Control of Verticillium wilt of olive by resistant rootstocks

Bubici, Giovanni; Cirulli, M.

doi:10.1007/s11104-011-1002-9

Cited by 58 publications

(50 citation statements)

References 39 publications

(39 reference statements)

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“…Resistant rootstocks would be of much interest for production of Verticillium ‐susceptible olive cultivars in soils infested with D V. dahliae , and efforts have been made in the last few decades to identify olive clones of use for that purpose (Wilhelm, ). Recently, grafting susceptible cultivars Coratina and Leccino onto resistant rootstock Frantoio provided excellent disease control to root dip inoculation with D V. dahliae under artificial conditions (Bubici & Cirulli, ). However, Frantoio and other resistant cultivars were disregarded for use as resistant rootstocks in heavily infested soils because local clones of those cultivars developed a noticeable amount of disease in a soil with moderate inoculum density of D V. dahliae (Trapero et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of resistance against the olive‐defoliating Verticillium dahliae pathotype in selected clones of wild olive

et al. 2016

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Verticillium wilt of olive is best managed by resistant cultivars, but those currently available show incomplete resistance to the defoliating (D) Verticillium dahliae pathotype. Moreover, these cultivars do not satisfy consumers' demand for high yields and oil quality. Highly resistant rootstocks would be of paramount importance for production of agronomically adapted and commercially desirable olive cultivars in D V. dahliae‐infested soils. In this work, resistance to D V. dahliae in wild olive clones Ac‐13, Ac‐18, OutVert and StopVert was assessed by quantifying the fungal DNA along the stem using a highly sensitive real‐time quantitative polymerase chain reaction (qPCR) protocol and a stem colonization index (SCI) based on isolation of V. dahliae following artificial inoculations under conditions highly conducive for verticillium wilt. Ac‐13, Ac‐18, OutVert and StopVert showed a symptomless reaction to D V. dahliae. The mean amount of D V. dahliaeDNA quantified in stems of the four clones ranged from 3.64 to 28.89 pg/100 ng olive DNA, which was 249 to 1537 times lower than that in susceptible Picual olive. The reduction in the quantitative stem colonization of wild olive clones by D V. dahliae was also indicated by a sharp decrease in the SCI. Overall, there was a pattern of decreasing SCI in acropetal progression along the plant axis, as well as correlation between positive reisolation and quantification of pathogen DNA. The results of this research show that wild olive clones Ac‐13, Ac‐18, OutVert and StopVert have a valuable potential as rootstocks for the management of verticillium wilt in olive.

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Section: Introductionmentioning

confidence: 99%

Characterization of resistance against the olive‐defoliating Verticillium dahliae pathotype in selected clones of wild olive

et al. 2016

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“…Screening for Verticillium wilt resistance has also been carried out in wild olive materials, which allowed the selection of new sources of resistance to the disease (Mercado-Blanco et al 2003;Colella et al 2008;Jiménez-Díaz et al 2012). The use of resistant materials as rootstocks for grafting of VW-susceptible olive cultivars has been successfully reported, providing significant reduction on the severity of the attacks (Porras-Soriano et al 2003;Bubici and Cirulli 2012). However, no source of total resistance has been found and all plant materials tested up to now have been infected by the pathogen, so that complete control of VW of olive by means of resistant rootstock cannot be guaranteed, at least in soils heavily infested with V. dahliae.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Verticillium wilt resistance in selections from olive breeding crosses

et al. 2015

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Verticillium wilt (VW) resistance was evaluated in genotypes from olive crosses including resistant cultivars as parents. Thirty-eight genotypes from three crosses were evaluated: 'Changlot Real' 9 'Dolce Agogia' (16), 'Frantoio' 9 'Arbosana' (13) and 'Koroneiki' 9 'Empeltre' (9). These genotypes were previously selected for several agronomic traits from wider initial progenies populations. Several disease severity and plant colonization parameters were evaluated in inoculation experiments under controlled conditions by dipping roots cutting in a conidial suspension of a highly virulent defoliating isolate of Verticillium dahliae. Significant differences among the evaluated genotypes, including parents and selections from crosses, were obtained for all the disease parameters assessed. A wide variability in disease parameters was observed in the three cross combinations tested. Genotypes with lower relative susceptible index values than both parents were found in the three progenies tested and 10 out of 38 genotypes (26 %) were finally classified as resistant. The level of resistance of these genotypes will be confirmed in future studies under field conditions.

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“…). Similarly, biochemical defence reactions took place mainly in the roots during grafting a Verticillium ‐susceptible olive cultivar on rootstock of resistant cultivar (Bubici and Cirulli ). Also, the activation of defence mechanisms in the roots of SA‐pretreated date palm plants was observed as a response to inoculation with the wilt fungus Fusarium oxysporum f.sp.…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of Potato Defence Genes Associated with the Salicylic Acid Defence Signalling Pathway in Response to Weakly and Highly Aggressive Isolates of Verticillium dahliae

Derksen

Badawi

Henríquez

et al. 2012

Journal of Phytopathology

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Verticillium wilt is a vascular disease affecting hundreds of important dicotyledonous crops worldwide. Its main causal agent in potato is Verticillium dahliae Kleb. A differential potato‐V. dahliae system consisting of two cultivars of potato (susceptible; S and moderately resistant; MR) and two V. dahliae isolates (weakly, WA and highly aggressive, HA), was used to evaluate the expression of five defence‐related genes, PAL1, PAL2, PR‐1, PR‐2 and PR‐5. These genes were selected because they are in general associated with the salicylic acid defence signalling pathway. Expression levels of these genes were assessed in potato roots and leaves at 0, 4 and 21 h (hpi), and 3, 7 and 14 days postinoculation (dpi). In the roots, the expression of PAL1, PR‐1 and PR‐2 in the MR was higher than in the susceptible cultivar in response to inoculation with either one of the tested V. dahliae isolates. PAL2 gene expression increased gradually starting at 21 hpi in the MR cultivar as opposed to the susceptible one. Higher expression of PR‐1 was detected at 7 dpi in roots. In the leaves, both PAL1 and PAL2 genes showed higher expression in the MR cultivar relative to the susceptible one. Interestingly, the expression of PR‐2 was slightly higher in the susceptible cultivar. Combined data analyses revealed that PAL1, PAL2, PR‐1 and PR‐2 genes are regulated at the transcriptional level in response to infection by V. dahliae. These results indicate that the salicylic acid pathway is also involved in potato defence against V. dahliae and add to the data gathered to elucidate the signalling mechanisms in this host–pathogen interaction.

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Control of Verticillium wilt of olive by resistant rootstocks

Abstract: Aims The potential of grafting susceptible cultivars onto resistant rootstocks was evaluated for the control of Verticillium wilt in olive.

Cited by 58 publications

References 39 publications

Characterization of resistance against the olive‐defoliating Verticillium dahliae pathotype in selected clones of wild olive

Characterization of resistance against the olive‐defoliating Verticillium dahliae pathotype in selected clones of wild olive

Evaluation of Verticillium wilt resistance in selections from olive breeding crosses

Differential Expression of Potato Defence Genes Associated with the Salicylic Acid Defence Signalling Pathway in Response to Weakly and Highly Aggressive Isolates of Verticillium dahliae

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