Controle do desenvolvimento vegetal pela interação auxina-citocinina. Uma nova abordagem baseada no estudo de mutantes de tomateiro (Solanun lycopersicum cv Micro-Tom)

Pino-Nunes, Lilian Ellen

doi:10.11606/t.64.2009.tde-15102009-112355

Cited by 2 publications

(1 citation statement)

References 139 publications

(202 reference statements)

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“…In addition to the application of CKs and the use of substances that may stimulate CK biosynthesis, such as Si, plants that overexpress the enzyme cytokinin oxidase and consequently exhibit reduced levels of CKs are important models used to generate information on the processes regulated by CKs (Werner et al 2001). In this context, the tomato-dwarf cultivar Micro-Tom (MT) was genetically transformed to overexpress the enzyme CYTOKININ OXI-DASE 2 (MT CKX2) (Pino-Nunes 2009). Thus, we hypothesized that (i) Si alleviates NH 4 + toxicity in MT but not in MT CKX2, (ii) the attenuation of NH 4 + toxicity by Si is dependent on increased levels of CKs, and (iii) 6-BA alleviates NH 4 + toxicity in tomato MT.…”

Section: Introductionmentioning

confidence: 99%

Ammonium Toxicity Alleviation by Silicon is Dependent on Cytokinins in Tomato cv. Micro-Tom

Barreto

Prado

Lúcio

et al. 2021

J Plant Growth Regul

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The objectives were to verify the effects of the lack of cytokinins (CKs), comparing tomato cv. Micro-Tom (MT, wild type) to MT CKX2 (transgenic with less CKs) fed with nitrate (NO 3 − ) and ammonium (NH 4 + ), in the presence and absence of silicon (Si); verify if the attenuation of NH 4 + toxicity by Si depends on the increase of CKs in MT; and verify if 6-benzyladenine (6-BA) attenuates NH 4 + toxicity in MT. Three experiments were performed with treatments via nutrient solution. First, MT and MT CKX2 were grown with NO 3 − or NH 4 + (5.9 mmol L − 1 ), in the absence and presence of Si (1.28 mmol L − 1 ). Second, MT was grown with NO 3 − or NH 4 + (5.9 mmol L − 1 ), in the absence and presence of Si (1.28 mmol L − 1 ). Third, MT was grown with NO 3 − or NH 4 + (5.9 mmol L − 1 ) and 6-BA (from 1e −10 to 1e −6 mol L −1 ) associated with NH 4 + . The MT and MT CKX2 had a decrease of 18% and 48% in the shoot dry weight, respectively, when fed with NH 4 + , compared to NO 3 − . Si attenuated NH 4 + toxicity in MT, but not in MT CKX2. This attenuation in MT was accompanied by a decrease in transzeatin (tZ) content in the roots and increase in the shoots. 6-BA did not improve the shoot growth of MT fed with NH 4 + . In conclusion, the alleviation of NH 4 + toxicity by Si was dependent on the increase in tZ content in shoots. In CK-deficient plants, Si did not alleviate NH 4 + toxicity, and 6-BA did not alleviate NH 4 + toxicity in MT shoots.

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Section: Introductionmentioning

confidence: 99%

Ammonium Toxicity Alleviation by Silicon is Dependent on Cytokinins in Tomato cv. Micro-Tom

Barreto

Prado

Lúcio

et al. 2021

J Plant Growth Regul

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Genômica funcional da interação cacaueiro (Theobroma cacao L.) x Moniliophthora perniciosa por meio do sistema modelo Micro-Tom (Solanum lycopersicum L.)

Scotton¹

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pela orientação, pelas valiosas contribuições científicas e acadêmicas, pela oportunidade e amizade. Ao Prof. Dr. Antonio Figueira, pela orientação, disponibilidade do laboratório, amizade, confiança, pelo meu desenvolvimento profissional e paciência durante os oito anos de convivência. A CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), pela concessão de bolsa de estudo de doutorado e a FAPESP (Fundação de Amparo a Pesquisa do Estado de São Paulo) pelo apoio financeiro necessário para a realização desta pesquisa. Ao Centro de Energia Nuclear na Agricultura (CENA/USP), pela oportunidade de realização do doutorado. Aos Professores do CENA, que compõem a área de concentração Biologia na Agricultura e no Ambiente e aos Professores das disciplinas cursadas. Aos colegas da Pós-graduação pelo convívio e aprendizado mútuo.

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Controle do desenvolvimento vegetal pela interação auxina-citocinina. Uma nova abordagem baseada no estudo de mutantes de tomateiro (Solanun lycopersicum cv Micro-Tom)

Cited by 2 publications

References 139 publications

Ammonium Toxicity Alleviation by Silicon is Dependent on Cytokinins in Tomato cv. Micro-Tom

Ammonium Toxicity Alleviation by Silicon is Dependent on Cytokinins in Tomato cv. Micro-Tom

Genômica funcional da interação cacaueiro (Theobroma cacao L.) x Moniliophthora perniciosa por meio do sistema modelo Micro-Tom (Solanum lycopersicum L.)

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