Controllable preparation and high properties of fluorescence and surface enhanced raman spectra encoded poly(glycidyl methacrylate) microsphere

Abstract

Novel fluorescence and SERS encoded microspheres based on poly(glycidyl methacrylate) (PGMA) microspheres were developed by a controllable approach. The microspheres were encoded by four fluorescence dyes and three surface enhanced Raman spectra (SERS) probes. Multiple dyes could be simultaneously incorporated into the PGMA microspheres for encoding and the intensity of the fluorescence can be tuned by adjusting the feeding ratio. The PGMA microspheres were coated by silver nanoparticles and encoded by single and multiple SERS reporters. The fluorescence and SERS joint encoded PGMA microspheres were fabricated. All the fluorescence encoding signals can be excited at 488 nm. The fluorescent and SERS signals of dual-mode microspheres could be obtained from two optical channels and non-overlapping of emission spectra was observed in these encoded microspheres, which greatly increased the encoding capacity. The as prepared fluorescence and SERS encoded microspheres possess stable and distinct spectral encoding signals, large encoding capacity.

Studies on Preparation, Characterization and Application of Porous Functionalized Glycidyl Methacrylate-Based Microspheres

“…Microspheres based on GMA are also widely applied in bioseparation [28,29], cell isolation [30], nucleic acid and protein purification and immunoassay [31][32][33][34]. Fluorescence and encoded GMA-based microspheres can be used as fluorescent sensor [35] and material that shows a particularly important role in labeling, detection, and high-throughput screening [36,37]. This paper discusses the use of a new polymer material containing different polar groups on the surface as a stationary phase for the chromatographic column.…”

“…Microspheres based on GMA are also widely applied in bioseparation [ 28 , 29 ], cell isolation [ 30 ], nucleic acid and protein purification and immunoassay [ 31 , 32 , 33 , 34 ]. Fluorescence and encoded GMA-based microspheres can be used as fluorescent sensor [ 35 ] and material that shows a particularly important role in labeling, detection, and high-throughput screening [ 36 , 37 ].…”

Sub-micron microparticles with tunable fluorescence emission obtained via co-self-assembly of amidoximed polymeric ligands and lanthanide ions

“…According to the different fluorescent substances in the final beads, the polymeric fluorescent microspheres can be generally divided into inorganic fluorophore-polymeric microspheres and organic fluorophore-polymeric microspheres. Among them, the fluorescent microparticles obtained by using semiconductor quantum dots (QDs) ( Jia et al, 2022b ), organic dyes ( You et al, 2019 ), rare earth metals ( Pan et al, 2019 ), are the major types due to their specific advantage. For instance, QDs have stable resistance to photobleaching and narrow emission bands due to their quantum confinement effects ( He et al, 2019 ).…”