the lines were comparable to the wild type parental strain in several fitness parameters that are relevant to mass rearing in a production facility. Further, three lines performed well in male mating success and male competition assays, suggesting they would be sexually competitive in the field. Consequently, one transgenic line has been selected by the New World Screwworm Program for evaluation under mass rearing conditions.ConclusionsWe conclude that the promising characteristics of the selected sexing strains may contribute to reduce production costs for the existing eradication program and provide more efficient population suppression, which should make a genetic control program more economical in regions were C. hominivorax remains endemic.Electronic supplementary materialThe online version of this article (doi:10.1186/s12915-016-0296-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

A transgenic male-only strain of the New World screwworm for an improved control program using the sterile insect technique

et al. 2016

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“…The Australian sheep blowfly ( Lucilia cuprina) is an important ecto-parasite that causes fly strike, which has significant health and welfare, as well as economic, impacts on the sheep industry in Australia (Sandeman et al., 2014). The female blowfly is attracted to the sheep by odours, particularly those associated with bacterial infections in damp fleece, and lays eggs (Tellam and Bowles, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…The consequent loss of livestock, costs of preventative and curative chemical treatments, and animal welfare issues place significant economic burdens on livestock enterprises (Lane et al., 2015). The blowfly has developed resistance to various classes of chemical insecticides used for its control, including organochlorines, organophosphates, the benzoyl-phenyl urea diflubenzuron (Levot, 1995, Sandeman et al., 2014) as well as the triazine cyromazine (Levot, 2012). Only two preventative blowfly control chemicals, the macrocyclic lactone ivermectin and the cyanopyrimidine dicyclanil, remain effective with no resistance yet reported.…”

Section: Introductionmentioning

confidence: 99%

Insecticidal activities of histone deacetylase inhibitors against a dipteran parasite of sheep, Lucilia cuprina

Bagnall

Hines

Lucke

et al. 2017

International Journal for Parasitology: Drugs and Drug Resistan

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Histone deacetylase inhibitors (HDACi) are being investigated for the control of various human parasites. Here we investigate their potential as insecticides for the control of a major ecto-parasite of sheep, the Australian sheep blowfly, Lucilia cuprina. We assessed the ability of HDACi from various chemical classes to inhibit the development of blowfly larvae in vitro, and to inhibit HDAC activity in nuclear protein extracts prepared from blowfly eggs. The HDACi prodrug romidepsin, a cyclic depsipeptide that forms a thiolate, was the most potent inhibitor of larval growth, with equivalent or greater potency than three commercial blowfly insecticides. Other HDACi with potent activity were hydroxamic acids (trichostatin, CUDC-907, AR-42), a thioester (KD5170), a disulphide (Psammaplin A), and a cyclic tetrapeptide bearing a ketone (apicidin). On the other hand, no insecticidal activity was observed for certain other hydroxamic acids, fatty acids, and the sesquiterpene lactone parthenolide. The structural diversity of the 31 hydroxamic acids examined here revealed some structural requirements for insecticidal activity; for example, among compounds with flexible linear zinc-binding extensions, greater potency was observed in the presence of branched capping groups that likely make multiple interactions with the blowfly HDAC enzymes. The insecticidal activity correlated with inhibition of HDAC activity in blowfly nuclear protein extracts, indicating that the toxicity was most likely due to inhibition of HDAC enzymes in the blowfly larvae. The inhibitor potencies against blowfly larvae are different from inhibition of human HDACs, suggesting some selectivity for human over blowfly HDACs, and a potential for developing compounds with the inverse selectivity. In summary, these novel findings support blowfly HDAC enzymes as new targets for blowfly control, and point to development of HDAC inhibitors as a promising new class of insecticides.

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“…The species Lucilia cuprina (Wiedemann, 1830) has a cosmopolitan distribution, is the predominant cause of myiasis in sheep and is considered a pest of significant economic importance for neotropical agriculture (Stevens and Wall, 1997;Sandeman et al, 2014). Control of this pest using chemical insecticides is considered largely effective in animal production settings (Wall, 2012;Sandeman et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Control of this pest using chemical insecticides is considered largely effective in animal production settings (Wall, 2012;Sandeman et al, 2014). However, there is a need for complementary control measures in urban settings, where the synanthropic nature of this fly makes it a potential disseminator of human pathogens (Paes et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of larvicidal activity and effects on post embrionary development of laboratory reared Lucilia cuprina (Wiedemann, 1830) (Diptera: Calliphoridae), treated with Brevibacillus laterosporus

Pessanha

Carramaschi

Mallet

et al. 2015

Journal of Invertebrate Pathology

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Control of the sheep blowfly in Australia and New Zealand – are we there yet?

Cited by 50 publications

References 123 publications

A transgenic male-only strain of the New World screwworm for an improved control program using the sterile insect technique

A transgenic male-only strain of the New World screwworm for an improved control program using the sterile insect technique

Insecticidal activities of histone deacetylase inhibitors against a dipteran parasite of sheep, Lucilia cuprina

Evaluation of larvicidal activity and effects on post embrionary development of laboratory reared Lucilia cuprina (Wiedemann, 1830) (Diptera: Calliphoridae), treated with Brevibacillus laterosporus

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