Control of tryptophan synthetase amplified by varying the numbers of composite plasmids in Escherichia coli cells

Nagahari, Kenji; Takano, Teruo; Hishinuma, Fumio; Kuroda, Motoko; Sakaguchi, Kenji

doi:10.1016/0378-1119(77)90025-7

Cited by 58 publications

(27 citation statements)

References 15 publications

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“…Fill-in experiments were performed by using four dNTPs and DNA polymerase I (large fragment) as described (Yoshikawa et al, 1981). DNA sequence analysis 5' or 3' ends of DNA were labeled as described above and subjected to sequence analysis according to Maxam and Gilbert (1980 (Nagahari et al, 1977) to ampicillin resistance (50 /kg/ml).…”

Section: Cloning Of Terminal Dna Fragmentsmentioning

confidence: 99%

A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs.

Hirochika¹,

Nakamura²,

Sakaguchi³

1984

The EMBO Journal

109

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The terminal structure of a linear plasmid pSLA2, which was isolated from Streptomyces rochei, was analysed. The 5' ends of pSLA2 DNA were blocked by the association of a protein probably covalently bonded with the DNA. This block is removed by alkali treatment and blunt ends with 5' -phosphate and 3' -hydroxy termini were released. The two terminal fragments of pSLA2 were cloned and the nucleotide sequence was determined. An inverted terminal repetition of 614 bp was found along with the presence of further interrupted homologous sequences beyond this area up to 800 bp. These are the first inverted terminal repeat sequences found in microbial linear plasmids.

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Section: Cloning Of Terminal Dna Fragmentsmentioning

confidence: 99%

A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs.

Hirochika¹,

Nakamura²,

Sakaguchi³

1984

The EMBO Journal

109

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“…Escherichia coli C600 (rk-mk-thi thr leuB typB) used for transformation was described by Nagahari et al (22). E. coli JM103 (Alacpro thi strA supE endA sbcB15…”

Section: Introductionmentioning

confidence: 99%

“…Escherichia coli C600 (rk-mk-thi thr leuB typB) used for transformation was described by Nagahari et al (22) (24), except that Pronase and phenol treatments were carried out in half the volume of the original bacterial culture. E. coli plasmid DNA was prepared by the alkaline method (25).…”

mentioning

confidence: 99%

Nucleotide sequence of the insertion sequence found in the T-DNA region of mutant Ti plasmid pTiA66 and distribution of its homologues in octopine Ti plasmid.

Machida¹,

Sakurai²,

Kiyokawa³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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The octopine tumor-inducing (Ti) plasmid pTiA66 has an insertion mutation in its T region (the DNA region incorporated into the plant genome) that results in the slow growth of crown gall tumors. These tumors exhibit hormonal autonomy different from that of the crown gall tumors caused by wild-type Ti plasmids. In the present study, the nucleotide sequences of both the DNA segment inserted into pTiA66 and its target site have been determined. The inserted segment is 2548 base pairs long and has 20-base-pair terminal inverted repeats. An 8-base-pair sequence at the target site is duplicated at both integration junctions. These structural features of the insert suggest that it is a bacterial insertion sequence (IS) element, which we have named IS66. Blot-hybridization analyses using IS66 probes revealed that genomes of octopine Ti plasmids contain at least three sequences homologous to IS66: two homologues are located in the virulence region and one is located between the left-hand (TL-DNA) and right-hand (TR-DNA) portions of T-DNA. The chromosome of Agrobacterium tumefaciens A66 also contains two sequences highly homologous to IS66. These results suggest that the mutant pTiA66 plasmid was generated by translocation of one of the sequences showing homology with IS66 into the T region. The fact that a sequence homologous to IS66 is present between TL-DNA and TR-DNA also suggests that the octopine T region was split into two portions, TL-DNA and TR-DNA, by translocation of IS66 or its relatives. Thus, IS66 may cause genetic and structural variations of the T region and the vir region of the octopine Ti plasmids.Tumor-inducing (Ti) plasmids harbored by oncogenic strains of Agrobacterium tumefaciens cause formation of tumors on dicotyledonous plants, called crown galls (1). Cells isolated from the crown gall tumors are characterized by their unlimited proliferation in medium lacking phytohormones, such as cytokinins and auxins, that are required for the culture of normal plant cells (2). It has been shown that a specific DNA region of the Ti plasmid, called the T-DNA region (Fig. 1), causes tumor formation when transferred into plant chromosomes and that the T-DNA persists in the chromosomes of the tumor cells (3-6). The virulence (vir) region of the Ti plasmid (Fig. 1) is located outside the T region and is also required for tumorigenesis by the Ti plasmid. However, this region is probably not stably maintained in the tumor cells (7-10).The Ti plasmids are classified on the basis of the novel amino acid metabolites, such as octopine or nopaline, whose syntheses are directed by T-DNA genes in the tumor cells (11-15). The octopine T region is composed of twQ DNA segments near each other in the Ti plasmid (16) (Fig. 1), whereas the nopaline T region consists of one contiguous DNA segment (17, 18). The chromosomes of the tumor cells caused by the octopine strain contain either one or two portions of the T region (16). All tumor cells that have been analyzed contained the left portion of T-DNA (TL-DNA) but not all...

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“…RSF2124-trp plasmid 7 ) containing the whole E. coli tryptophan operon was used as a source of DNA for the trp promoter and a trpE (anthranilate synthetase) gene. p YT3-24 plasmid, a derivative of a plasmid containing human ACTH-fj-LPH precursor gene,4) was constructed by Teranishi (unpub-lished) and was used for the fJ-EP gene.…”

Section: Methodsmentioning

confidence: 99%

“…The structural gene for E. coli trpE which codes for anthranilate synthetase is 1560 base pairs (bp) long and has two BglII sites at positions 965 and 1026 (A of the ATG for fMet of TrpE being 1).11) RSF2124-trp plasmid 7 ) consisting of E. coli plasmid RSF2124, the whole E. coli tryptophan operon, and AP L and N genes of A phage yields a 2.3 Kb fragment upon digestion with BglII. This fragment was proved to have the AP L promoter, N gene of A phage, the E. coli trp promoter (P T ), and part of the trpE gene (Fig.…”

Section: Construction Of the Expression Vectormentioning

confidence: 99%

High-level Production of Human β-Endorphin in Escherichia coli

Nagahari

Hishida

Nki

et al. 1987

Agricultural and Biological Chemistry

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Control of tryptophan synthetase amplified by varying the numbers of composite plasmids in Escherichia coli cells

Cited by 58 publications

References 15 publications

A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs.

A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs.

Nucleotide sequence of the insertion sequence found in the T-DNA region of mutant Ti plasmid pTiA66 and distribution of its homologues in octopine Ti plasmid.

High-level Production of Human β-Endorphin in Escherichia coli

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