Controle de Alternaria solani em tomateiro por extratos de Curcuma longa e curcumina: I. avaliação in vitro

Balbi-Peña, María Isabel; Becker, Andrea; Stangarlin, José Renato; Franzener, Gilmar; Lopes, Mário César; Schwan-Estrada, Kátia Regina Freitas

doi:10.1590/s0100-41582006000300012

Cited by 30 publications

(19 citation statements)

References 9 publications

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“…The number of conidia was determined with a Neubauer chamber in a light microscope (2). All tests were conducted with 4 replicates.…”

Section: Evaluation Of Conidia Production On Neubauer Chambermentioning

confidence: 99%

“…Since conidia production by some A. alternata isolates in culture media requires special techniques (3,30), simple methods to do so are greatly welcome (2,16). Thus, as part of a project aimed to carry out several studies with such fungus, this work was directed towards the establishment of a new methodology to easily obtain A. alternata conidia in a culture medium.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of methodologies for conidia production by Alternaria alternata from citrus

Carvalho

Alves

Batista

et al. 2008

Braz. J. Microbiol.

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Conidia production is a problem in the study of Alternaria alternata from citrus. Thus, this study aimed to compare existing methodologies for conidial production of A. alternata isolated from Ponkan tangerine (2 isolates), Cravo lemon (1 isolate), Pêra orange (2 isolates) and Murcott tangor (1 isolate). The methodologies used were conidia production with 12 and 24 hours under white fluorescent light, evaluation with 24 and 48 hours after applying fungal mycelium stress technique, cold stress followed by injury of mycelium and evaluation with 24 hours, using healthy vegetable tissue and the use of black fluorescent near ultraviolet (NUV) lamp. Satisfactory result was obtained with A. alternata isolate from Murcott tangor, with the production of 2.8 x 10 5 conidia mL -1, when fungal mycelium was stressed (Petri dish with 66.66% of fungi growth) and subsequently 24 h of growth. The use of white light (24 h) and black fluorescent NUV lamp also induced expressive conidia production by one isolate of Ponkan tangerine, which produced 17.2 x 10 5 and 10.1 x 10 5 conidia mL -1 and another of Murcott tangor, which produced 13.9 x 10 5 and 10.1 x 10 5 conidia mL -1, respectively. The remaining methodologies analyzed in this study were not able to induce conidia production in satisfactory quantity. The use of both mycelium stress technique and white light (24 h) and black fluorescent NUV lamp allowed the production of enough quantities of conidia to be used in vitro (detection of fungitoxic substances) and in vivo (pathogenicity test) assays, respectively.

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“…The number of conidia was determined with a Neubauer chamber in a light microscope (2). All tests were conducted with 4 replicates.…”

Section: Evaluation Of Conidia Production On Neubauer Chambermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of methodologies for conidia production by Alternaria alternata from citrus

Carvalho

Alves

Batista

et al. 2008

Braz. J. Microbiol.

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“…A atividade antifúngica de A. camphorata também foi verificada por Franzener et al (2003), sendo que o extrato aquoso de 50% causou a inibição de 39% do crescimento micelial de B. sorokiniana, e a concentração de 10% já inibiu completamente a esporulação. Especificamente para A. solani, o trabalho de Balbi-Peña et al (2006a) indicou que os extratos não autoclavados de Curcuma longa a 10% e 15% inibiram o crescimento micelial em 38,2 e 23,2%, respectivamente, e a esporulação em 71,7 e 87%, respectivamente.…”

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Atividade antifúngica e proteção do tomateiro por extratos de plantas medicinais

Itako

Schwan-Estrada

Júnior

et al. 2008

Trop. plant pathol.

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RESUMOEste trabalho teve como objetivos avaliar, in vitro, a fungitoxicidade dos extratos brutos aquosos (EBAs) de Achillea millefolium, Artemisia camphorata, Cymbopogon citratus e Rosmarinus officinalis contra Alternaria solani e o efeito protetor destes extratos em plantas de tomateiro em casa-de-vegetação. Para verificar a atividade antifúngica, os EBAs foram incorporados ao BDA e avaliadas a inibição do crescimento micelial, a esporulação e a germinação de conídios. O efeito protetor em plantas foi avaliado através da pulverização preventiva (72h antes da inoculação) dos EBAs, nas concentrações de 10 e 20%, nas duas primeiras folhas. A severidade da doença foi verificada 15 dias após a inoculação. Verificou-se que os EBAs não inibiram o crescimento micelial, mas tiveram efeitos significativos na redução da esporulação e da germinação de conídios, principalmente os EBAs de A. camphorata, C. citratus e R. officinalis, a partir da concentração de 20%. Já em relação à proteção das plantas verificou-se uma redução no número de lesões em relação à testemunha, nas folhas acima das tratadas, observando o efeito sistêmico dos extratos. Os extratos estudados podem ser promissores no controle da pinta-preta em tomateiro. Palavras-chave: Alternaria solani, controle alternativo. ABSTRACT Antifungal activity and protection of tomato plants by extracts of medicinal plantsThe present work aimed to evaluate, in vitro, the fungitoxicity of aqueous crude extracts (ACEs) of Achillea millefolium, Artemisia camphorata, Cymbopogon citratus and Rosmarinus officinalis to Alternaria solani and their protective effect on tomato plants under greenhouse conditions. To evaluate the antifungal activity of ACEs, they were incorporated into potato-dextrose-agar medium and the inhibition of mycelial growth, sporulation and conidia germination was evaluated. The protective effect in plants was evaluated by means of preventive spraying (72 hours before inoculation) with ACE, at concentrations of 10 and 20%, in the first two leaves. Disease severity was verified 15 days after inoculation. It was observed that ACEs did not inhibit mycelial growth, but they had significant effects on the reduction of germination and sporulation, especially the ACEs of A. camphorata, C. citratus and R. officinalis, at concentrations up to 20%. A reduction was observed in the number of lesions in relation to the control, in leaves above those treated, observing the systemic effect of the extracts. The extracts from the studied plants may be promising in the control of early blight on tomato plants.

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“…This was recovered by subculture to Petri dishes containing about 20 mL PDA (potato dextrose agar), followed by new subculture to V8-agar medium. The isolate was incubated at 25°C and 12 h Toledo et al 3825 photoperiod (Balbi-Peña et al, 2006).…”

Section: Obtaining a Solani Isolatesmentioning

confidence: 99%

Fungitoxicity activity of homeopathic medicines on Alternaria solani

Toledo¹,

Stangarlin²,

Bonato³

et al. 2016

Afr. J. Agric. Res.

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The black spot disease caused by the fungus Alternaria solani (Ellis and Martin) L. R. Jones and Gront is one of the most important diseases of tomato (Lycopersicon esculentum Mill.). The control is usually performed with fungicides, resulting in products contaminated with pesticide residues. In recent years, the use of homeopathic medicines has been highlighted in research for disease control. The aim of this study was to evaluate the in vitro fungitoxicity against A. solani by the homeopathic medicines Propolis, Isotherapic of A. solani and Isotherapic of ash, at 6, 12, 30 and 60CH (hahnemanian centesimal) dynamizations, and Sulphur, Silicea terra, Staphysagria, Phosphorus, Ferrum sulphuricum and Kali iodatum at 6, 12, 30 and 100CH dynamizations. Distilled water and 30% hydroalcoholic solution were used as controls at 12, 30, 60 and 100CH dynamizations. Mycelial growth, sporulation and conidial germination of A. solani were evaluated. The results indicated that for mycelial growth only in Sulphur and Staphysagria 100CH showed suppressive effect compared to both controls. For sporulation, Propolis 6, 30 and 60CH and Ferrum sulphuricum 6 and 30CH caused inhibition and differed from both controls. Isotherapic of A. solani 6CH, Isotherapic of ash 6CH and Ferrum sulphuricum 30CH reduced spores germination of the pathogen. It was also found that distilled water at 60 and 100CH inhibited mycelium growth. These results indicate the potential of some homeopathic medicines for trials aiming to control the black spot disease in tomato crops.

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Controle de Alternaria solani em tomateiro por extratos de Curcuma longa e curcumina: I. avaliação in vitro

Cited by 30 publications

References 9 publications

Comparison of methodologies for conidia production by Alternaria alternata from citrus

Comparison of methodologies for conidia production by Alternaria alternata from citrus

Atividade antifúngica e proteção do tomateiro por extratos de plantas medicinais

Fungitoxicity activity of homeopathic medicines on Alternaria solani

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