In this study, the vehicle seat suspension system installed with a semi-active magnetorheological (MR) damper is excited by the power spectral density (PSD) and its vibration control performance is evaluated using finite element analysis (FEA). The control system receives the velocity of the sprung and unsprung mass from the seat mounting locations and calculates the desired controllable damping forces available from MR damper. The effectiveness of the control systems is demonstrated by adopting ride quality evaluation method. To predict a better ride comfort and ride quality, co-simulation methodology is utilized considering the dynamic behavior of the real-world seat system. In this case, the multi-body dynamics and control system are coupled by solving the mechanical equations and the control logic. Then, the computed damping force is exchanged between the dynamics and controller in an iterative manner by passing state variables. The simulation results achieved from co-simulation methodology associated with the controller are analyzed by comparing with ISO 2631-1. The co-simulation results studied using vibration dose value (VDV), crest factor (CF) and seat effective amplitude transmissibility (SEAT) in terms of percentage improvement were found to be 44.33%, 26.66% and 17.65% respectively which were better than that for the passive suspension for a random rough road profile. Modified skyhook controller delivers superior performance for vibration suppression of the vehicle seat suspension as compared to other control policies. The application of co-simulation methodology can reduce time and cost for the development of a semi-active seat suspension system.