Refrigeration and air conditioning systems consume high rates of electrical energy of the total global power consumption. The major part of this energy is used by compressor which is the main equipment in vapor compression refrigeration systems. In this study, the performance of a variable speed chilled water refrigeration system with electronic expansion valve (EEV) is experimentally investigated. The system is composed of variable speed rotary compressor, water cooled condenser, electronic expansion valve, and evaporator with refrigerant R410a for one tone cooling capacity. The results showed that the EEV opening was related to the compressor speed at limits of refrigerant subcool and system stability to achieve better performance. Refrigerant superheats increased with closing the EEV at constant compressor speed. Moreover, the degree of superheat was inversely proportional to the compressor speed at constant EEV opening. The coefficient of performance (COP) was improved by about 2.2 to 4.0% by controlling the EEV at constant compressor speed. Increasing compressor speed from 1200 to 3600 rpm resulted in decreasing system COP from 5.2 to 2.35 due to the increase of the power consumed by compressor.