Control of Viruses Infecting Grapevine

Maliogka, Varvara I.; Martelli, G. P.; Fuchs, Marc; Katis, N. I.

doi:10.1016/bs.aivir.2014.11.002

Cited by 110 publications

(99 citation statements)

References 130 publications

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“…So far, several techniques have been applied to identify infected plant material, including biological indexing, serology and molecular assays [3,83,99].…”

Section: Diagnostic Methodsmentioning

confidence: 99%

“…Serological methods are based on the recognition of viral proteins by specific antibodies. Of these, the enzyme-linked immunosorbent assay (ELISA) is the most widely applied [83,99], and commercial kits are available. Serological approaches are robust and scalable, although less sensitive than nucleic acid-based techniques.…”

Section: Diagnostic Methodsmentioning

confidence: 99%

“…Several techniques have been developed based on PCR variants [83,102], but the use of real-time PCR allows quantification of virus titer [103]. Recently, new generation sequencing (NGS) has been used for rapid identification and sequencing of all putative viruses present in a candidate sample, allowing the identification of new viral agents as well [3,81,99,104]. The use of NGS technologies as diagnostic tool requires no prior knowledge of the pathogens present in the sample, but is still expensive in order to be used as a routine procedure.…”

Section: Diagnostic Methodsmentioning

confidence: 99%

“…For instance, sanitary selection and certification of propagation material helps to reduce potential virus dispersion [99]. Since viruses are transmitted by vectors, control of viral diseases can be achieved by the restriction of such vectors with the use of agrochemicals [105].…”

Section: Control Methodsmentioning

confidence: 99%

“…Several papers describe viral detection by molecular approaches, which are reviewed in [83,99]. However, a simple and efficient commercial method for the detection of several grapevine viruses at once is currently not available.…”

Section: Multiplex Pcr To Detect Complex Viral Infectionsmentioning

confidence: 99%

See 4 more Smart Citations

Grapevine Biotechnology: Molecular Approaches Underlying Abiotic and Biotic Stress Responses

Armijo¹,

Espinoza²,

Loyola³

et al. 2016

Grape and Wine Biotechnology

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Grapevine is one of the most abundant crops worldwide, with varieties destined for fresh and dry consumption, as well as wine production. Unfortunately, grapevine plants are affected by both biotic and abiotic stresses, generating significant economic losses. These conditions can negatively impact grape cultivation at different stages: plant and berry development during pre-and post-harvest, production, fresh fruit processing and export, along with wine quality. Most of the grapevine varieties are susceptible to several pathogens and within this chapter, particular attention is given to fungi (Botrytis cinerea and Erysiphe necator) and viruses, since they are a worldwide concern. Within the latter, special focus is given to the grapevine leafroll disease, a complex and destructive infection. On the other hand, abiotic stress is also relevant in grapevine, and in this chapter it will be exemplified by UV-B radiation and its impact on growth and fruit development, plant adaptive responses and its relationship with the quality of grape berries for winemaking. The main biotic and abiotic grapevine stress factors are reviewed in this chapter, considering a special focus on biotechnological approaches carried out in order to address them and minimize their detrimental consequences.

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“…So far, several techniques have been applied to identify infected plant material, including biological indexing, serology and molecular assays [3,83,99].…”

Section: Diagnostic Methodsmentioning

confidence: 99%

Section: Diagnostic Methodsmentioning

confidence: 99%

Section: Diagnostic Methodsmentioning

confidence: 99%

Section: Control Methodsmentioning

confidence: 99%

Section: Multiplex Pcr To Detect Complex Viral Infectionsmentioning

confidence: 99%

See 3 more Smart Citations

Grapevine Biotechnology: Molecular Approaches Underlying Abiotic and Biotic Stress Responses

Armijo¹,

Espinoza²,

Loyola³

et al. 2016

Grape and Wine Biotechnology

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Virus Diseases of Grapevine

Martelli¹

2019

Encyclopedia of Life Sciences

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There are 25 recognised virus and virus‐like diseases of grapevines ( Vitis and Muscadinia spp.). Many have a clear‐cut detrimental effect on the vines, that is stunting, reduced vigour, malformation of the leaves and twigs, foliar discolourations ranging from reddening to chlorotic or bright yellow mottling, ringspots and line patterns. The productive life of the vineyards can be shortened and the quantity and quality of the crop badly affected. Prevailing disease agents are viruses transmitted by nematodes (nepoviruses and Strawberry latent ringspot virus) , pseudococcid mealybugs and soft‐scale insects (closteroviruses and vitiviruses). Infected propagation materials (scions and rootstocks) are the major way for long‐distance dissemination of diseases, several of which have now a worldwide distribution and have entered areas where the grapevine industry is expanding. Control is based on preventive measures such as sanitary selection, sanitation and induction of resistance through conventional breeding or, to come in the future, genetic engineering. Key Concepts Virus and virus‐like diseases are a major threat to the world's grapevine industry. The world of grapevine‐infecting viruses is the most variegated among woody crops. Prevailing viruses are soil‐borne and mealybug‐transmitted. Spreading of diseases and their agents is mediated by infected propagative material and vectors. Nursery productions have played, and still do, a major role in disease dissemination. Disease control relies on preventive measures aimed at reducing vector populations and sources of inoculum. Sanitary selection and sanitation are most efficient means for producing certified stocks.

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Foliar Delivery of siRNA Particles for Treating Viral Infections in Agricultural Grapevines

Avital

Muzika

Persky

et al. 2021

Adv Funct Materials

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Grapevine leafroll disease (GLD) is a globally spreading viral infection thatcauses major economic losses by reducing crop yield, plant longevity, and berry quality, with no effective treatment. Grapevine leafroll associated virus-3 (GLRaV-3) is the most severe, prevalent GLD strain affecting wine production. Here, the ability of RNA interference (RNAi), a non-GMO gene-silencing pathway, to treat GLRaV-3 in infected Cabernet Sauvignon grapevines is evaluated. Lipid-modified polyethylenimine (lmPEI) is synthesized as the carrier for long double-stranded RNA (dsRNA, 250-bp-long) that targets RNA polymerase and coat protein is a gene target that are conserved in the GLRaV-3 genome. Self-assembled dsRNA-lmPEI particles, 220 nm in diameter, display inner ordered domains spaced 7.3 ± 2 nm from one another, correlating to lmPEI wrapping spirally around the dsRNA. The particles effectively protect RNA from degradation by ribonucleases and show to increase uptake rate into plant cells as a result of the lipid component comprising the RNA carrier. In three field experiments, a single dose of foliar sprayed treatment of the RNA-particles knocks down GLRaV-3 titer, and multiple doses of the treatment keep the viral titer at baseline and trigger recovery of the vine and berries. This study demonstrates RNAi as a promising platform for treating viral diseases in agriculture.

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Control of Viruses Infecting Grapevine

Cited by 110 publications

References 130 publications

Grapevine Biotechnology: Molecular Approaches Underlying Abiotic and Biotic Stress Responses

Grapevine Biotechnology: Molecular Approaches Underlying Abiotic and Biotic Stress Responses

Virus Diseases of Grapevine

Foliar Delivery of siRNA Particles for Treating Viral Infections in Agricultural Grapevines

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